The pattern of shikimate pathway and phenylpropanoids after inhibition by glyphosate or quinate feeding in pea roots

Zabalza Aznárez, Ana; Orcaray Echeverría, Luis; Fernández Escalada, Manuel; Zulet González, Ainhoa; Royuela Hernando, Mercedes

Publication:
The pattern of shikimate pathway and phenylpropanoids after inhibition by glyphosate or quinate feeding in pea roots

dc.contributor.author	Zabalza Aznárez, Ana
dc.contributor.author	Orcaray Echeverría, Luis
dc.contributor.author	Fernández Escalada, Manuel
dc.contributor.author	Zulet González, Ainhoa
dc.contributor.author	Royuela Hernando, Mercedes
dc.contributor.department	Ciencias del Medio Natural	es_ES
dc.contributor.department	Natura Ingurunearen Zientziak	eu
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2017-09-13T07:28:58Z
dc.date.available	2018-09-01T23:00:12Z
dc.date.issued	2017
dc.description.abstract	The shikimate pathway is a metabolic route for the biosynthesis of aromatic amino acids (AAAs) (i.e. phenylalanine, tyrosine, and tryptophan). A key enzyme of shikimate pathway (5-enolpyruvylshikimate-3-phosphate synthase, EPSPS) is the target of the widely used herbicide glyphosate. Quinate is a compound synthesized in plants through a side branch of the shikimate pathway. Glyphosate provokes quinate accumulation and exogenous quinate application to plants shows a potential role of quinate in the toxicity of the herbicide glyphosate. Based on this, we hypothesized that the role of quinate accumulation in the toxicity of the glyphosate would be mediated by a deregulation of the shikimate pathway. In this study the effect of the glyphosate and of the exogenous quinate was evaluated in roots of pea plants by analyzing the time course of a full metabolic map of several metabolites of shikimate and phenylpropanoid pathways. Glyphosate application induced an increase of the 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHPS, first enzyme of the shikimate pathway) protein and accumulation of metabolites upstream of the enzyme EPSPS. No common effects on the metabolites and regulation of shikimate pathway were detected between quinate and glyphosate treatments, supporting that the importance of quinate in the mode of action of glyphosate is not mediated by a common alteration of the regulation of the shikimate pathway. Contrary to glyphosate, the exogenous quinate supplied was probably incorporated into the main trunk from the branch pathway and accumulated in the final products, such as lignin, concomitant with a decrease in the amount of DAHPS protein.	en
dc.description.sponsorship	This work was financially supported by a grant from the Ministerio Español de Economía y Competitividad (AGL-2013-40567R). L.O. received a grant from the Ministerio de Educación. M. F-E and AZ-G received funding from fellowships from Universidad Pública de Navarra.	en
dc.embargo.lift	2018-09-01
dc.embargo.terms	2018-09-01
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1016/j.pestbp.2016.12.005
dc.identifier.issn	0048-3575 (Print)
dc.identifier.issn	1095-9939 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/25560
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Pesticide Biochemistry and Physiology 141 (2017) 96–102	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2013-40567-R/ES/	en
dc.relation.publisherversion	https://doi.org/10.1016/j.pestbp.2016.12.005
dc.relation.publisherversion	https://dx.doi.org/10.1016/j.plantsci.2017.08.003
dc.rights	© 2016 Elsevier Inc. The manuscript version is made available under the CC BY-NC-ND 4.0 license.	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Aromatic amino acids	en
dc.subject	DAHPS	en
dc.subject	EPSPS	en
dc.subject	Phenylpropanoid metabolism	en
dc.subject	Hydroxybenzoic acids	en
dc.subject	Hydroxycinnamic acids	en
dc.title	The pattern of shikimate pathway and phenylpropanoids after inhibition by glyphosate or quinate feeding in pea roots	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dspace.entity.type	Publication
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