Person:
Aranjuelo Michelena, Iker

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https://academica-e.unavarra.es/handle/2454/48565

Last Name

Aranjuelo Michelena

First Name

Iker

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Ciencias

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0000-0002-8231-5043

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812253

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Now showing 1 - 10 of 30

Open Access
Differential effect of free-air CO2 enrichment (FACE) in different organs and growth stages of two cultivars of durum wheat
(MDPI, 2023) Gámez Guzmán, Angie Lorena; Han, Xue; Aranjuelo Michelena, Iker; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Ciencias; Zientziak
Wheat is a target crop within the food security context. The responses of wheat plants under elevated concentrations of CO2 (e[CO2]) have been previously studied; however, few of these studies have evaluated several organs at different phenological stages simultaneously under free-air CO2 enrichment (FACE) conditions. The main objective of this study was to evaluate the effect of e[CO2] in two cultivars of wheat (Triumph and Norin), analyzed at three phenological stages (elongation, anthesis, and maturation) and in different organs at each stage, under FACE conditions. Agronomic, biomass, physiological, and carbon (C) and nitrogen (N) dynamics were examined in both ambient CO2 (a[CO2]) fixed at 415 µmol mol−1 CO2 and e[CO2] at 550 µmol mol−1 CO2. We found minimal effect of e[CO2] compared to a[CO2] on agronomic and biomass parameters. Also, while exposure to 550 µmol mol−1 CO2 increased the photosynthetic rate of CO2 assimilation (An), the current study showed a diminishment in the maximum carboxylation (Vc,max) and maximum electron transport (Jmax) under e[CO2] conditions compared to a[CO2] at physiological level in both cultivars. However, even if no significant differences were detected between cultivars on photosynthetic machinery, differential responses between cultivars were detected in C and N dynamics at e[CO2]. Triumph showed starch accumulation in most organs during anthesis and maturation, but a decline in N content was observed. Contrastingly, in Norin, a decrease in starch content during the three stages and an increase in N content was observed. The amino acid content decreased in grain and shells at maturation in both cultivars, which might indicate a minimal translocation from source to sink organs. These results suggest a greater acclimation to e[CO2] enrichment in Triumph than Norin, because both the elongation stage and e[CO2] modified the source–sink relationship. According to the differences between cultivars, future studies should be performed to test genetic variation under FACE technology and explore the potential of cultivars to cope with projected climate scenarios.
Open Access
Multiscale assessment of ground, aerial and satellite spectral data for monitoring wheat grain nitrogen content
(Elsevier, 2022) Segarra, Joel; Rezzouk, Fatima Zahra; Aparicio, Nieves; González Torralba, Jon; Aranjuelo Michelena, Iker; Gracia-Romero, Adrian; Araus, José Luis; Kefauver, Shawn C.; Ciencias; Zientziak; Gobierno de Navarra / Nafarroako Unibertsitate Publikoa
Wheat grain quality characteristics have experienced increasing attention as a central factor affecting wheat end-use products quality and human health. Nonetheless, in the last decades a reduction in grain quality has been observed. Therefore, it is central to develop efficient quality-related phenotyping tools. In this sense, one of the most relevant wheat features related to grain quality traits is grain nitrogen content, which is directly linked to grain protein content and monitorable with remote sensing approaches. Moreover, the relation between nitrogen fertilization and grain nitrogen content (protein) plays a central role in the sustainability of agriculture. Both aiming to develop efficient phenotyping tools using remote sensing instruments and to advance towards a field-level efficient and sustainable monitoring of grain nitrogen status, this paper studies the efficacy of various sensors, multispectral and visible red–greenblue (RGB), at different scales, ground and unmanned aerial vehicle (UAV), and phenological stages (anthesis and grain filling) to estimate grain nitrogen content. Linear models were calculated using vegetation indices at each sensing level, sensor type and phenological stage. Furthermore, this study explores the up-scalability of the best performing model to satellite level Sentinel-2 equivalent data. We found that models built at the phenological stage of anthesis with UAV-level multispectral cameras using red-edge bands outperformed grain nitrogen content estimation (R2 = 0.42, RMSE = 0.18%) in comparison with those models built with RGB imagery at ground and aerial level, as well as with those built with widely used ground-level multispectral sensors. We also demonstrated the possibility to use UAV-built multispectral linear models at the satellite scale to determine grain nitrogen content effectively (R2 = 0.40, RMSE = 0.29%) at actual wheat fields.
Open Access
