Multidrug resistant Salmonella enterica isolated from conventional pig farms using antimicrobial agents in preventative medicine programmes

Cameron Veasa, Karla; Fraile, Lorenzo; Nappa, Sebastian; Garrido González, Victoria; Grilló Dolset, María Jesús

Multidrug resistant Salmonella enterica isolated from conventional pig farms using antimicrobial agents in preventative medicine programmes

dc.contributor.author	Cameron Veasa, Karla
dc.contributor.author	Fraile, Lorenzo
dc.contributor.author	Nappa, Sebastian
dc.contributor.author	Garrido González, Victoria
dc.contributor.author	Grilló Dolset, María Jesús
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua, IIQ14064.RI1	es
dc.date.accessioned	2020-11-11T10:24:55Z
dc.date.available	2020-11-11T10:24:55Z
dc.date.issued	2018
dc.description.abstract	A longitudinal study was conducted to investigate the presence of multidrug antimicrobial resistance (multi-AR) in Salmonella enterica in pigs reared under conventional preventative medicine programmes in Spain and the possible association of multi-AR with ceftiofur or tulathromycin treatment during the pre-weaning period. Groups of 7-day-old piglets were treated by intramuscular injection with ceftiofur on four farms (n = 40 piglets per farm) and with tulathromycin on another four farms (n = 40 piglets per farm). A control group of untreated piglets (n = 30 per farm) was present on each farm. Faecal swabs were collected for S. enterica culture prior to treatment, at 2, 7 and 180 days post-treatment, and at slaughter. Minimal inhibitory concentrations of 14 antimicrobial agents, pulsed-field gel electrophoresis and detection of resistance genes representing five families of antimicrobial agents were performed. Plasmids carrying cephalosporin resistant (CR) genes were characterised. Sixty-six S. enterica isolates were recovered from five of eight farms. Forty-seven isolates were multi-AR and four contained blaCTX-M genes harboured in conjugative plasmids of the IncI1 family; three of these isolates were recovered before treatment with ceftiofur. The most frequent AR genes detected were tet(A) (51/66, 77%), sul1 (17/66, 26%); tet(B) (15/66, 23%) and qnrB (10/66, 15%). A direct relation between the use of ceftiofur in these conditions and the occurrence of CR S. enterica was not established. However, multi-AR was common, especially for ampicillin, streptomycin, sulphonamides and tetracycline. These antibiotics are used frequently in veterinary medicine in Spain and, therefore, should be used sparingly to minimise the spread of multi-AR.	en
dc.description.sponsorship	This work was supported by project AGL2011-28836 from the Ministerio de Economía y Competitividad of Spain, the Departamento de Innovación, Empresa y Empleo del Gobierno de Navarra (project reference IIQ14064.RI1) and Fundación Caja Navarra, Spain (project reference VATC 2014-0411 y VATC 2015-70628). Contract of LMG was supported by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) and the European Social Fund. Contract of VG was supported by Fundación Caja Navarra, Spain (project reference VATC 2015-70628). The authors acknowledge the Centres de Recerca de Catalunya programme for financial support.	en
dc.format.extent	7 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1016/j.tvjl.2018.02.002
dc.identifier.issn	1090-0233
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/38588
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	The Veterinary Journal, 2018, 234, 36-42	en
dc.relation.projectID	info:eu-repo/grantAgreement/MICINN//AGL2011-28836/ES/
dc.relation.publisherversion	https://doi.org/10.1016/j.tvjl.2018.02.002
dc.rights	© 2018 The Authors. This is an open access article under the CC BY-NC-ND license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Ceftiofur	en
dc.subject	b-Lactams	en
dc.subject	Multidrug resistance	en
dc.subject	Pig	en
dc.subject	Salmonella enterica	en
dc.title	Multidrug resistant Salmonella enterica isolated from conventional pig farms using antimicrobial agents in preventative medicine programmes	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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