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Ameztoy del Amo, Kinia

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https://academica-e.unavarra.es/handle/2454/48521

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Ameztoy del Amo

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Kinia

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Instituto de Agrobiotecnología (IdAB)

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0000-0003-2369-3690

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811066

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2016 - 20203
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  • Thumbnail Image
    PublicationOpen Access
    Volatile compounds other than CO2 emitted by different microorganisms promote distinct posttranscriptionally regulated responses in plants
    (Wiley, 2019) García Gómez, Pablo; Almagro Zabalza, Goizeder; Sánchez López, Ángela María; Bahaji, Abdellatif; Ameztoy del Amo, Kinia; Ricarte Bermejo, Adriana; Baslam, Marouane; López Gómez, Pedro; Morán Juez, José Fernando; Garrido Segovia, Julián José; Muñoz Pérez, Francisco José; Baroja Fernández, Edurne; Pozueta Romero, Javier; Zientziak; Institute for Advanced Materials and Mathematics - INAMAT2; Ciencias; Gobierno de Navarra / Nafarroako Gobernua
    A 'box-in-box' cocultivation system was used to investigate plant responses to microbial volatile compounds (VCs) and to evaluate the contributions of organic and inorganic VCs (VOCs and VICs, respectively) to these responses. Arabidopsis plants were exposed to VCs emitted by adjacent Alternaria alternata and Penicillium aurantiogriseum cultures, with and without charcoal filtration. No VOCs were detected in the headspace of growth chambers containing fungal cultures with charcoal filters. However, these growth chambers exhibited elevated CO2 and bioactive CO and NO headspace concentrations. Independently of charcoal filtration, VCs from both fungal phytopathogens promoted growth and distinct developmental changes. Plants cultured at CO2 levels observed in growth boxes containing fungal cultures were identical to those cultured at ambient CO2. Plants exposed to charcoal-filtered fungal VCs, nonfiltered VCs, or superelevated CO2 levels exhibited transcriptional changes resembling those induced by increased irradiance. Thus, in the 'box-in-box'' system, (a) fungal VICs other than CO2 and/or VOCs not detected by our analytical systems strongly influence the plants' responses to fungal VCs, (b) different microorganisms release VCs with distinct action potentials, (c) transcriptional changes in VC-exposed plants are mainly due to enhanced photosynthesis signaling, and (d) regulation of some plant responses to fungal VCs is primarily posttranscriptional.
  • Thumbnail Image
    PublicationOpen Access
    Regulación postraduccional de la respuesta de las plantas a volátiles emitidos por hongos fitopatógenos
    (2020) Ameztoy del Amo, Kinia; Pozueta Romero, Javier; Baroja Fernández, Edurne; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa; Gobierno de Navarra / Nafarroako Gobernua
    Con el fin de profundizar en los mecanismos que median la relación entre la planta y los microorganismos, en este trabajo investigué el papel que juegan dos formas de regulación postraduccional en la respuesta de las plantas a los VCs emitidos por el hongo fitopatógeno Alternaria alternata: la regulación redox y la proteostasis. En el trabajo presentado en el capítulo 1 investigué el papel que juega la tiorredoxina reductasa C dependiente de NADPH (NTRC) en la respuesta de las plantas a los VCs microbianos. Para ello, analicé la respuesta en el desarrollo, en el metabolismo y en el redox proteoma de plantas wild type (WT) y un mutante knock out de NTRC (ntrc) a VCs emitidos por A. alternata. Tras hipotetizar que la respuesta de las plantas a VCs microbianos implica una regulación postraduccional de la expresión de las enzimas involucradas en la ruta 2-C-methyl-D-erythritol 4-phosphate (MEP) y del shikimato a través de mecanismos que implican una señalización de la activación redox de la fotosíntesis, en el trabajo presentado en el segundo capítulo comparé las respuestas a VCs fúngicos de plantas de Arabidopsis WT y un mutante cfbp1 que no expresa la isoforma de la enzima fructosa-1,6-bisfosfatasa del ciclo de Calvin-Benson que posee regulación redox.
  • Thumbnail Image
    PublicationOpen Access
    Arabidopsis responds to Alternaria alternata volatiles by triggering pPG-independent mechanisms
    (American Society of Plant Biologists, 2016) Sánchez López, Ángela María; Bahaji, Abdellatif; Diego, Nuria de; Baslam, Marouane; Li, Jun; Muñoz Pérez, Francisco José; Almagro Zabalza, Goizeder; García Gómez, Pablo; Ameztoy del Amo, Kinia; Ricarte Bermejo, Adriana; Novák, Ondrej; Humplik, Jan F.; Spíchal, Lukás; Dolezal, Karel; Ciordia, Sergio; Mena, María Carmen; Navajas, Rosana; Baroja Fernández, Edurne; Pozueta Romero, Javier; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua; Gobierno de Navarra / Nafarroako Gobernua (IIM010491.RI1); Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
    Volatile compounds (VCs) emitted by phylogenetically diverse microorganisms (including plant pathogens and microbes that do not normally interact mutualistically with plants) promote photosynthesis, growth, and the accumulation of high levels of starch in leaves through cytokinin (CK)-regulated processes. In Arabidopsis (Arabidopsis thaliana) plants not exposed to VCs, plastidic phosphoglucose isomerase (pPGI) acts as an important determinant of photosynthesis and growth, likely as a consequence of its involvement in the synthesis of plastidic CKs in roots. Moreover, this enzyme plays an important role in connecting the Calvin- Benson cycle with the starch biosynthetic pathway in leaves. To elucidate the mechanisms involved in the responses of plants to microbial VCs and to investigate the extent of pPGI involvement, we characterized pPGI-null pgi1-2 Arabidopsis plants cultured in the presence or absence of VCs emitted by Alternaria alternata. We found that volatile emissions from this fungal phytopathogen promote growth, photosynthesis, and the accumulation of plastidic CKs in pgi1-2 leaves. Notably, the mesophyll cells of pgi1-2 leaves accumulated exceptionally high levels of starch following VC exposure. Proteomic analyses revealed that VCs promote global changes in the expression of proteins involved in photosynthesis, starch metabolism, and growth that can account for the observed responses in pgi1-2 plants. The overall data show that Arabidopsis plants can respond to VCs emitted by phytopathogenic microorganisms by triggering pPGI-independent mechanisms.