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Depletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plants

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dc.contributor.authorAriz Arnedo, Idoia
dc.contributor.authorCruz, Cristina
dc.contributor.authorMorán Juez, José Fernando
dc.contributor.authorGonzález Moro, María Begoña
dc.contributor.authorGarcía Olaverri, Carmen
dc.contributor.authorGonzález Murua, Carmen
dc.contributor.authorMartins Loucao, María A.
dc.contributor.authorAparicio Tejo, Pedro María
dc.contributor.departmentEstatistika eta Ikerketa Operatiboaeu
dc.contributor.departmentEstadística e Investigación Operativaes_ES
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2014-06-05T11:28:59Z
dc.date.available2014-06-05T11:28:59Z
dc.date.issued2011
dc.description.abstractBackground: In plants, nitrate (NO3-) nutrition gives rise to a natural N isotopic signature (δ15N), which correlates with the δ15N of the N source. However, little is known about the relationship between the δ15N of the N source and the 14N/15N fractionation in plants under ammonium (NH4+) nutrition. When NH4 + is the major N source, the two forms, NH4 + and NH3, are present in the nutrient solution. There is a 1.025 thermodynamic isotope effect between NH3 (g) and NH4 + (aq) which drives to a different δ15N. Nine plant species with different NH4 +-sensitivities were cultured hydroponically with NO3 - or NH4 + as the sole N sources, and plant growth and δ15N were determined. Short-term NH4 +/NH3 uptake experiments at pH 6.0 and 9.0 (which favours NH3 form) were carried out in order to support and substantiate our hypothesis. N source fractionation throughout the whole plant was interpreted on the basis of the relative transport of NH4 + and NH3. Results: Several NO3 --fed plants were consistently enriched in 15N, whereas plants under NH4 + nutrition were depleted of 15N. It was shown that more sensitive plants to NH4 + toxicity were the most depleted in 15N. In parallel, N-deficient pea and spinach plants fed with 15NH4 + showed an increased level of NH3 uptake at alkaline pH that was related to the 15N depletion of the plant. Tolerant to NH4 + pea plants or sensitive spinach plants showed similar trend on 15N depletion while slight differences in the time kinetics were observed during the initial stages. The use of RbNO3 as control discarded that the differences observed arise from pH detrimental effects. Conclusions: This article proposes that the negative values of δ15N in NH4 +-fed plants are originated from NH3 uptake by plants. Moreover, this depletion of the heavier N isotope is proportional to the NH4 +/NH3 toxicity in plants species. Therefore, we hypothesise that the low affinity transport system for NH4 + may have two components: one that transports N in the molecular form and is associated with fractionation and another that transports N in the ionic form and is not associated with fractionation.en
dc.description.sponsorshipThis work was supported by the Spanish MICIIN (grant nos. AGL2006-12792-CO2- 01 and 02 and AGL2009- 13339-CO2-01 and 02 [to P.A.-T. and C.G.M.] and AGL2007-64432/AGR [to J.F.M.]), by the Portuguese FCT (PTDC/BIA- BEC/ 099323/2008) and by the Basque Government IT526-10.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1186/1471-2229-11-83
dc.identifier.issn1471-2229
dc.identifier.other579
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/10764
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.ispartofBMC Plant Biology, 2011, 11: 83en
dc.relation.publisherversionhttps://dx.doi.org/10.1186/1471-2229-11-83
dc.rights© 2011 Ariz et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.subjectLow affinity ammonium transportersen
dc.subjectNitrogen isotopic signatureen
dc.subjectAmmonium/ammoniaen
dc.subjectAmmonium dissociation isotope factoren
dc.subjectAmmonia uptakeen
dc.subjectGDP-Mannose pyrophosphorylaseen
dc.subjectRoot growth Inhibitionen
dc.subjectNitrogenen
dc.subjectIsotopesen
dc.subjectAmmonium transporteren
dc.subjectGlutamine synthetaseen
dc.subjectPlasma membraneen
dc.subjectPhosphoenolpyruvate carboxylaseen
dc.subjectArabidopsis thalianaen
dc.subjectPotassiumen
dc.subjectChannelen
dc.subjectNatural abundanceen
dc.titleDepletion of the heaviest stable N isotope is associated with NH4+/NH3 toxicity in NH4+-fed plantsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dspace.entity.typePublication
relation.isAuthorOfPublication6f7563e2-1f07-4a25-8b38-4ac55bd18224
relation.isAuthorOfPublicationcad13109-2660-4ec4-80c8-e4bcc0685382
relation.isAuthorOfPublication5b37dbbe-41f2-4180-b1d7-9f64876eaa5b
relation.isAuthorOfPublication19699fdc-1098-4a1c-b547-7849e8634c2a
relation.isAuthorOfPublication.latestForDiscovery6f7563e2-1f07-4a25-8b38-4ac55bd18224

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