Person: Pablo Maiso, Lorena de
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Pablo Maiso
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Lorena de
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Instituto de Agrobiotecnología (IdAB)
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0000-0003-3273-591X
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810712
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Publication Open Access Lentinula edodes β-glucan enriched diet induces pro- and anti-inflammatory macrophages in rabbit(Taylor & Francis, 2017) Crespo Otano, Helena; Guillén, Hugo; Pablo Maiso, Lorena de; Gómez Arrebola, Carmen; Rodríguez, Gregorio; Glaría Ezquer, Idoia; Andrés Cara, Damián de; Reina Arias, Ramsés; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaβ-glucans exhibited in cell walls of several pathogens as bacteria or fungi are sensed by pathogen recognition receptors such as scavenger receptors present in antigen presenting cells, i.e., macrophages. β-glucans obtained from Shiitake mushrooms were chemically characterized. A β-glucan supplemented diet was assayed for 30 days in rabbits aiming to characterize the immune response elicited in blood-derived macrophages. M1 and M2 profiles of macrophage differentiation were confirmed in rabbits by in vitro stimulation with IFN-γ and IL-4 and marker quantification of each differentiation pathway. Blood derived macrophages from rabbits administered in vivo with the β-glucan supplemented diet showed higher IL-4, IFN-γ and RAGE together with lower IL-10 relative expression, indicative of an ongoing immune response. Differences in IL-1β, IL-13 and IL-4 expression were also found in rabbit sera by ELISA suggesting further stimulation of the adaptive response. Recent challenges in the rabbit industry include the search of diet supplements able to elicit an immune stimulation with particular interest in facing pathogens such as viruses or bacteria. β–glucans from fungi may contribute to maintain an immune steady state favouring protection and thus reducing antibiotic treatment.Publication Open Access Multi-platform detection of small ruminant lentivirus antibodies and provirus as biomarkers of production losses(Frontiers Media, 2020) Echeverría Garín, Irache; Miguel, Ricardo de; Pablo Maiso, Lorena de; Glaría Ezquer, Idoia; Benito, Alfredo A.; Blas, Ignacio de; Andrés Cara, Damián de; Luján, Lluís; Reina Arias, Ramsés; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta ElikaduraSmall ruminant lentiviruses (SRLVs) are endemic in most areas of Europe, causing a chronic infection and a multisystemic disease affecting the udder, carpal joints, lungs, and central nervous system. Due to the lack of treatments and protective vaccination strategies, infection control is focused on the identification of infected animals through serological or molecular techniques. However, antigenic and genetic heterogeneity of SRLVs represent a clear drawback for diagnosis. Infected animals may present lower animal production parameters such as birth weight or milk production and quality, depending on productive systems considered and, likely, to the diagnostic method applied. In this study, four sheep flocks dedicated to dairy or meat production were evaluated using three different ELISA and two PCR strategies to classify animal population according to SRLV infection status. Productive parameters were recorded along one whole lactation or reproductive period and compared between positive and negative animals. SRLV was present in 19% of the total population, being unequally distributed in the different flocks. Less than half of the infected animals were detected by a single diagnostic method, highlighting the importance of combining different diagnostic techniques. Statistical analysis employing animal classification using all the diagnostic methods associated lambing size, lamb weight at birth, and daily weight gain with SRLV infection status in meat flocks. Milk production, somatic cell count, fat, and protein content in the milk were associated with SRLV infection in dairy flocks, to a greater extent in the flock showing higher seroprevalence. A multi-platform SRLV diagnostic strategy was useful for ensuring correct animal classification, thus validating downstream studies investigating production traits.Publication Open Access Nuevos avances para el desarrollo de vacunas frente a lentivirus(Interempresasmedia, 2020) Reina Arias, Ramsés; Nistal Villán, Estanislao; Pablo Maiso, Lorena de; Echeverría Garín, Irache; Glaría Ezquer, Idoia; Andrés Cara, Damián de; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta ElikaduraLa infección de células ovinas por un virus murino no patogénico es capaz de interferir en la replicación de virus patogénicos como el Maedi Visna. El éxito en la defensa está condicionado por la capacidad inicial de respuesta con la que cuentan casi todas las células de los organismos vivos, participando en la inmunidad innata frente a cualquier infección.Publication Open Access Characterization of ovine A3Z1 restriction properties against small ruminant lentiviruses (SRLVs)(MDPI, 2017) Pablo Maiso, Lorena de; Glaría Ezquer, Idoia; Crespo Otano, Helena; Nistal Villán, Estanislao; Andrésdóttir, Valgerdur; Andrés Cara, Damián de; Amorena Zabalza, Beatriz; Reina Arias, Ramsés; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua; Gobierno de Navarra / Nafarroako Gobernua; Universidad Pública de Navarra / Nafarroako Unibertsitate PublikoaIntrinsic factors of the innate immune system include the apolipoprotein B editing enzyme catalytic polypeptide-like 3 (APOBEC3) protein family. APOBEC3 inhibits replication of different virus families by cytosine deamination of viral DNA and a not fully characterized cytosine deamination-independent mechanism. Sheep are susceptible to small ruminant lentivirus (SRLVs) infection and contain three APOBEC3 genes encoding four proteins (A3Z1, Z2, Z3 and Z2-Z3) with yet not deeply described antiviral properties. Using sheep blood monocytes and in vitro-derived macrophages, we found that A3Z1 expression is associated with lower viral replication in this cellular type. A3Z1 transcripts may also contain spliced variants (A3Z1Tr) lacking the cytidine deaminase motif. A3Z1 exogenous expression in fully permissive fibroblast-like cells restricted SRLVs infection while A3Z1Tr allowed infection. A3Z1Tr was induced after SRLVs infection or stimulation of blood-derived macrophages with interferon gamma (IFN- ). Interaction between truncated isoform and native A3Z1 protein was detected as well as incorporation of both proteins into virions. A3Z1 and A3Z1Tr interacted with SRLVs Vif, but this interaction was not associated with degradative properties. Similar A3Z1 truncated isoforms were also present in human and monkey cells suggesting a conserved alternative splicing regulation in primates. A3Z1-mediated retroviral restriction could be constrained by different means, including gene expression and specific alternative splicing regulation, leading to truncated protein isoforms lacking a cytidine-deaminase motif.