A cAMP/CRP-controlled mechanism for the incorporation of extracellular ADP-glucose in Escherichia coli involving NupC and NupG nucleoside transporters

Almagro Zabalza, Goizeder; Viale, Alejandro M.; Montero Macarro, Manuel; Muñoz Pérez, Francisco José; Baroja Fernández, Edurne; Mori, Hirotada; Pozueta Romero, Javier

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A cAMP/CRP-controlled mechanism for the incorporation of extracellular ADP-glucose in Escherichia coli involving NupC and NupG nucleoside transporters

dc.contributor.author	Almagro Zabalza, Goizeder
dc.contributor.author	Viale, Alejandro M.
dc.contributor.author	Montero Macarro, Manuel
dc.contributor.author	Muñoz Pérez, Francisco José
dc.contributor.author	Baroja Fernández, Edurne
dc.contributor.author	Mori, Hirotada	es_ES
dc.contributor.author	Pozueta Romero, Javier
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2019-07-01T09:27:37Z
dc.date.available	2019-07-01T09:27:37Z
dc.date.issued	2018
dc.description.abstract	ADP-glucose is the precursor of glycogen biosynthesis in bacteria, and a compound abundant in the starchy plant organs ingested by many mammals. Here we show that the enteric species Escherichia coli is capable of scavenging exogenous ADP-glucose for use as a glycosyl donor in glycogen biosynthesis and feed the adenine nucleotide pool. To unravel the molecular mechanisms involved in this process, we screened the E. coli single-gene deletion mutants of the Keio collection for glycogen content in ADP-glucose-containing culture medium. In comparison to wild-type (WT) cells, individual ∆nupC and ∆nupG mutants lacking the cAMP/CRP responsive inner-membrane nucleoside transporters NupC and NupG displayed reduced glycogen contents and slow ADP-glucose incorporation. In concordance, ∆cya and ∆crp mutants accumulated low levels of glycogen and slowly incorporated ADP-glucose. Two-thirds of the glycogen-excess mutants identified during screening lacked functions that underlie envelope biogenesis and integrity, including the RpoE specific RseA anti-sigma factor. These mutants exhibited higher ADP-glucose uptake than WT cells. The incorporation of either ∆crp, ∆nupG or ∆nupC null alleles sharply reduced the ADP-glucose incorporation and glycogen content initially witnessed in ∆rseA cells. Overall, the data showed that E. coli incorporates extracellular ADP-glucose through a cAMP/CRP-regulated process involving the NupC and NupG nucleoside transporters that is facilitated under envelope stress conditions.	en
dc.description.sponsorship	This work was partially supported by the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) (grant numbers BIO2013-49125-C2-1-P and BIO2016-78747-P). A.M.V. is a Career Researcher of the Consejo Nacional de Investigaciones Cientificas y Técnicas de Argentina (CONICET) and Professor of Micribiology at the National University of Rosario (U.N.R., Argentina). A.M.V. expresses his gratitude to the Ministerio de Educación y Cultura, the Consejo Superior de Investigaciones Científicas, and the Public University of Navarra for financial support.	en
dc.format.extent	15 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1038/s41598-018-33647-w
dc.identifier.issn	2045-2322
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/33506
dc.language.iso	eng	en
dc.publisher	Nature Research	en
dc.relation.ispartof	Scientific Reports, (2018) 8:15509	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//BIO2013-49125-C2-2-P/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/ES/1PE/BIO2016-78747-P	en
dc.relation.publisherversion	https://doi.org/10.1038/s41598-018-33647-w
dc.rights	© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	ADP-glucose	en
dc.subject	Escherichia coli	en
dc.subject	NupC	en
dc.subject	NupG	en
dc.subject	cAMP/CRP	en
dc.title	A cAMP/CRP-controlled mechanism for the incorporation of extracellular ADP-glucose in Escherichia coli involving NupC and NupG nucleoside transporters	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
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