Circular RNA expression profile in blood according to ischemic stroke etiology

Ostolaza, Aiora; Blanco Luquin, Idoia; Urdánoz Casado, Amaya; Rubio, Idoya; Labarga Gutiérrez, Alberto

Circular RNA expression profile in blood according to ischemic stroke etiology

dc.contributor.author	Ostolaza, Aiora
dc.contributor.author	Blanco Luquin, Idoia
dc.contributor.author	Urdánoz Casado, Amaya
dc.contributor.author	Rubio, Idoya
dc.contributor.author	Labarga Gutiérrez, Alberto
dc.contributor.department	Estadística, Informática y Matemáticas	es_ES
dc.contributor.department	Estatistika, Informatika eta Matematika	eu
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.date.accessioned	2020-08-06T08:35:32Z
dc.date.available	2020-08-06T08:35:32Z
dc.date.issued	2020
dc.description.abstract	Background: The discovery of novel biomarkers of stroke etiology would be most helpful in management of acute ischemic stroke patients. Recently, circular RNAs (circRNAs) have been proposed as candidate biomarkers of neurological conditions due to its high stability. circRNAs function as sponges, sequestering miRNAs and are involved in most relevant biological functions. Our aim was to identify differentially expressed circRNAs in acute ischemic stroke patients according to stroke etiology. Methods: A comprehensive expression profile of blood circRNAs was conducted by Arraystar Human circRNA arrays (13,617 probes) on a discovery cohort of 30 stroke patients with different stroke etiologies by TOAST classification. Real-time quantitative PCR (RT-qPCR) was used to validate array results in a cohort of 50 stroke patients. Functional in silico analysis was performed to identify potential interactions with microRNAs (miRNAs) and pathways underlying deregulated circRNAs. Results: A set of 60 circRNAs were found to be upregulated in atherotrombotic versus cardioembolic strokes (fold-change > = 1.5 and p-value ≤ 0.05). Differential expression of hsa_circRNA_102488, originated from UBA52 gene, was replicated in the validation cohort. RNA-binding proteins (RBPs) sites of hsa_circRNA_102488 clustered around AGO2 and FUS proteins. Further functional analysis revealed interactions between deregulated circRNAs and a set of miRNAs involved in stroke-related pathways, such as fatty acid biogenesis or lysine degradation. Conclusion: Different stroke subtypes show specific profiles of circRNAs expression. circRNAs may serve as a new source of biomarkers of stroke etiology in acute ischemic stroke patients.	en
dc.description.sponsorship	This work was supported by Navarre Government Funding (Industry and Health department) through ADITECH and by RED INVICTUS (RD16/0019/0024) from the Institute of Health Carlos III, jointly funded by European Regional Development Fund (ERDF), European Union. AUC received a grant 'Doctorados industriales 2018–2020' founded by Government of Navarra and MM received a grant 'Programa de intensificación' founded by 'LaCaixa Foundation' and Fundación Caja-Navarra.	en
dc.format.extent	12 p.
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1186/s13578-020-00394-3
dc.identifier.issn	2045-3701
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/37751
dc.language.iso	eng	en
dc.publisher	BioMed Central	en
dc.relation.ispartof	Cell and Bioscience, 2020, 10:34	en
dc.relation.publisherversion	https://doi.org/10.1186/s13578-020-00394-3
dc.rights	© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Stroke	en
dc.subject	Etiology	en
dc.subject	circRNA	en
dc.subject	miRNA	en
dc.subject	Biomarker	en
dc.subject	UBA52	en
dc.title	Circular RNA expression profile in blood according to ischemic stroke etiology	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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