Open Access
Fiber-based label-free D-dimer detection for early diagnosis of venous thromboembolism
(SPIE, 2020) Zubiate Orzanco, Pablo; Urrutia Azcona, Aitor; Ruiz Zamarreño, Carlos; Fernández Irigoyen, Joaquín; Giannetti, Ambra; Baldini, Francesco; Díaz Lucas, Silvia; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Santamaría Martínez, Enrique; Del Villar, Ignacio; Chiavaioli, Francesco; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Institute of Smart Cities - ISC; Ingeniería Eléctrica, Electrónica y de Comunicación
D-dimer is a useful diagnostic biomarker for deep vein thrombosis or pulmonary embolism, collectively referred to as venous thromboembolism (VTE). The ability to detect in real-time the amount of D-dimer with a fast and reliable method is a key step to anticipate the appearance of these diseases. The combination of fiber-optic-based platforms for biosensing with the nanotechnologies is opening up the chance for the development of in situ, portable, lightweight, versatile, reliable and high-performance optical sensing devices towards lab-on-fiber technology. The generation of lossy mode resonances (LMRs) by means of the deposition of nm-thick absorbing metal-oxide films on special geometric-modified fibers allows measuring precisely and accurately surface refractive index changes, which are due to the binding interaction between a biological recognition element and the analyte under investigation. This approach enhances the light-matter interaction in a strong way, thus turning out to be more sensitive compared to other optical technology platforms, such as fiber gratings or surface plasmon resonance. Here, the results of a highly specific and sensitive biosensor for the detection of D-dimer based on LMR in fiber-optics are presented by monitoring in real-time the shift of the LMR related to the biomolecule interactions thanks to a conventional wavelength-interrogation system and an ad-hoc developed microfluidics. A detection limit of 100 ng/mL, a value 5-fold below the clinical cutoff value, has been attained for D-dimer spiked in human serum. The comparison of the results achieved with proteomics-based methodologies, which allows for the identification of betaand gamma-chains of fibrinogen, demonstrates the ability of our platform to specifically (>90%) recognize D-dimer.
Open Access
Experimental demonstration of lossy mode resonance generation for transverse-magnetic and transverse-electric polarizations
(Optica Publishing Group, 2013) Ruiz Zamarreño, Carlos; Zubiate Orzanco, Pablo; Sagüés García, Mikel; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Ingeniería Eléctrica y Electrónica; Ingeniaritza Elektrikoa eta Elektronikoa
This Letter, presents the fabrication of lossy mode resonance (LMR) devices based on titanium dioxide (TiO2)/ poly(sodium 4-styrenesulfonate) (PSS) coatings deposited on side-polished D-shaped optical fibers. TiO2 thin films have been obtained by means of the layer-by-layer (LbL) self-assembly technique. LbL enables us to produce smooth and homogeneous coatings on the polished side of the fiber. This permits us to couple light from the waveguide to the TiO2-coating/external medium region at specific wavelength ranges. The generation of LMRs depends on the coating thickness, so that thicker coatings can produce more resonances. LMRs are sensitive to the external medium refractive index, which allows its utilization as refractometers. The characteristic D-shaped architecture of the devices employed in this Letter enables us to distinguish TE and TM polarizations, which had not been possible before with regular optical fibers due to their cylindrical symmetry. The results presented here show for the first time the experimental demonstration of the generation of LMRs produced by both TM and TE polarizations. More specifically, for these TiO2/PSS thin films, the TM and TM modes of the LMRs show a wavelength shift of 226 nm for the first-order LMR and 56 nm for the second-order LMR.
Open Access
D-shape optical fiber pH sensor based on lossy mode resonances (LMRs)
(IEEE, 2016-01-07) Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Del Villar, Ignacio; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza
The fabrication and characterization of an optical fiber pH sensor based on Lossy Mode Resonances (LMRs) is presented. PAH/PAA polymeric thin-films fabricated onto side-polished D-shaped optical fibers are used as LMR supporting coatings. The thickness of PAH/PAA coatings can be modified as a function of the external medium pH. As a consequence of this variation, the effective refractive index of the structure will change, producing a shift of the LMR. The fabricated sensor has been used to measure pH from 4.0 to 5.0. This pH sensor showed a sensitivity of 101.3 nm per pH unit, which means a resolution of ~6×10-4 pH units by using a conventional communications Optical Spectrum Analyzer (OSA), which is an improvement over commercial pH sensors.
