The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis
| dc.contributor.author | Pereira, Catia | |
| dc.contributor.author | Montalbán, Itziar A. | |
| dc.contributor.author | Goicoa Mangado, Tomás | |
| dc.contributor.author | Ugarte Martínez, María Dolores | |
| dc.contributor.author | Correia, Sandra | |
| dc.contributor.author | Canhoto, Jorge M. | |
| dc.contributor.author | Moncaleán, Paloma | |
| dc.contributor.department | Estadística e Investigación Operativa | es_ES |
| dc.contributor.department | Estatistika eta Ikerketa Operatiboa | eu |
| dc.date.accessioned | 2018-09-04T06:43:46Z | |
| dc.date.available | 2018-09-04T06:43:46Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain). Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage. Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages. Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability. | en |
| dc.description.sponsorship | MINECO, Spanish Government (project AGL2013-4700-C4-2R). | en |
| dc.format.extent | 4 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.5424/fs/2017263-11436 | |
| dc.identifier.issn | 2171-9845 (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/30428 | |
| dc.language.iso | eng | en |
| dc.publisher | Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) | es_ES |
| dc.relation.ispartof | Forest Systems, 26 (3), eSC05, 4 pages (2017) | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//AGL2013-47000-R/ES/ | |
| dc.relation.publisherversion | https://doi.org/10.5424/fs/2017263-11436 | |
| dc.rights | © 2017 INIA. This is an open access article distributed under the terms of the Creative Commons Attribution (CC-by) Spain 3.0 License. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by/3.0/es/deed.es/ | |
| dc.subject | Embryogenic cell lines | en |
| dc.subject | Pinus halepensis | en |
| dc.subject | Somatic embryos | en |
| dc.subject | Water availability | en |
| dc.title | The effect of changing temperature and agar concentration at proliferation stage in the final success of Aleppo pine somatic embryogenesis | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
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| relation.isAuthorOfPublication.latestForDiscovery | 77ba75f3-a30d-4f01-8cc5-9de6d3a10d8d |