Open Access
Lossy mode resonance sensors based on nanocoated multimode-coreless-multimode fibre
(Elsevier, 2020) Vicente Gómara, Adrián; Santano Rivero, Desiree; Zubiate Orzanco, Pablo; Urrutia Azcona, Aitor; Del Villar, Ignacio; Ruiz Zamarreño, Carlos; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Institute of Smart Cities - ISC; Ingeniería Eléctrica, Electrónica y de Comunicación; Gobierno de Navarra / Nafarroako Gobernua; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, PJUPNA26
In this work it is proved the ability to obtain lossy mode resonances (LMRs) in the transmission spectrum with multimode-coreless-multimode fibre optic structure coated with tin oxide on the coreless segment. The devices were characterized as a function of the surrounding medium refractive index and sensitivities of 7346.93 nm/RIU and 708.57 nm/RIU were attained for the first and the second LMR respectively. As an application proof of this technology, one of the devices was biofunctionalized and used for detecting goat anti-mouse IgG in concentrations ranging from 1 to 40 mg/L, with a limit of detection of 0.6 mg/L. This proves the ability of this simple structure to be used for biological, chemical or environmental applications.
Open Access
Lossy mode resonance-based optical immunosensor towards detecting gliadin in aqueous solutions
(Elsevier, 2023) Benítez Pérez, Melanys; Zubiate Orzanco, Pablo; Socorro Leránoz, Abián Bentor; Matías Maestro, Ignacio; Ingeniería Eléctrica, Electrónica y de Comunicación; Institute of Smart Cities - ISC; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
The development of accurate, intuitive, and easy-to-handle devices to detect different types of allergens is on the rise, as these are useful tools to guarantee consumer safety, which should be a priority for any food industry. Gliadin, one of the main proteins present in gluten, is the one responsible for triggering the immune system to produce autoantibodies in celiac disease, the most dangerous pathology related to gluten. Lossy Mode Resonance (LMR) based biosensors are lately known as a promising sensing technology and its implementation on planar waveguides has been shown to result in manageable, sustainable and robust structures. In this work, an LMR based microfluidic biosensor for gliadin detection is proposed, by coating a coverslip with Titanium Dioxide (TiO2) by Atomic Layer Deposition (ALD) to generate the resonance phenomena and functionalizing the sensor surface with anti-gliadin antibody (AGA) through covalent bond. The sensor was exposed to different gliadin concentrations in ultrapure water, in the range of 0.1–100 ppm with an accuracy of ±0.14 ppm, for a sensitivity of 1.35 ppm/ml. The calibration curve was obtained from the experimental data corresponding to three repetitions of the assay and a limit of detection (LOD) of 0.05 ppm was achieved. Moreover, the sensor was exposed to commercial flour samples, some of them labeled as gluten free (GF) and the response agreed with the expected results according to product label. Biosensor specificity to gliadin was demonstrated by injecting chicken egg white albumin without obtaining any significant response.
Open Access
Albumin-based optical and electrochemical biosensors for PFAS detection: a comparison
(Springer, 2023) Moro, G.; Chiavaioli, Francesco; Zubiate Orzanco, Pablo; Del Villar, Ignacio; Baldini, Francesco; De Wael, K.; Moretto, L. M.; Giannetti, Ambra; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza; Institute of Smart Cities - ISC
The widespread industrial use of per- and polyfluoroalkyl substances (PFAS) have engendered the release of these manmade chemicals in the environment with harmful effects on animal and human health. To monitor PFAS levels in drinking waters, sensitive and versatile sensing strategies are urgently required. Since many perfluoroalkyl carboxylic acids, such as perfluorooctanoic acid (PFOA), are fatty acid-mimic, delipidated human serum albumin (HSA) can be applied as biorecognition element for the design of novel PFAS sensors. Here, two albumin-based biosensing strategies are described and compared: i) a lossy mode resonance (LMR) fiber optic one and ii) an impedimetric portable one developed on screen-printed electrodes. In both biosensing platforms, HSA was covalently immobilized via EDC/NHS chemistry using the carboxylic moieties of the polymeric layers previously deposited at the transducer surface. Afterwards, the conformational changes related to the formation of HSA/PFOA complex were followed considering: i) the LMR spectral shifts for the optical platform and ii) the changes of absolute impedance for the impedimetric one. The performance and future developments of both PFOA biosensors are discussed.

Zubiate Orzanco, Pablo

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