Additive effects of heatwave and water stresses on soybean seed yield is caused by impaired carbon assimilation at pod formation but not at flowering
(Elsevier, 2022) Soba Hidalgo, David; Arrese-Igor Sánchez, César; Aranjuelo Michelena, Iker; Institute for Multidisciplinary Research in Applied Biology - IMAB; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Heatwave (HW) combined with water stress (WS) are critical environmental factors negatively affecting crop development. This study aimed to quantify the individual and combined effects of HW and WS during early reproductive stages on leaf and nodule functioning and their relation with final soybean seed yield (SY). For this purpose, during flowering (R2) and pod formation (R4) soybean (Glycine max L. Merr.) plants were exposed to different temperature (ambient[25ºC] versus HW[40ºC]) and water availability (full capacity versus WS[20% field capacity]). HW, WS and their combined impact on yield depended on the phenological stage at which stress was applied being more affected at R4. For gas exchange, WS severely impaired photosynthetic machinery, especially when combined with HS. Impaired photoassimilate supply at flowering caused flower abortion and a significant reduction in final SY due to interacting stresses and WS. On the other hand, at pod formation (R4), decreased leaf performance caused additive effect on SY by decreasing pod setting and seed size with combined stresses. At the nodule level, WS (alone or in combination with HW) caused nodule impairment, which was reflected by lower leaf N. Such response was linked with a poor malate supply to bacteroids and feed-back inhibition caused by nitrogenous compounds accumulation. In summary, our study noted that soybean sensitivity to interacting heat and water stresses was highly conditioned by the phenological stage at which it occurs with, R4 stage being the critical moment. To our knowledge this is the first soybean work integrating combined stresses at early reproductive stages.
Open Access
Leaf δ15N as a physiological indicator of the responsiveness of N2-fixing alfalfa plants to elevated CO2, temperature and low water availability
(Frontiers Media, 2015) Ariz Arnedo, Idoia; Cruz, Cristina; Neves, Tomé; Irigoyen, Juan J.; García Olaverri, Carmen; Nogués, Salvador; Aparicio Tejo, Pedro María; Aranjuelo Michelena, Iker; Estatistika eta Ikerketa Operatiboa; Natura Ingurunearen Zientziak; Estadística e Investigación Operativa; Ciencias del Medio Natural; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua; Gobierno de Navarra / Nafarroako Gobernua
The natural 15N/14N isotope composition (δ15N) of a tissue is a consequence of its N source and N physiological mechanisms in response to the environment. It could potentially be used as a tracer of N metabolism in plants under changing environmental conditions, where primary N metabolism may be complex, and losses and gains of N fluctuate over time. In order to test the utility of δ15N as an indicator of plant N status in N2-fixing plants grown under various environmental conditions, alfalfa (Medicago sativa L.) plants were subjected to distinct conditions of [CO2] (400 vs. 700 μmol mol−1), temperature (ambient vs. ambient +4°C) and water availability (fully watered vs. water deficiency—WD). As expected, increased [CO2] and temperature stimulated photosynthetic rates and plant growth, whereas these parameters were negatively affected by WD. The determination of δ15N in leaves, stems, roots, and nodules showed that leaves were the most representative organs of the plant response to increased [CO2] and WD. Depletion of heavier N isotopes in plants grown under higher [CO2] and WD conditions reflected decreased transpiration rates, but could also be related to a higher N demand in leaves, as suggested by the decreased leaf N and total soluble protein (TSP) contents detected at 700 μmol mol−1 [CO2] and WD conditions. In summary, leaf δ15N provides relevant information integrating parameters which condition plant responsiveness (e.g., photosynthesis, TSP, N demand, and water transpiration) to environmental conditions.
Open Access
Effect of water stress during grain filling on yield, quality and physiological traits of Illpa and Rainbow quinoa (Chenopodium quinoa willd.) cultivars
(MDPI, 2019) Gámez Guzmán, Angie Lorena; Soba Hidalgo, David; Zamarreño, Ángel M.; García Mina, José M.; Aranjuelo Michelena, Iker; Morales Iribas, Fermín; Ciencias; Zientziak; Gobierno de Navarra / Nafarroako Gobernua
The total area under quinoa (Chenopodium quinoa Willd.) cultivation and the consumption of its grain have increased in recent years because of its nutritional properties and ability to grow under adverse conditions, such as drought. Climate change scenarios predict extended periods of drought and this has emphasized the need for new crops that are tolerant to these conditions. The main goal of this work was to evaluate crop yield and quality parameters and to characterize the physiology of two varieties of quinoa grown under water deficit in greenhouse conditions. Two varieties of quinoa from the Chilean coast (Rainbow) and altiplano (Illpa) were used, grown under full irrigation or two different levels of water deficit applied during the grain filling period. There were no marked differences in yield