Open Access
Evaluación del interés de métodos sencillos de medida de la actividad microbiana del suelo para la identificación y caracterización de terroirs vitícolas
(2020) Loidi Erviti, Maite; Santesteban García, Gonzaga; Abad Zamora, Francisco Javier; Escuela Técnica Superior de Ingeniería Agronómica y Biociencias; Nekazaritzako Ingeniaritzako eta Biozientzietako Goi Mailako Eskola Teknikoa
En la viticultura, la actividad biológica del suelo es uno de los factores biológicos, que junto a los antrópicos y a los físicos determina la identidad del vino de una bodega o terroir. Sin embargo, no se dispone de métodos que permitan, de manera sencilla, estimar esta actividad. El objetivo de este trabajo es evaluar si el método TBI (tea bag index) y el uso de placas Biolog EcoplatesTM permiten diferenciar entre muestras procedentes de suelos de una explotación vitícola situada en Traibuenas (Navarra) en función de la zona de muestreo, de la profundidad a la que se toma la muestra y de si las muestras se toman en la línea del cultivo o en las calles. Además, se evaluó si las diferencias observadas se corresponden con los terroirs definidos en el viñedo por el personal técnico de la bodega propietaria. A la vista de los resultados, los índices empleados se consideran de interés, siendo el índice de Shannon medido a través del método Biolog EcoplatesTM el que mejor permitió diferenciar los terroirs. En cuanto a los parámetros de muestreo, la profundidad afecta más a los parámetros del TBI que a los del método Biolog EcoplatesTM, siendo relevante para todos los índices la época del ciclo en las que se realizó el muestreo.
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Efecto del aclareo de racimos y de puntas sobre la producción y la calidad de la uva en Vitis vinifera L. cv. Tempranillo
(2008) Loidi Erviti, Maite; Santesteban García, Gonzaga; Escuela Técnica Superior de Ingenieros Agrónomos; Nekazaritza Ingeniarien Goi Mailako Eskola Teknikoa; Producción Agraria; Nekazaritza Ekoizpena
Open Access
Evaluation of the incidence of severe trimming on grapevine (Vitis vinifera L.) water consumption
(Elsevier, 2019-03-01) Abad Zamora, Francisco Javier; Marín Ederra, Diana; Loidi Erviti, Maite; Miranda Jiménez, Carlos; Royo Díaz, José Bernardo; Urrestarazu Vidart, Jorge; Santesteban García, Gonzaga; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute for Multidisciplinary Research in Applied Biology - IMAB; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, FPI-UPNA-2016; Gobierno de Navarra / Nafarroako Gobernua
Viticulture in Southern Europe heads towards a scenario of drier and warmer the growing seasons due to climate change. This decrease in the amount of water available for the vines and increase in evapotranspiration make necessary finding strategies to reduce vineyard water needs. In this context, the effect of severe trimming (40¿60% of shoot length), performed at pea-size stage, on plant water status was evaluated in four different vineyards located in North of Spain. Severe trimming improved plant water status clearly only when climate conditions were more demanding, whereas only a slight improvement or no change was observed elsewhere. Lower leaf areas resulted in less water deficit following a logarithmical trend revealing that the effect was more pronounced at low leaf area levels. Severe trimming had non-significant effects on cluster number, yield and cluster weight, but presents a tendency to reduce total soluble solids content and to increase total acidity, consequently delaying ripening.
Open Access
Unlocking Spanish pear genetic diversity: strategies for construction of a national core collection
(Springer Nature, 2024-11-04) Irisarri, Patricia; Urrestarazu Vidart, Jorge; Ramos-Cabrer, Ana; Pereira-Lorenzo, Santiago; Velázquez-Barrera, María Encarnación; Díaz-Hernández, María Belén; Dapena, Enrique; Urbina, Valero; Dalmases, Josep; Ríos-Mesa, Domingo; Crespo Martínez, Sara; Loidi Erviti, Maite; Santesteban García, Gonzaga; Ascasíbar-Errasti, Javier; Errea, Pilar; Miranda Jiménez, Carlos; Pina, Ana; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute for Multidisciplinary Research in Applied Biology - IMAB
Spanish pear germplasm collections are crucial for preservation, research, and breeding efforts. However, genetic diversity and structure is unknown at national level. A coordinated national project analyzed 1251 accessions from 7 Spanish pear collections using an internationally recognized set of 14 SSRs to enhance the utilization of these collections. Key findings included the identification of 760 unique genotypes (490 diploids and 270 triploids). Notably, genotypes represented by a single accession accounted for 49% of the total, indicating high vulnerability of this material. Using a Bayesian clustering method revealed two main genetic groups, G1 containing most foreign cultivars and G2 retaining local Spanish cultivars, which were further divided into two other subgroups using a nested approach, revealing moderate but significant differentiation among them. The populations were renamed according to the origin of the reference samples assigned to each group as 'South' (G1.1), 'Western Europe-1' (G1.2), 'Western Europe-2' (G2.1) and 'No-Pyrus communis' (G2.2). The results led to the creation of a 'generalist' collection, aiming to maximize genetic diversity representativeness, starting with 68 genotypes but expanding to 111 to achieve better allele recovery. This core collection is a valuable resource for genetic studies and conservation, enhancing efforts to preserve pear biodiversity.
