The phosphorylation landscape of infection-related development by the rice blast fungus

Date

2024-04-09

Authors

Cruz-Mireles, Neftaly
Derbyshire, Paul
Jégousse, Clara
Ryder, Lauren S.
Bautista, Mark Jave A.
Eseola, Alice Bisola
Sklenar, Jan
Tang, Bozeng
Yan, Xia

Director

Publisher

Elsevier
Acceso abierto / Sarbide irekia
Artículo / Artikulua
Versión publicada / Argitaratu den bertsioa

Project identifier

  • European Commission/Horizon 2020 Framework Programme/101076478/
  • AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-120188RJ-I00/ES/ recolecta
Impacto
Google Scholar
No disponible en Scopus

Abstract

Many of the world's most devastating crop diseases are caused by fungal pathogens that elaborate specialized infection structures to invade plant tissue. Here, we present a quantitative mass -spectrometry -based phosphoproteomic analysis of infection -related development by the rice blast fungus Magnaporthe oryzae , which threatens global food security. We mapped 8,005 phosphosites on 2,062 fungal proteins following germination on a hydrophobic surface, revealing major re -wiring of phosphorylation-based signaling cascades during appressorium development. Comparing phosphosite conservation across 41 fungal species reveals phosphorylation signatures specifically associated with biotrophic and hemibiotrophic fungal infection. We then used parallel reaction monitoring (PRM) to identify phosphoproteins regulated by the fungal Pmk1 MAPK that controls plant infection by M. oryzae . We define 32 substrates of Pmk1 and show that Pmk1dependent phosphorylation of regulator Vts1 is required for rice blast disease. Defining the phosphorylation landscape of infection therefore identifies potential therapeutic interventions for the control of plant diseases.

Description

Keywords

Appressorium, Pmk1 pathway, Protein phosphorylation, Phosphoproteomics, Magnaporthe oryzaefungus, Signal transduction, MAP kinase, Plant, Pathogenicity

Department

Agronomía, Biotecnología y Alimentación / Agronomia, Bioteknologia eta Elikadura / Institute for Multidisciplinary Research in Applied Biology - IMAB

Faculty/School

Degree

Doctorate program

item.page.cita

Cruz-Mireles, N., Osés-Ruiz, M., Derbyshire, P., Jégousse, C., Ryder, L. S., Bautista, M. J. A., Eseola, A., Sklenar, J., Tang, B. Z., Yan, X., Ma, W. B., Findlay, K. C., Were, V., MacLean, D., Talbot, N. J., Menke, F. L. H. (2024). The phosphorylation landscape of infection-related the rice blast. Cell, 187(10), 2557-2573. https://doi.org/10.1016/j.cell.2024.04.007.

item.page.rights

© 2024 The Author(s). This is an open access article under the CC BY license.

Licencia

Los documentos de Academica-e están protegidos por derechos de autor con todos los derechos reservados, a no ser que se indique lo contrario.