and quality parameters between treatments, but the root biomass was higher in plants grown under severe water deficit conditions compared to control. Photosynthesis, transpiration and stomatal conductance decreased with increased water stress in both cultivars, but the coastal variety showed higher water use efficiency and less discrimination of13 C under water deficit. This response was associated with greater root development and a better stomatal opening adjustment, especially in the case of Rainbow. The capacity of Rainbow to increase its osmoregulant content (compounds such as proline, glutamine, glutamate, K and Na) could enable a potential osmotic adjustment in this variety. Moreover, the lower stomatal opening and transpiration rates were also associated with higher leaf ABA concentration values detected in Rainbow. We found negative logarithmic relationships between stomatal conductance and leaf ABA concentration in both varieties, with significant R2 values of 0.50 and 0.22 in Rainbow and Illpa, respectively. These moderate-to-medium values suggest that, in addition to ABA signaling, other causes for stomatal closure in quinoa under drought such as hydraulic regulation may play a role. In conclusion, this work showed that two quinoa cultivars use different strategies in the face of water deficit stress, and these prevent decreases in grain yield and quality under drought conditions.
Open Access
Photosynthetic metabolism under stressful growth conditions as a bases for crop breeding and yield improvement
(MDPI, 2020) Morales Iribas, Fermín; Ancín Rípodas, María; Fakhet, Dorra; González Torralba, Jon; Gámez Guzmán, Angie Lorena; Seminario Huárriz, Amaia; Soba Hidalgo, David; Ben Mariem, Sinda; Garriga, Miguel; Aranjuelo Michelena, Iker; Agronomia, Bioteknologia eta Elikadura; Institute for Multidisciplinary Research in Applied Biology - IMAB; Agronomía, Biotecnología y Alimentación
Increased periods of water shortage and higher temperatures, together with a reduction in nutrient availability, have been proposed as major factors that negatively impact plant development. Photosynthetic CO2 assimilation is the basis of crop production for animal and human food, and for this reason, it has been selected as a primary target for crop phenotyping/breeding studies. Within this context, knowledge of the mechanisms involved in the response and acclimation of photosynthetic CO2 assimilation to multiple changing environmental conditions (including nutrients, water availability, and rising temperature) is a matter of great concern for the understanding of plant behavior under stress conditions, and for the development of new strategies and tools for enhancing plant growth in the future. The current review aims to analyze, from a multi-perspective approach (ranging across breeding, gas exchange, genomics, etc.) the impact of changing environmental conditions on the performance of the photosynthetic apparatus and, consequently, plant growth.
Open Access
Estimating peanut and soybean photosynthetic traits using leaf spectral reflectance and advance regression models
(Springer, 2022) Buchaillot, María Luisa; Soba Hidalgo, David; Shu, Tianchu; Liu, Juan; Aranjuelo Michelena, Iker; Araus, José Luis; Runion, G. Brett; Prior, Stephen A.; Kefauver, Shawn C.; Sanz Sáez, Álvaro; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
One proposed key strategy for increasing potential crop stability and yield centers on exploitation of genotypic variability in photosynthetic capacity through precise high-throughput phenotyping techniques. Photosynthetic parameters, such as the maximum rate of Rubisco catalyzed carboxylation (Vc,max) and maximum electron transport rate supporting RuBP regeneration (Jmax), have been identified as key targets for improvement. The primary techniques for measuring these physiological parameters are very time-consuming. However, these parameters could be estimated using rapid and non-destructive leaf spectroscopy techniques. This study compared four different advanced regression models (PLS, BR, ARDR, and LASSO) to estimate Vc,max and Jmax based on leaf reflectance spectra measured with an ASD FieldSpec4. Two leguminous species were tested under different controlled environmental conditions: (1) peanut under different water regimes at normal atmospheric conditions and (2) soybean under high [CO2] and high night temperature. Model sensitivities were assessed for each crop and treatment separately and in combination to identify strengths and weaknesses of each modeling approach. Regardless of regression model, robust predictions were achieved for Vc,max (R2 = 0.70) and Jmax (R2 = 0.50). Field spectroscopy shows promising results for estimating spatial and temporal variations in photosynthetic capacity based on leaf and canopy spectral properties.
Open Access
Elevated CO2 improved the growth of a double nitrate reductase defective mutant of Arabidopsis thaliana: the importance of maintaining a high energy status
