Publication:
Multi-platform detection of small ruminant lentivirus antibodies and provirus as biomarkers of production losses

Date

2020

Director

Publisher

Frontiers Media
Acceso abierto / Sarbide irekia
Artículo / Artikulua
Versión publicada / Argitaratu den bertsioa

Project identifier

AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096172-B-C31/ES/recolecta
Gobierno de Navarra//210160006

Abstract

Small ruminant lentiviruses (SRLVs) are endemic in most areas of Europe, causing a chronic infection and a multisystemic disease affecting the udder, carpal joints, lungs, and central nervous system. Due to the lack of treatments and protective vaccination strategies, infection control is focused on the identification of infected animals through serological or molecular techniques. However, antigenic and genetic heterogeneity of SRLVs represent a clear drawback for diagnosis. Infected animals may present lower animal production parameters such as birth weight or milk production and quality, depending on productive systems considered and, likely, to the diagnostic method applied. In this study, four sheep flocks dedicated to dairy or meat production were evaluated using three different ELISA and two PCR strategies to classify animal population according to SRLV infection status. Productive parameters were recorded along one whole lactation or reproductive period and compared between positive and negative animals. SRLV was present in 19% of the total population, being unequally distributed in the different flocks. Less than half of the infected animals were detected by a single diagnostic method, highlighting the importance of combining different diagnostic techniques. Statistical analysis employing animal classification using all the diagnostic methods associated lambing size, lamb weight at birth, and daily weight gain with SRLV infection status in meat flocks. Milk production, somatic cell count, fat, and protein content in the milk were associated with SRLV infection in dairy flocks, to a greater extent in the flock showing higher seroprevalence. A multi-platform SRLV diagnostic strategy was useful for ensuring correct animal classification, thus validating downstream studies investigating production traits.

Description

Keywords

Small ruminant lentiviruses, Diagnosis, Milk production, Somatic cell count, Body weight, Lambing size, ELISA, PCR

Department

Agronomía, Biotecnología y Alimentación / Agronomia, Bioteknologia eta Elikadura

Faculty/School

Degree

Doctorate program

item.page.cita

Echeverría, I., De Miguel, R., De Pablo-Maiso, L., Glaria, I., Benito, A. A., De Blas, I., De Andrés, D., Luján, L., Reina, R. (2020) Multi-platform detection of small ruminant lentivirus antibodies and provirus as biomarkers of production losses. Frontiers in Veterinary Science, 7, 1-10. https://doi.org/10.3389/fvets.2020.00182.

item.page.rights

© 2020 Echeverría, De Miguel, De Pablo-Maiso, Glaria, Benito, De Blas, De Andrés, Luján and Reina. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).

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