Open Access
In vitro evaluation of postbiotics produced from bacterial isolates obtained from rainbow trout and nile tilapia against the pathogens Yersinia ruckeri and Aeromonas salmonicida subsp. Salmonicida
(MDPI, 2023) Quintanilla Pineda, Mario Alejandro; Garrote Achou, Chajira Camila; Díaz, Jesús Vicente; Gutiérrez-Falcón, Ana; Bravo, María; Herrera-Muñoz, Juan Ignacio; Peña-Navarro, Nelson; Alvarado, Carlos; Ibáñez Moya, Francisco C.; Marzo Pérez, Florencio; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Ciencias; Zientziak
The use of antibiotics in aquaculture leads to the proliferation of multidrug-resistant bacteria, and an urgent need for developing new alternatives to prevent and control disease has, thus, arisen. In this scenario, postbiotics represent a promising tool to achieve this purpose; thus, in this study, isolation and selection of bacteria to further produce and evaluate their postbiotics antibacterial activity against fish pathogens was executed. In this respect, bacterial isolates from rainbow trout and Nile tilapia were obtained and tested in vitro against Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida. From 369 obtained isolates, 69 were selected after initial evaluation. Afterwards, additional screening was carried out by spot-on-lawn assay to finally select twelve isolates; four were identified as Pediococcus acidilactici, seven as Weissella cibaria, and one as Weissella paramesenteroides by matrix assisted laser desorption/ionization, time-of-flight mass spectrometry (MALDI-TOF MS). Selected bacteria were used to obtain postbiotic products to test their antagonistic activity through coculture challenge and broth microdilution assays. The influence of incubation time prior to postbiotic production on antagonistic behavior was also recorded. Two isolates identified as W. cibaria were able to significantly reduce (p < 0.05) A. salmonicida subsp. salmonicida’s growth in the coculture challenge up to 4.49 ± 0.05 Log CFU/mL, and even though the reduction in Y. ruckeri was not as effective, some inhibition on the pathogen’s growth was reported; at the same time, most of the postbiotic products obtained showed more antibacterial activity when obtained from broth cultures incubated for 72 h. Based on the results obtained, the preliminary identification of the isolates that expressed the highest inhibitory activity was confirmed by partial sequencing as W. cibaria. Through our study, it can be concluded that postbiotics produced by these strains are useful to inhibit the growth of the pathogens and could, thereby, be applicable in further research to develop suitable tools as feed additives for disease control and prevention in aquaculture.
Open Access
Modeling the growth of six Listeria monocytogenes strains in smoked salmon pâté
(MDPI, 2023) Bolívar, Araceli; Garrote Achou, Chajira Camila; Tarlak, Fatih; Cantalejo Díez, María Jesús; Correia Peres Costa, Jean Carlos; Pérez Rodríguez, Fernando; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura
In this study, the growth of six L. monocytogenes strains isolated from different fish products was quantified and modeled in smoked salmon pâté at a temperature ranging from 2 to 20 °C. The experimental data obtained for each strain was fitted to the primary growth model of Baranyi and Roberts to estimate the following kinetic parameters: lag phase (λ), maximum specific growth rate (μmax), and maximum cell density (Nmax). Then, the effect of storage temperature on the obtained μmax values was modeled by the Ratkowsky secondary model. In general, the six L. monocytogenes strains showed rapid growth in salmon pâté at all storage temperatures, with a relatively short lag phase λ, even at 2 °C. The growth behavior among the tested strains was similar at the same storage temperature, although significant differences were found for the parameters λ and μmax. Besides, the growth variations among the strains did not follow a regular pattern. The estimated secondary model parameter Tmin ranged from −4.25 to −3.19 °C. This study provides accurate predictive models for the growth of L. monocytogenes in fish pâtés that can be used in shelf life and microbial risk assessment studies. In addition, the models generated in this work can be implemented in predictive modeling tools and repositories that can be reliably and easily used by the fish industry and end-users to establish measures aimed at controlling the growth of L. monocytogenes in fish-based pâtés.
