Both free indole-3-acetic acid and photosynthetic performance are important players in the response of Medicago truncatula to urea and ammonium nutrition under axenic conditions

Esteban Terradillos, Raquel; Royo Castillejo, Beatriz; Urarte Rodríguez, Estíbaliz; Zamarreño, Ángel M.; García Mina, José M.; Morán Juez, José Fernando

Publication:
Both free indole-3-acetic acid and photosynthetic performance are important players in the response of Medicago truncatula to urea and ammonium nutrition under axenic conditions

dc.contributor.author	Esteban Terradillos, Raquel
dc.contributor.author	Royo Castillejo, Beatriz
dc.contributor.author	Urarte Rodríguez, Estíbaliz
dc.contributor.author	Zamarreño, Ángel M.
dc.contributor.author	García Mina, José M.
dc.contributor.author	Morán Juez, José Fernando
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2018-09-04T06:43:50Z
dc.date.available	2018-09-04T06:43:50Z
dc.date.issued	2016
dc.description.abstract	We aimed to identify the early stress response and plant performance of Medicago truncatula growing in axenic medium with ammonium or urea as the sole source of nitrogen, with respect to nitrate-based nutrition. Biomass measurements, auxin content analyses, root system architecture (RSA) response analyses, and physiological parameters were determined. Both ammonium and ureic nutrition severely affected the RSA, resulting in changes in the main elongation rate, lateral root development, and insert position from the root base. The auxin content decreased in both urea- and ammonium-treated roots; however, only the ammonium-treated plants were affected at the shoot level. The analysis of chlorophyll a fluorescence transients showed that ammonium affected photosystem II, but urea did not impair photosynthetic activity. Superoxide dismutase isoenzymes in the plastids were moderately affected by urea and ammonium in the roots. Overall, our results showed that low N doses from different sources had no remarkable effects on M. truncatula, with the exception of the differential phenotypic root response. High doses of both ammonium and urea caused great changes in plant length, auxin contents and physiological measurements. Interesting correlations were found between the shoot auxin pool and both plant length and the “performance index” parameter, which is obtained from measurements of the kinetics of chlorophyll a fluorescence. Taken together, these data demonstrate that both the indole-3-acetic acid pool and performance index are important components of the response of M. truncatula under ammonium or urea as the sole N source.	en
dc.description.sponsorship	This work was supported by the grants AGL2010-16167 and AGL2014-52396-from the Spanish Ministry of Economy and Competitiviness-Mineco. BR is a holder of a PhD fellowship from the Mineco. RE received a JAE-Doc-2011-046 contract from the Spanish Research Council (CSIC) of the programme "Junta para la Ampliación de Estudios" co-financed by the European Social Fund.	en
dc.format.extent	14 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.3389/fpls.2016.00140
dc.identifier.issn	1664-462X (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30440
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Plant Science, 7:140	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2014-52396-P/ES/	en
dc.relation.publisherversion	https://doi.org/10.3389/fpls.2016.00140
dc.rights	© 2016 Esteban, Royo, Urarte, Zamarreño, Garcia-Mina and Moran. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Ammonium	en
dc.subject	Auxin	en
dc.subject	OJIP curve	en
dc.subject	Nitrate	en
dc.subject	Root system architecture	en
dc.subject	Urea	en
dc.title	Both free indole-3-acetic acid and photosynthetic performance are important players in the response of Medicago truncatula to urea and ammonium nutrition under axenic conditions	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
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