García Ona, Enrique
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García Ona
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Enrique
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Instituto de Agrobiotecnología (IdAB)
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Publication Restricted Evaluación de guantes de protección para la poda con tijeras neumáticas y eléctricas(2008) García Ona, Enrique; Arnal Atarés, Pedro; Escuela Técnica Superior de Ingenieros Agrónomos; Nekazaritza Ingeniarien Goi Mailako Eskola Teknikoa; Proyectos e Ingeniería Rural; Landa Ingeniaritza eta ProiektuakPublication Open Access Análisis de una cepa avirulenta de Salmonella enteritidis para su uso como vacuna viva atenuada(2011) García Ona, Enrique; Solano Goñi, Cristina; Escuela Técnica Superior de Ingenieros Agrónomos; Nekazaritza Ingeniarien Goi Mailako Eskola TeknikoaSalmonella enterica subs. enterica ser. Enteritidis es un importante patógeno zoonótico con capacidad de infectar a un amplio rango de huéspedes. Actualmente representa un importante problema de salud pública dado que en el año 2010 se declararon en Europa 100.000 casos de enterocolitis por esta bacteria (de los cuales 2500 casos fueron declarados en España), asociados principalmente al consumo de carne de origen porcino. Para reducir la prevalencia de Salmonella en las granjas, la Directiva (CE) 2160/2003 de la Unión Europea propone exigir la vacunación de toda la cabaña ganadera avícola y porcina. Para responder a esta necesidad, en este estudio hemos valorado la capacidad de una cepa atenuada de S. Enteritidis para el desarrollo de una vacuna capaz de inducir una respuesta inmune que disminuya la colonización de órganos y liberación de Salmonella en heces en explotaciones intensivas de ganado porcino. La cepa de Salmonella candidata para el desarrollo de la vacuna ha sido, S. Enteritidis 3934ΔXII*, que tiene delecionado los genes que codifican las 12 proteínas de la familia GGDEF. Esta cepa es avirulenta, tiene muy disminuida su capacidad para sobrevivir en el ambiente y como no contiene DNA exógeno no estaría catalogada como OGM. Ensayos de inmunización por vía oral utilizando un modelo de infección murino, revelaron que la inmunización con una sola dosis de 107ufc de esta cepa es capaz de inducir una respuesta inmune protectora frente a una infección por Salmonella, indicando que dicha cepa puede ser un candidato idóneo para su uso como vacuna viva atenuada.Publication Open Access Evaluation of a Salmonella strain lacking the secondary messenger c-di-GMP and RpoS as a live oral vaccine(Public Library of Science, 2016) Latasa Osta, Cristina; Echeverz Sarasúa, Maite; García Ona, Enrique; Burgui Erice, Saioa; Casares, Noelia; Hervás Stubbs, Sandra; Lasarte, Juan José; Lasa Uzcudun, Íñigo; Solano Goñi, Cristina; García Martínez, Begoña; Gil Puig, Carmen; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua; Gobierno de Navarra / Nafarroako Gobernua: IIM 13329.RI1Salmonellosis is one of the most important bacterial zoonotic diseases transmitted through the consumption of contaminated food, with chicken and pig related products being key reservoirs of infection. Although numerous studies on animal vaccination have been performed in order to reduce Salmonella prevalence, there is still a need for an ideal vaccine. Here, with the aim of constructing a novel live attenuated Salmonella vaccine candidate, we firstly analyzed the impact of the absence of cyclic-di-GMP (c-di-GMP) in Salmonella virulence. Cdi-GMP is an intracellular second messenger that controls a wide range of bacterial processes, including biofilm formation and synthesis of virulence factors, and also modulates the host innate immune response. Our results showed that a Salmonella multiple mutant in the twelve genes encoding diguanylate cyclase proteins that, as a consequence, cannot synthesize c-di-GMP, presents a moderate attenuation in a systemic murine infection model. An additional mutation of the rpoS gene resulted in a synergic attenuating effect that led to a highly attenuated strain, referred to as ΔXIII, immunogenic enough to protect mice against a lethal oral challenge of a S. Typhimurium virulent strain. ΔXIII immunogenicity relied on activation of both antibody and cell mediated immune responses characterized by the production of opsonizing antibodies and the induction of significant levels of IFN-γ, TNF- α, IL-2, IL-17 and IL-10. ΔXIII was unable to form a biofilm and did not survive under desiccation conditions, indicating that it could be easily eliminated from the environment. Moreover, ΔXIII shows DIVA features that allow differentiation of infected and vaccinated animals. Altogether, these results show ΔXIII as a safe and effective live DIVA vaccinePublication Open Access A DIVA vaccine strain lacking RpoS and the secondary messenger c-di-GMP for protection against salmonellosis in pigs(BioMed Central, 2020) Gil Puig, Carmen; Latasa Osta, Cristina; García Ona, Enrique; Lázaro, Isidro; Labairu, Javier; Echeverz Sarasúa, Maite; Burgui Erice, Saioa; García Martínez, Begoña; Lasa Uzcudun, Íñigo; Solano Goñi, Cristina; Ciencias de la Salud; Osasun Zientziak; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa; Gobierno de Navarra / Nafarroako Gobernua, IIM 13329.RI1Salmonellosis is the second most common food-borne zoonosis in the European Union, with pigs being a major reservoir of this pathogen. Salmonella control in pig production requires multiple measures amongst which vaccination may be used to reduce subclinical carriage and shedding of prevalent serovars, such as Salmonella enterica serovar Typhimurium. Live attenuated vaccine strains offer advantages in terms of enhancing cell mediated immunity and allowing inoculation by the oral route. However, main failures of these vaccines are the limited cross-protection achieved against heterologous serovars and interference with serological monitoring for infection. We have recently shown that an attenuated S. Enteritidis strain (ΔXIII) is protective against S. Typhimurium in a murine infection model. ΔXIII strain harbours 13 chromosomal deletions that make it unable to produce the sigma factor RpoS and synthesize cyclic-di-GMP (c-di-GMP). In this study, our objectives were to test the protective effects of ΔXIII strain in swine and to investigate if the use of ΔXIII permits the discrimination of vaccinated from infected pigs. Results show that oral vaccination of pre-weaned piglets with ΔXIII cross-protected against a challenge with S. Typhimurium by reducing faecal shedding and ileocaecal lymph nodes colonization, both at the time of weaning and slaughter. Vaccinated pigs showed neither faecal shedding nor tissue persistence of the vaccine strain at weaning, ensuring the absence of ΔXIII strain by the time of slaughter. Moreover, lack of the SEN4316 protein in ΔXIII strain allowed the development of a serological test that enabled the differentiation of infected from vaccinated animals (DIVA).