Publication:
Sucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch production

Simple item page
dc.contributor.authorBaroja Fernández, Edurne
dc.contributor.authorMuñoz Pérez, Francisco José
dc.contributor.authorLi, Jun
dc.contributor.authorBahaji, Abdellatif
dc.contributor.authorAlmagro Zabalza, Goizeder
dc.contributor.authorMontero Macarro, Manuel
dc.contributor.authorEtxeberria, Ed
dc.contributor.authorHidalgo Cruz, Maitees_ES
dc.contributor.authorSesma Pascual, María Teresa
dc.contributor.authorPozueta Romero, Javier
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2018-12-28T09:14:04Z
dc.date.available2018-12-28T09:14:04Z
dc.date.issued2011
dc.description.abstractSucrose synthase (SUS) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate-glucose and fructose. In Arabidopsis, a multigene family encodes six SUS (SUS1-6) isoforms. The involvement of SUS in the synthesis of UDP-glucose and ADP-glucose linked to Arabidopsis cellulose and starch biosynthesis, respectively, has been questioned by Barratt et al. [(2009) Proc Natl Acad Sci USA 106:13124–13129], who showed that (i) SUS activity in wild type (WT) leaves is too low to account for normal rate of starch accumulation in Arabidopsis, and (ii) different organs of the sus1/sus2/sus3/sus4 SUS mutant impaired in SUS activity accumulate WT levels of ADP-glucose, UDP-glucose, cellulose and starch. However, these authors assayed SUS activity under unfavorable pH conditions for the reaction. By using favorable pH conditions for assaying SUS activity, in this work we show that SUS activity in the cleavage direction is sufficient to support normal rate of starch accumulation in WT leaves. We also demonstrate that sus1/sus2/sus3/sus4 leaves display WT SUS5 and SUS6 expression levels, whereas leaves of the sus5/sus6 mutant display WT SUS1–4 expression levels. Furthermore, we show that SUS activity in leaves and stems of the sus1/sus2/sus3/sus4 and sus5/sus6 plants is ~85% of that of WT leaves, which can support normal cellulose and starch biosynthesis. The overall data disprove Barratt et al. (2009) claims, and are consistent with the possible involvement of SUS in cellulose and starch biosynthesis in Arabidopsis.en
dc.description.sponsorshipThis research was partially supported by the Grant BIO2010-18239 from the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain), and by Iden Biotechnology S.L. G.A. acknowledges a fellowship from the Public University of Navarra.en
dc.format.extent6 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1073/pnas.1117099109
dc.identifier.issn0027-8424 (Print)
dc.identifier.issn1091-6490 (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/31885
dc.language.isoengen
dc.publisherNational Academy of Sciencesen
dc.relation.ispartofPNAS, January 3, 2012 109 (1) 321-326en
dc.relation.publisherversionhttps://doi.org/10.1073/pnas.1117099109
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.subjectCarbohydrate metabolismen
dc.subjectSink strengthen
dc.titleSucrose synthase activity in the sus1/sus2/sus3/sus4 Arabidopsis mutant is sufficient to support normal cellulose and starch productionen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dspace.entity.typePublication
relation.isAuthorOfPublication0a533d7e-81fb-4439-ad05-2927e96fa923
relation.isAuthorOfPublicationfe94cbef-f6fb-4757-93d4-7ec184ca08ef
relation.isAuthorOfPublication57646c04-3fff-48b2-9167-633066a5d3f9
relation.isAuthorOfPublication98c9658b-9c03-41bf-9966-776fa23ec157
relation.isAuthorOfPublicationbcd76277-79d9-4cf7-b6bd-f172dabe984b
relation.isAuthorOfPublicationf1ef3ebe-f00b-4da7-90d2-7175950762aa
relation.isAuthorOfPublication78b7c259-c40f-41dd-bdbf-9a39c87cf07f
relation.isAuthorOfPublication4f2b9025-5eba-4174-ba9d-74342c619e78
relation.isAuthorOfPublication80c8bd69-4127-4474-a679-3b10747cae42
relation.isAuthorOfPublication.latestForDiscoveryf1ef3ebe-f00b-4da7-90d2-7175950762aa

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
19_Baroja_SucroseSynthase.pdf
Size:
654.26 KB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
19_MatCompl.zip
Size:
858.12 KB
Format:
ZIP
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download

Collections

Artículos de revista IdAB - IdAB Aldizkari artikuluak
Artículos de revista - Aldizkari artikuluak