High sensitive and selective C-reactive protein detection by means of lossy mode resonance based optical fiber devices
Fecha
2017Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión aceptada / Onetsi den bertsioa
Impacto
|
10.1016/j.bios.2016.09.020
Resumen
This work presents the development of high sensitive, selective, fast and reusable C-reactive protein (CRP) aptasensors. This novel approach takes advantage of the utilization of high sensitive
refractometers based on Lossy Mode Resonances generated by thin indium tin oxide (ITO) films fabricated onto the planar region of D-shaped optical fibers. CRP selectivity is obtained by means of the
adhe ...
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This work presents the development of high sensitive, selective, fast and reusable C-reactive protein (CRP) aptasensors. This novel approach takes advantage of the utilization of high sensitive
refractometers based on Lossy Mode Resonances generated by thin indium tin oxide (ITO) films fabricated onto the planar region of D-shaped optical fibers. CRP selectivity is obtained by means of the
adhesion of a CRP specific aptamer chain onto the ITO film using the Layer-by-Layer (LbL) nano-assembly fabrication process. The sensing mechanism relies on resonance wavelength shifts originated by
refractive index variations of the aptamer chain in presence of the target molecule. Fabricated devices show high selectivity to CRP when compared with other target molecules, such as urea or creatinine,
while maintaining a low detection limit (0.0625 mg/L) and fast response time (61 s). Additionally, these sensors show a repetitive response for several days and are reusable after a cleaning process in
ultrapure water. [--]
Materias
Biosensors,
C-reactive protein,
Optical fiber,
Lossy mode resonance,
D-shaped
Editor
Elsevier
Publicado en
Biosensors and Bioelectronics 93 (2017) 176-181
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute of Smart Cities - ISC /
Universidad Pública de Navarra. Departamento de Ingeniería Eléctrica y Electrónica /
Nafarroako Unibertsitate Publikoa. Ingeniaritza Elektrikoa eta Elektronikoa Saila
Versión del editor
Entidades Financiadoras
This work was supported by a Pre-Doctoral Research Grant of
the Public University of Navarra, Spanish Economy and Competitiveness Ministry-Feder TEC2013-43679-R and Fundación
CAN2015-70221 Research Grants.