Open Access
Monitoring vineyard canopy management operations using UAV-acquired photogrammetric point clouds
(MDPI, 2020) López Granados, Francisca; Oneka Mugica, Oihane; Marín Ederra, Diana; Loidi Erviti, Maite; Santesteban García, Gonzaga; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa; Gobierno de Navarra / Nafarroako Gobernua
Canopy management operations, such as shoot thinning, leaf removal, and shoot trimming, are among the most relevant agricultural practices in viticulture. However, the supervision of these tasks demands a visual inspection of the whole vineyard, which is time-consuming and laborious. The application of photogrammetric techniques to images acquired with an Unmanned Aerial Vehicle (UAV) has proved to be an efficient way to measure woody crops canopy. Consequently, the objective of this work was to determine whether the use ofUAV photogrammetry allows the detection of canopy management operations. A UAV equipped with an RGB digital camera was used to acquire images with high overlap over different canopy management experiments in four vineyards with the aim of characterizing vine dimensions before and after shoot thinning, leaf removal, and shoot trimming operations. The images were processed to generate photogrammetric point clouds of every vine that were analyzed using a fully automated object-based image analysis algorithm. Two approaches were tested in the analysis of the UAV derived data: (1) to determine whether the comparison of the vine dimensions before and after the treatments allowed the detection of the canopy management operations; and (2) to study the vine dimensions after the operations and assess the possibility of detecting these operations using only the data from the flight after them. The first approach successfully detected the canopy management. Regarding the second approach, significant differences in the vine dimensions after the treatments were detected in all the experiments, and the vines under the shoot trimming treatment could be easily and accurately detected based on a fixed threshold.
Open Access
Timing of defoliation affects anthocyanin and sugar decoupling in Grenache variety growing in warm seasons
(Elsevier, 2024) Fernández-Zurbano, Purificación; Santesteban García, Gonzaga; Villa Llop, Ana; Loidi Erviti, Maite; Peñalosa, Carlos; Músquiz, Sergio; Torres Molina, Nazareth; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute for Multidisciplinary Research in Applied Biology - IMAB; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Warming trends over the winegrowing regions lead to an advance of grapevine phenology, decreased yield and increased sugar content with a lower polyphenol content. We hypothesized that different leaf removal timings may counteract these effects. A two-year experiment was conducted in La Rioja (Spain) with Vitis vinifera L. cv. Grenache trained in an open-vase system. Trial consisted in a complete block design with two leaf removal treatments differing in the moment of manipulation: i) severe leaf removal treatment conducted after fruit set (ELR); and ii) severe leaf removal at veraison (LLR) compared to an untreated control (Control). Both leaf removal treatments tended to decrease sugar content with no effect on yield, these effects being highly affected by the year. Defoliation accounted for a decreased flavanol and stilbene contents in berries at harvest. An ELR increased anthocyanin and phenolic acid contents at harvest, while warming during 2022 accounted for decreased contents of all the monitored groups of flavonols. ELR was only effective for delaying ripening by means of impairing the sugar:anthocyanin decoupling during the 2021 growing season which was related to lower % of kaempferol. Altogether, results suggested that defoliation should still be applied under currently warming trends in some viticulture regions.
Open Access
Intracultivar genetic diversity in grapevine: water use efficiency variability within cv. Grenache
(Wiley, 2021) Buesa, Ignacio; Escalona, José M.; Tortosa, Ignacio; Marín Ederra, Diana; Loidi Erviti, Maite; Santesteban García, Gonzaga; Douthe, Cyril; Medrano, H.; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura
The selection of genotypes best adapted to environmental conditions has traditionally focused on agronomic and grape composition parameters. However, to classify the genotypes most adapted to climate change conditions, the aim must be to focus on the ecophysiological responses that will ultimately determine their performance. The variability in water use efficiency of 13 Grenache genotypes over three-seasons was assessed under field conditions at leaf, grape and plant level. Results showed a significant effect of genotype at all three levels, and despite the large interannual variability there was a remarkable consistency among levels. Furthermore, using genotype-specific regressions it was possible to identify significant differences in the intrinsic water use efficiency response of each genotype as a function of the vine water status. The relationship between net photosynthesis and stomatal conductance, as well as carbon isotope discrimination in grapes, were also confirmed as reliable physiological indicators for selecting grapevine genotypes to future environmental conditions. Therefore, the proposed multi-level methodology was useful to quantify the intracultivar variability and the identification of more and less efficient genotypes within Grenache.