(Elsevier, 2017) Jáuregui Mosquera, Iván; Aparicio Tejo, Pedro María; Baroja Fernández, Edurne; Ávila, Concepción; Aranjuelo Michelena, Iker; Natura Ingurunearen Zientziak; Ciencias del Medio Natural; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Impairments in leaf nitrogen (N) assimilation in C3 plants have been identified as processes conditioning photosynthesis under elevated [CO2], especially when N is supplied as nitrate. Leaf N status is usually improved under ammonium nutrition and elevated [CO2]. However, ammonium fertilization is usually accompanied by the appearance of oxidative stress symptoms, which constrains plant development. To understand how the limitations of direct fertilization with ammonium (growth reduction attributed to ammonium toxicity) can be overcome, the effects of elevated [CO2] (800 ppm) exposure were studied in the Arabidopsis thaliana double nitrate reductase defective mutant, nia1-1/chl3-5 (which preferentially assimilates ammonium as its nitrogen source). Analysis of the physiology, metabolites and gene expression was carried out in roots and shoot organs. Our study clearly showed that elevated [CO2] improved the inhibited phenotype of the nitrate reductase double mutant. Both the photosynthetic rates and the leaf N content of the NR mutant under elevated CO2 were similar to wild type plants. The growth of the nitrate reductase mutant was linked to its ability to overcome ammonium-associated photoinhibition processes at 800 ppm [CO2]. More specifically: (i) the capacity of NR mutants to equilibrate energy availability, as reflected by the electron transport equilibrium reached (photosynthesis, photorespiration and respiration), (ii) as well as by the upregulation of genes involved in stress tolerance were identified as the processes involved in the improved performance of NR mutants.
Open Access
Overexpression of thioredoxin m in tobacco chloroplasts inhibits the protein kinase STN7 and alters photosynthetic performance
(Oxford University Press, 2019) Ancín Rípodas, María; Fernández San Millán, Alicia; Larraya Reta, Luis María; Morales Iribas, Fermín; Veramendi Charola, Jon; Aranjuelo Michelena, Iker; Farrán Blanch, Inmaculada; IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
The activity of the protein kinase STN7, involved in phosphorylation of the light-harvesting complex II (LHCII) proteins, has been reported as being co-operatively regulated by the redox state of the plastoquinone pool and the ferredoxin–thioredoxin (Trx) system. The present study aims to investigate the role of plastid Trxs in STN7 regulation and their impact on photosynthesis. For this purpose, tobacco plants overexpressing Trx f or m from the plastid genome were characterized, demonstrating that only Trx m overexpression was associated with a complete loss of LHCII phosphorylation that did not correlate with decreased STN7 levels. The absence of phosphorylation in Trx m-overexpressing plants impeded migration of LHCII from PSII to PSI, with the concomitant loss of PSI–LHCII complex formation. Consequently, the thylakoid ultrastructure was altered, showing reduced grana stacking. Moreover, the electron transport rate was negatively affected, showing an impact on energy-demanding processes such as the Rubisco maximum carboxylation capacity and ribulose 1,5-bisphosphate regeneration rate values, which caused a strong depletion in net photosynthetic rates. Finally, tobacco plants overexpressing a Trx m mutant lacking the reactive redox site showed equivalent physiological performance to the wild type, indicating that the overexpressed Trx m deactivates STN7 in a redox-dependent way.
Open Access
Elevated CO2 has concurrent effects on leaf and grain metabolism but minimal effects on yield in wheat
(Oxford University Press, 2020) Tcherkez, Guillaume; Ben Mariem, Sinda; Larraya Reta, Luis María; García Mina, José M.; Zamarreño, Ángel M.; Paradela, Alberto; Cui, Jing; Badeck, Franz-Werner; Meza, Diego; Rizza, Fulvia; Bunce, James; Han, Xue; Tausz-Posch, Sabine; Cattivelli, Luigi; Fangmeier, Andreas; Aranjuelo Michelena, Iker; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Gobierno de Navarra / Nafarroako Gobernua, PI040 TRIGOCLIM
While the general effect of CO2 enrichment on photosynthesis, stomatal conductance, N content, and yield has been documented, there is still some uncertainty as to whether there are interactive effects between CO2 enrichment and other factors, such as temperature, geographical location, water availability, and cultivar. In addition, the metabolic coordination between leaves and grains, which is crucial for crop responsiveness to elevated CO2, has never been examined closely. Here, we address these two aspects by multi-level analyses of data from several free-air CO2 enrichment experiments conducted in five different countries. There was little effect of elevated CO2 on yield (except in the USA), likely due to photosynthetic capacity acclimation, as reflected by protein profiles. In addition, there was a significant decrease in leaf amino acids (threonine) and macroelements (e.g. K) at elevated CO2, while other elements, such as Mg or S, increased. Despite the non-significant effect of CO2 enrichment on yield, grains appeared to be significantly depleted in N (as expected), but also in threonine, the S-containing amino acid methionine, and Mg. Overall, our results suggest a strong detrimental effect of CO2 enrichment on nutrient availability and remobilization from leaves to grains.