Open Access
D-shape optical fiber refractometer based on TM and TE lossy mode resonances
(SPIE, 2014) Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Del Villar, Ignacio; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza
The fabrication and characterization of an optical fiber refractometer based on Lossy Mode Resonances (LMR) is presented. TiO2/ poly (sodium 4-styrenesulfonate) coatings deposited on side-polished D-shaped optical fibers are used as LMR supporting coatings. LMRs are sensitive to the external medium refractive index and D-shaped optical fibers enable the observation of TE and TM LMR polarizations. These refractometers based on TE and TM LMR showed an average sensitivity of 2737 nm/RIU and 2893 nm/RIU respectively for a surrounding medium refractive index (SMRI) range from 1.35 to 1.41.
Open Access
Giant sensitivity of optical fiber sensors by means of lossy moderesonance
(Elsevier, 2016) Arregui San Martín, Francisco Javier; Del Villar, Ignacio; Ruiz Zamarreño, Carlos; Zubiate Orzanco, Pablo; Matías Maestro, Ignacio; Ingeniaritza Elektrikoa eta Elektronikoa; Institute of Smart Cities - ISC; Ingeniería Eléctrica y Electrónica
Here we show an optical refractometer with a giant sensitivity of 304,360 nm per refractive index unit(nm/RIU). This sensitivity corresponds to a resolution of 3.28 × 10−9RIU if a standard optical spectrumanalyzer with a resolution of 1 pm is used. This record sensitivity is obtained by means of a lossy moderesonance (LMR) optical fiber sensor in a surrounding media with refractive index around 1.45. Thisachievement implies that the utilization of the LMR phenomenon opens the door to devices and systemsthat can beat, in terms of sensitivity, those used currently in real-time biomolecular analysis such assurface plasmon resonance (SPR) devices.
Open Access
A comprehensive review of optical fiber refractometers: toward a standard comparative criterion
(Wiley, 2019) Urrutia Azcona, Aitor; Del Villar, Ignacio; Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Ingeniería Eléctrica, Electrónica y de Comunicación; Ingeniaritza Elektrikoa, Elektronikoa eta Telekomunikazio Ingeniaritza; Institute of Smart Cities - ISC
Thanks to the peculiarities of optical fiber and its ability to be combined with nanotechnology, precise and accurate measurements of the changes in optical properties (i.e., refractive index) of the medium surrounding the fiber are becoming possible with a high degree of performance. Thus, optical fiber sensors (OFSs) are increasingly finding applications in biochemistry and biomedicine. Here, all types of optical fiber refractometers are covered, and they are classified into three main groups: interferometers, grating-based structures, and resonance-based structures (the resonance is induced by coating the optical fiber sensor with a thin film). The performance of these different structures is compared by means of the most common parameters: sensitivity, full width at half minimum or maximum, figure of merit, and quality factor. The aim here is to provide a reliable and easy-to-use tool to compare the performance of the most recent developments on fiber optic refractometers.
Open Access
Optical sensors based on lossy-mode resonances
(Elsevier Science, 2017) Matías Maestro, Ignacio; Ascorbe Muruzabal, Joaquín; Acha Morrás, Nerea de; López Torres, Diego; Zubiate Orzanco, Pablo; Sánchez Zábal, Pedro; Urrutia Azcona, Aitor; Socorro Leránoz, Abián Bentor; Rivero Fuente, Pedro J.; Hernáez Sáenz de Zaitigui, Miguel; Elosúa Aguado, César; Goicoechea Fernández, Javier; Bariáin Aisa, Cándido; Corres Sanz, Jesús María; Ruiz Zamarreño, Carlos; Arregui San Martín, Francisco Javier; Del Villar, Ignacio; Ingeniería Eléctrica y Electrónica; Ingeniaritza Elektrikoa eta Elektronikoa; Institute of Smart Cities - ISC
Open Access
Lossy mode resonances biosensor for the detection of C-reactive protein
(Optica Publishing Group, 2016) Zubiate Orzanco, Pablo; Ruiz Zamarreño, Carlos; Sánchez Zábal, Pedro; Matías Maestro, Ignacio; Arregui San Martín, Francisco Javier; Ingeniería Eléctrica, Electrónica y de Comunicación; Institute of Smart Cities - ISC; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
The fabrication and characterization of optical fiber biosensor based on Lossy Mode Resonances (LMR) to detect C-reactive protein (CRP) are presented. Indium tin oxide (ITO) coatings deposited on side-polished D-shaped optical fibers are used as LMR supporting coatings. The aptamer was immobilized on the ITO film using the Layer-by-Layer (LbL) nano-assembly process. The optical fiber sensor presented shows a high selectivity and low limit detection.