Open Access
Respectful pruning improves grapevine development: a case study in young vineyards
(Wiley, 2024-09-09) Galar Martínez, Mónica; Torres Molina, Nazareth; Sebastián Caumel, Bárbara; Palacios Muruzábal, Julián; Arzoz Lafuente, Ignacio; Juanena Ayestarán, Nahiara; Villa Llop, Ana; Loidi Erviti, Maite; Dewasme, Coralie; Roby, Jean Philippe; Santesteban García, Gonzaga; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute for Multidisciplinary Research in Applied Biology - IMAB; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Grapevine pruning is an agronomical operation required to regulate growth and yield, improve fruit quality, and maintain plant health and vigour. However, the intensification of the crop in the last decades, driven by efforts to increase productivity and decrease costs, has led to reduced attention to this operation. The aim of this study was to investigate whether different pruning styles during the first years of vineyard training affect grapevine development. Three pruning styles were followed in a newly planted cv. 'Tempranillo' vineyard for four consecutive pruning seasons: (i) respectful (RESP), where pruning cuts were always made leaving protection wood between the cut and vine perennial structures, and the preferential sap flow path was chosen; (ii) aggressive (AGGR), where no protection wood was left and, when possible, the nonpreferential sap flow path was followed; and (iii) winegrape grower (WIN), where the common practices used in the area were followed, which corresponds to an intermediate style between RESP and AGGR. In general, the RESP style increased the vegetative development compared to WIN and AGGR styles over the years and produced a higher yield in the first production year, although the intensity of the effect was not the same in the two vineyards considered. This was the first effort to systematically assess the effects of respectful pruning techniques on vine vegetative growth and demonstrate scientifically its impact.
Open Access
Nitrogen isotope ratio (δ15N): a nearly unexplored indicator that provides useful information in viticulture
(International Viticulture and Enology Society, 2024-05-15) Santesteban García, Gonzaga; Loidi Erviti, Maite; Urretavizcaya Sanz, Inés; Galar Martínez, Mónica; Crespo Martínez, Sara; Royo Díaz, José Bernardo; Miranda Jiménez, Carlos; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute for Multidisciplinary Research in Applied Biology - IMAB; Gobierno de Navarra / Nafarroako Gobernua
The study of the natural abundance of nitrogen-stable isotopes is an aspect of viticulture research that has received limited attention. While stable isotopes of carbon, oxygen and hydrogen have received significant attention, nitrogen isotope ratio (S15N) 15 N) remains a less studied yet potentially informative parameter. This paper explores the implications of S15N 15 N measurements in grapevines, shedding light on its utility as an indicator for nitrogen sources, plant water status, and within-field variability. The study examines the influence of nitrogen sources, dose, and sampling organs on S15N 15 N values, revealing distinct trends in petioles, berries, and seeds. Organic fertilisers led to higher S15N 15 N values compared to inorganic sources, while increasing nitrogen doses exhibited a much weaker effect on S15N. 15 N. Moreover, S15N 15 N values vary spatially within vineyards, associating with its topography and potential soil composition, soil management and water availability. Our results highlight the importance of considering S15N 15 N in viticulture, suggesting its integration with S13C 13 C for comprehensive insights into nitrogen cycling and soil management practices. The findings advocate for further research to harness the full potential of 15 N as a valuable tool in viticultural studies.
Open Access
Recovery of ancient grapevine plant material in peri‑urban areas. A case of success in Pamplona (Spain) leading to the recovery of cv. Berués
(Elsevier, 2022) Crespo Martínez, Sara; Mayor Azcona, Blanca; Oneka Mugica, Oihane; Loidi Erviti, Maite; Villa Llop, Ana; Marín Ederra, Diana; Miranda Jiménez, Carlos; Santesteban García, Gonzaga; Urrestarazu Vidart, Jorge; Agronomia, Bioteknologia eta Elikadura; Institute for Multidisciplinary Research in Applied Biology - IMAB; Agronomía, Biotecnología y Alimentación; Gobierno de Navarra / Nafarroako Gobernua
Viticulture was relatively important in the peri‑urban area of Pamplona till the end of the 19th century, but suffered a continued regression that has led to a nearly complete disappearance of vineyards. In this context, this work aims to evaluate the feasibility of recovering old grapevine germplasm in the peri‑urban area of Pamplona. The lack of a conventional source for recovering plant material (i.e. absence of old vineyards in the area) implied the need of designing an alternative prospecting procedure. This method included the analysis of the available historical information as open-access resources (orthophotos, land use maps and GIS applications) aiming to identify the areas with the highest probability of finding vines surviving from the general abandonment and uprooting of vineyards that had occurred in the 20th century. Based on the oldest on scale orthophoto available for Navarra, a vineyard land map of the peri‑urban area of Pamplona in year 1956 was built, allowing prospecting efforts to be focused on specific areas of primarily interest. Following this strategy, a total of 120 plants were collected, which corresponded to 44 genotypes. The most prominent achievement of this prospecting mission was the recovery of 15 accessions of Berués, a very old variety with a remarkable importance in the region according to old historical records, and considered to be disappeared. The methodology proposed was effective in searching for the oldest standing-alone plants surviving in the peri‑urban area of Pamplona, and may be adapted to assist the recovery of old grapevine germplasm in other currently non wine-growing regions/areas where viticulture was relevant some decades ago. © 2021