Publication: Brucellosis vaccines: assessment of brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and export
dc.contributor.author | González, David | |
dc.contributor.author | Grilló Dolset, María Jesús | |
dc.contributor.author | Miguel, María Jesús de | |
dc.contributor.author | Ali, Tara | |
dc.contributor.author | Arce Gorvel, Vilma | |
dc.contributor.author | Delrue, Rose May | |
dc.contributor.author | Conde Álvarez, Raquel | |
dc.contributor.author | Muñoz Álvaro, Pilar María | |
dc.contributor.author | López Goñi, Ignacio | |
dc.contributor.author | Iriarte, Maite | |
dc.contributor.author | Marín, Clara M. | |
dc.contributor.author | Weintraub, Andrej | |
dc.contributor.author | Widmalm, Goeran | |
dc.contributor.author | Zygmunt, Michel | |
dc.contributor.author | Letesson, Jean-Jacques | |
dc.contributor.author | Gorvel, Jean-Pierre | |
dc.contributor.author | Blasco Canet, José María | |
dc.contributor.author | Moriyón Uría, Ignacio | |
dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es_ES |
dc.date.accessioned | 2014-06-11T09:18:09Z | |
dc.date.available | 2014-06-11T09:18:09Z | |
dc.date.issued | 2008 | |
dc.description.abstract | Background: The brucellae are facultative intracellular bacteria that cause brucellosis, one of the major neglected zoonoses. In endemic areas, vaccination is the only effective way to control this disease. Brucella melitensis Rev 1 is a vaccine effective against the brucellosis of sheep and goat caused by B. melitensis, the commonest source of human infection. However, Rev 1 carries a smooth lipopolysaccharide with an O-polysaccharide that elicits antibodies interfering in serodiagnosis, a major problem in eradication campaigns. Because of this, rough Brucella mutants lacking the O-polysaccharide have been proposed as vaccines. Methodology/Principal Findings: To examine the possibilities of rough vaccines, we screened B. melitensis for lipopolysaccharide genes and obtained mutants representing all main rough phenotypes with regard to core oligosaccharide and O-polysaccharide synthesis and export. Using the mouse model, mutants were classified into four attenuation patterns according to their multiplication and persistence in spleens at different doses. In macrophages, mutants belonging to three of these attenuation patterns reached the Brucella characteristic intracellular niche and multiplied intracellularly, suggesting that they could be suitable vaccine candidates. Virulence patterns, intracellular behavior and lipopolysaccharide defects roughly correlated with the degree of protection afforded by the mutants upon intraperitoneal vaccination of mice. However, when vaccination was applied by the subcutaneous route, only two mutants matched the protection obtained with Rev 1 albeit at doses one thousand fold higher than this reference vaccine. These mutants, which were blocked in O-polysaccharide export and accumulated internal O-polysaccharides, stimulated weak anti-smooth lipopolysaccharide antibodies. Conclusions/Significance: The results demonstrate that no rough mutant is equal to Rev 1 in laboratory models and question the notion that rough vaccines are suitable for the control of brucellosis in endemic areas. | en |
dc.description.sponsorship | This work was funded by the European Commission (Research Contract QLK2-CT-2002-00918) and the Ministerio de Ciencia y Tecnología of Spain (Proyecto AGL2004-01162/GAN). | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.doi | 10.1371/journal.pone.0002760 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | 738 | |
dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/10815 | |
dc.language.iso | eng | en |
dc.publisher | Public Library of Science | en |
dc.relation.ispartof | Plos One, 2008, 3(7): e2760 | en |
dc.relation.publisherversion | https://dx.doi.org/10.1371/journal.pone.0002760 | |
dc.rights | © 2008 González et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/ | |
dc.subject | Brucellosis vaccines | en |
dc.subject | Brucella melitensis | en |
dc.subject | O-polysaccharide | en |
dc.subject | Rough vaccines | en |
dc.subject | Mutants | en |
dc.subject | Rev 1 | en |
dc.title | Brucellosis vaccines: assessment of brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and export | en |
dc.type | info:eu-repo/semantics/article | |
dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 2eb9ac0b-533e-4c1d-ad93-f81b1fadee74 | |
relation.isAuthorOfPublication.latestForDiscovery | 2eb9ac0b-533e-4c1d-ad93-f81b1fadee74 |