Open Access
Rheumatoid arthritis miRNA biomarker detection by means of LMR based fiber-optic biosensor
(IEEE, 2020) Imas González, José Javier; Ruiz Zamarreño, Carlos; Zubiate Orzanco, Pablo; Campión, J.; Sánchez-Martín, L.; Matías Maestro, Ignacio; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Institute of Smart Cities - ISC; Ingeniería Eléctrica, Electrónica y de Comunicación; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, PJUPNA26
Development of miRNA optical biosensors for disease diagnosis and monitoring has acquired relevance in recent years, due to the clinical importance of miRNA and the inherent advantages of optical sensors. Here, we present the utilization of a fiber optic sensor based on Lossy Mode Resonance (LMR) for the detection of miRNA hsa-miR-223, a promising biomarker for the diagnosis of rheumatoid arthritis (RA).
Open Access
Route towards a label-free optical waveguide sensing platform based on lossy mode resonances
(IFSA Publishing, 2019) Ruiz Zamarreño, Carlos; Zubiate Orzanco, Pablo; Ozcariz Celaya, Aritz; Elosúa Aguado, César; Socorro Leránoz, Abián Bentor; Urrutia Azcona, Aitor; López Torres, Diego; Acha Morrás, Nerea de; Ascorbe Muruzabal, Joaquín; Vitoria Pascual, Ignacio; Imas González, José Javier; Corres Sanz, Jesús María; Díaz Lucas, Silvia; Hernáez Sáenz de Zaitigui, Miguel; Goicoechea Fernández, Javier; Arregui San Martín, Francisco Javier; Matías Maestro, Ignacio; Del Villar, Ignacio; Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren; Institute of Smart Cities - ISC; Ingeniería Eléctrica, Electrónica y de Comunicación; Gobierno de Navarra / Nafarroako Gobernua,0011-1365-2017- 000117; Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, PJUPNA26
According to recent market studies of the North American company Allied Market Research, the field of photonic sensors is an emerging strategic field for the following years and it is expected to garner $18 billion by 2021. The integration of micro and nanofabrication technologies in the field of sensors has allowed the development of new technological concepts such as lab-on-a-chip which have achieved extraordinary advances in terms of detection and applicability, for example in the field of biosensors. This continuous development has allowed that equipment consisting of many complex devices that occupied a whole room a few years ago, at present it is possible to handle them in the palm of the hand; that formerly long duration processes are carried out in a matter of milliseconds and that a technology previously dedicated solely to military or scientific uses is available to the vast majority of consumers. The adequate combination of micro and nanostructured coatings with optical fiber sensors has permitted us to develop novel sensing technologies, such as the first experimental demonstration of lossy mode resonances (LMRs) for sensing applications, with more than one hundred citations and related publications in high rank journals and top conferences. In fact, fiber optic LMR-based devices have been proven as devices with one of the highest sensitivity for refractometric applications. Refractive index sensitivity is an indirect and simple indicator of how sensitive the device is to chemical and biological species, topic where this proposal is focused. Consequently, the utilization of these devices for chemical and biosensing applications is a clear opportunity that could open novel and interesting research lines and applications as well as simplify current analytical methodologies. As a result, on the basis of our previous experience with LMR based sensors to attain very high sensitivities, the objective of this paper is presenting the route for the development of label-free optical waveguide sensing platform based on LMRs that enable to explore the limits of this technology for bio-chemosensing applications.