Browsing by Author "Mouzo Calzadilla, Daniel"

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    Caracterización de celulas madre hematopoyéticas humanas a través de datos transcriptómicos de célula única
    (2024) Gil Bescós, Rubén; Pumar Méndez, María Jesús; López Pérez, Ana Rosa; Mouzo Calzadilla, Daniel; Facultad de Ciencias de la Salud; Osasun Zientzien Fakultatea
    Annotation is an essential step in the bioinformatic processing of single-cell sequencing data, where each cell is assigned a biological identity. This process is particularly relevant for the analysis of small and heterogeneous opulations, such as progenitor cells. In this study, the annotation of CD34+ cells is performed in samples from mobilized peripheral blood, obtained by Multiome 10X and CITE-Seq sequencing methods. The characterization of transcriptomic and membrane marker data enables the identification of different progenitor populations present in the samples. Additionally, different annotation methodologies are compared, including automatic annotation with SingleR and “Label Transfer”, as well as a manual annotation. Moreover, subpopulations within the hematopoietic stem cells (HSC) are identified, such as long-term HSC (LT-HSC) and short-term HSC (ST-HSC). Lastly, a Shiny application was used to facilitate the visualization of the annotated samples. Thus, this study provides new tools and insights to facilitate the analysis of hematopoietic stem and progenitor cells.