Open Access
Evaluation of different nutritional sources in lactic acid bacteria fermentation for sustainable postbiotic production
(MDPI, 2025-02-14) Garrote Achou, Chajira Camila; Cantalejo Díez, María Jesús; Díaz Cano, Jesús; Molinos Equiza, Xabier ; Institute on Innovation and Sustainable Development in Food Chain - ISFOOD; Gobierno de Navarra / Nafarroako Gobernua
In recent years, interest in postbiotics has grown due to their potential health benefits and applications in food systems. This study evaluated various nutritional sources for lactic acid bacteria (LAB) fermentation to enhance postbiotic production. Three LAB strains were tested: Pediococcus acidilactici CECT 9879 (PA), Weissella cibaria CECT 30731 (WC), and Lactococcus lactis CECT 30734 (LL). Fermentation experiments assessed bacterial growth, pH levels, and antibacterial activity against E. coli using different carbon and nitrogen sources. Fructose and xylose significantly improved growth in WC (9.39 ± 0.16 log CFU/mL) and LL (9.37 ± 0.22 log CFU/mL) compared to glucose. Ribose enhanced antimicrobial activity in PA (41.67 ± 2.89%) and WC (50.00 ± 0.00%) relative to glucose. Additionally, plant-based nitrogen sources, such as soy (LL: 8.93 ± 0.12 log CFU/mL and 81.67 ± 2.89%) and wheat (WC: 9.40 ± 0.17 log CFU/mL and 65.00 ± 0.00%), along with microbial sources like yeast (PA: 9.57 ± 0.12 log CFU/mL and 40.00 ± 0.00%), effectively supported growth and antibacterial activity. These findings highlight the potential of developing animal-free fermentation media that meet nutritional, safety, and sustainability criteria while making a significant contribution to the optimization of postbiotic production.
Open Access
A novel postbiotic product based on Weissella cibaria for enhancing disease resistance in rainbow trout: aquaculture application
(MDPI, 2024) Quintanilla Pineda, Mario Alejandro; Ibáñez Moya, Francisco C.; Garrote Achou, Chajira Camila; Marzo Pérez, Florencio; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Ciencias; Zientziak
Postbiotics are innovative tools in animal husbandry, providing eco-friendly solutions for disease management within the industry. In this study, a new postbiotic product was evaluated for its impact on the health of rainbow trout (Oncorhynchus mykiss). In vivo studies were conducted to assess the safety of the Weissella cibaria strains used in postbiotic production. Additionally, this study evaluated the impact of diet supplementation with 0.50% postbiotics on growth performance during a 30-day feeding trial; the gut microbial communities, immunomodulation, and protection against Yersinia ruckeri infection were evaluated. The strains did not harm the animals during the 20-day observation period. Furthermore, the effect of postbiotics on growth performance was not significant (p < 0.05). The treated group showed a significant increase in acid-lactic bacteria on the 30th day of the feeding trial, with counts of 3.42 ± 0.21 log CFU/mL. Additionally, there was an up-regulation of the pro-inflammatory cytokine IL-1β in head kidney samples after 48 h of feed supplementation, whereas cytokines IL-10, IL-8, INF-γ, and TNF-α were down-regulated. The findings indicate that rainbow trout fed with postbiotics saw an improvement in their survival rate against Y. ruckeri, with a 20.66% survival improvement in the treated group. This study proves that incorporating postbiotics from two strains of W. cibaria previously isolated from rainbow trout into the diet of fish has immunomodulatory effects, enhances intestinal microbial composition, and improves fish resistance against Y. ruckeri.

Garrote Achou, Chajira Camila

Email Address

person.page.identifierURI

Birth Date

Job Title

Last Name

First Name

person.page.departamento

person.page.instituteName

ORCID

person.page.observainves

person.page.upna

Name

Filters

Author

Subject

Date

Has files

Item Type

Type

Settings

Sort By

Results per page

Search Results