Osés Ruiz, Miriam
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Osés Ruiz
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Miriam
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Agronomía, Biotecnología y Alimentación
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IMAB. Research Institute for Multidisciplinary Applied Biology
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Publication Open Access The phosphorylation landscape of infection-related development by the rice blast fungus(Elsevier, 2024-04-09) Cruz-Mireles, Neftaly; Osés Ruiz, Miriam; Derbyshire, Paul; Jégousse, Clara; Ryder, Lauren S.; Bautista, Mark Jave A.; Eseola, Alice Bisola; Sklenar, Jan; Tang, Bozeng; Yan, Xia; Ma, Weibin; Findlay, Kim C.; Were, Vincent M.; MacLean, Dan; Talbot, Nicholas J.; Menke, Frank L.H.; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta Elikadura; Institute for Multidisciplinary Research in Applied Biology - IMABMany of the world's most devastating crop diseases are caused by fungal pathogens that elaborate specialized infection structures to invade plant tissue. Here, we present a quantitative mass -spectrometry -based phosphoproteomic analysis of infection -related development by the rice blast fungus Magnaporthe oryzae , which threatens global food security. We mapped 8,005 phosphosites on 2,062 fungal proteins following germination on a hydrophobic surface, revealing major re -wiring of phosphorylation-based signaling cascades during appressorium development. Comparing phosphosite conservation across 41 fungal species reveals phosphorylation signatures specifically associated with biotrophic and hemibiotrophic fungal infection. We then used parallel reaction monitoring (PRM) to identify phosphoproteins regulated by the fungal Pmk1 MAPK that controls plant infection by M. oryzae . We define 32 substrates of Pmk1 and show that Pmk1dependent phosphorylation of regulator Vts1 is required for rice blast disease. Defining the phosphorylation landscape of infection therefore identifies potential therapeutic interventions for the control of plant diseases.Publication Open Access The transcriptional landscape of plant infection by the rice blast fungus Magnaporthe oryzae reveals distinct families of temporally co-regulated and structurally conserved effectors(Oxford University Press, 2023) Yan, Xia; Tang, Bozeng; Ryder, Lauren S.; MacLean, Dan; Were, Vincent M.; Eseola, Alice Bisola; Cruz-Mireles, Neftaly; Ma, Weibin; Foster, Andrew J.; Osés Ruiz, Miriam; Talbot, Nicholas J.; Agronomía, Biotecnología y Alimentación; Agronomia, Bioteknologia eta ElikaduraThe rice blast fungus Magnaporthe oryzae causes a devastating disease that threatens global rice (Oryza sativa) production. Despite intense study, the biology of plant tissue invasion during blast disease remains poorly understood. Here we report a high-resolution transcriptional profiling study of the entire plant-associated development of the blast fungus. Our analysis revealed major temporal changes in fungal gene expression during plant infection. Pathogen gene expression could be classified into 10 modules of temporally co-expressed genes, providing evidence for the induction of pronounced shifts in primary and secondary metabolism, cell signaling, and transcriptional regulation. A set of 863 genes encoding secreted proteins are differentially expressed at specific stages of infection, and 546 genes named MEP (Magnaporthe effector protein) genes were predicted to encode effectors. Computational prediction of structurally related MEPs, including the MAX effector family, revealed their temporal co-regulation in the same co-expression modules. We characterized 32 MEP genes and demonstrate that Mep effectors are predominantly targeted to the cytoplasm of rice cells via the biotrophic interfacial complex and use a common unconventional secretory pathway. Taken together, our study reveals major changes in gene expression associated with blast disease and identifies a diverse repertoire of effectors critical for successful infection.