Zubiate Orzanco, Pablo
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Zubiate Orzanco
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Pablo
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Ingeniería Eléctrica, Electrónica y de Comunicación
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Publication Open Access Lossy mode resonance sensors based on nanocoated multimode-coreless-multimode fibre(Elsevier, 2020) Vicente Gómara, Adrián; Santano Rivero, Desiree; Zubiate Orzanco, Pablo; Urrutia Azcona, Aitor; Del Villar, Ignacio; Ruiz Zamarreño, Carlos; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Institute of Smart Cities - ISC; Ingeniería Eléctrica, Electrónica y de Comunicación; Gobierno de Navarra / Nafarroako Gobernua; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, PJUPNA26In this work it is proved the ability to obtain lossy mode resonances (LMRs) in the transmission spectrum with multimode-coreless-multimode fibre optic structure coated with tin oxide on the coreless segment. The devices were characterized as a function of the surrounding medium refractive index and sensitivities of 7346.93 nm/RIU and 708.57 nm/RIU were attained for the first and the second LMR respectively. As an application proof of this technology, one of the devices was biofunctionalized and used for detecting goat anti-mouse IgG in concentrations ranging from 1 to 40 mg/L, with a limit of detection of 0.6 mg/L. This proves the ability of this simple structure to be used for biological, chemical or environmental applications.Publication Open Access Albumin-based optical and electrochemical biosensors for PFAS detection: a comparison(Springer, 2023) Moro, G.; Chiavaioli, Francesco; Zubiate Orzanco, Pablo; Del Villar, Ignacio; Baldini, Francesco; De Wael, K.; Moretto, L. M.; Giannetti, Ambra; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza; Institute of Smart Cities - ISCThe widespread industrial use of per- and polyfluoroalkyl substances (PFAS) have engendered the release of these manmade chemicals in the environment with harmful effects on animal and human health. To monitor PFAS levels in drinking waters, sensitive and versatile sensing strategies are urgently required. Since many perfluoroalkyl carboxylic acids, such as perfluorooctanoic acid (PFOA), are fatty acid-mimic, delipidated human serum albumin (HSA) can be applied as biorecognition element for the design of novel PFAS sensors. Here, two albumin-based biosensing strategies are described and compared: i) a lossy mode resonance (LMR) fiber optic one and ii) an impedimetric portable one developed on screen-printed electrodes. In both biosensing platforms, HSA was covalently immobilized via EDC/NHS chemistry using the carboxylic moieties of the polymeric layers previously deposited at the transducer surface. Afterwards, the conformational changes related to the formation of HSA/PFOA complex were followed considering: i) the LMR spectral shifts for the optical platform and ii) the changes of absolute impedance for the impedimetric one. The performance and future developments of both PFOA biosensors are discussed.