Efficient and scalable gene delivery method with easily generated cationic carbon dots

Algarra González, Manuel; González-Muñoz, Elena

Publication:
Efficient and scalable gene delivery method with easily generated cationic carbon dots

dc.contributor.author	Algarra González, Manuel
dc.contributor.author	González-Muñoz, Elena
dc.contributor.department	Ciencias	es_ES
dc.contributor.department	Zientziak	eu
dc.contributor.department	Institute for Advanced Materials and Mathematics - INAMAT2	en
dc.date.accessioned	2024-05-30T18:34:08Z
dc.date.available	2024-05-30T18:34:08Z
dc.date.issued	2024
dc.date.updated	2024-05-30T18:17:29Z
dc.description.abstract	Gene delivery is a complex process with several challenges when attempting to incorporate genetic material efficiently and safely into target cells. Some of the key challenges include not only efficient cellular uptake and endosomal escape to ensure that the genetic material can exert its effect but also minimizing the toxicity of the delivery system, which is vital for safe gene delivery. Of importance, if gene delivery systems are intended for biomedical applications or clinical use, they must be scalable and easy and affordable to manufacture to meet the demand. Here, we show an efficient gene delivery method using a combination of carbon dots coated by PEI through electrostatic binding to easily generate cationic carbon dots. We show a biofunctional approach to generate optimal cationic carbon dots (CCDs) that can be scaled up to meet specific transfection demands. CCDs improve cell viability and increase transfection efficiency four times over the standard of PEI polyplexes. Generated CCDs enabled the challenging transfection protocol to produce retroviral vectors via cell cotransfection of three different plasmids into packing cells, showing not only high efficiency but also functionality of the gene delivery, tested as the capacity to produce infective retroviral particles.	en
dc.description.sponsorship	From Ministerio de Ciencia e Innovación del Gobierno de España (grant number PID2021-124033OB-I00), from Consejería Economía y Conocimiento Junta de Andalucía-FEDER (grant number UMA18-FEDERJA-107) and from Ministry of Science and Innovation (MCIN/AEI/https://doi.org/10.13039/501100011033) for financial support (grant number PID2021-122613OB-I00).	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Algarra, M., Gonzalez-Muñoz, E. (2024) Efficient and scalable gene delivery method with easily generated cationic carbon dots. Biological Procedures Online, 26, 6, 1-11. https://doi.org/10.1186/s12575-024-00232-7.	en
dc.identifier.doi	10.1186/s12575-024-00232-7
dc.identifier.issn	1480-9222
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/48217
dc.language.iso	eng	en
dc.publisher	BMC	en
dc.relation.ispartof	Biological Procedures Online 26:6, (2024)	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-124033OB-I00/ES/	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-122613OB-I00/ES/	en
dc.relation.publisherversion	https://doi.org/10.1186/s12575-024-00232-7
dc.rights	© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Carbon dots	en
dc.subject	Polyethyleneimine (PEI)	en
dc.subject	Cell transfection	en
dc.subject	Gene delivery	en
dc.subject	Cell transduction	en
dc.title	Efficient and scalable gene delivery method with easily generated cationic carbon dots	en
dc.type	info:eu-repo/semantics/article
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
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