Protein A-mediated multicellular behavior in Staphylococcus aureus
| dc.contributor.author | Merino Barberá, Nekane | |
| dc.contributor.author | Toledo Arana, Alejandro | |
| dc.contributor.author | Vergara Irigaray, Marta | |
| dc.contributor.author | Valle Turrillas, Jaione | |
| dc.contributor.author | Solano Goñi, Cristina | |
| dc.contributor.author | Calvo, Enrique | |
| dc.contributor.author | Lopez, Juan Antonio | |
| dc.contributor.author | Foster, Timothy J. | |
| dc.contributor.author | Penadés, José R. | |
| dc.contributor.author | Lasa Uzcudun, Íñigo | |
| dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es_ES |
| dc.date.accessioned | 2019-01-23T11:16:21Z | |
| dc.date.available | 2019-01-23T11:16:21Z | |
| dc.date.issued | 2008 | |
| dc.description.abstract | The capacity of Staphylococcus aureus to form biofilms on host tissues and implanted medical devices is one of the major virulence traits underlying persistent and chronic infections. The matrix in which S. aureus cells are encased in a biofilm often consists of the polysaccharide intercellular adhesin (PIA) or poly-N-acetyl glucosamine (PNAG). However, surface proteins capable of promoting biofilm development in the absence of PIA/PNAG exopolysaccharide have been described. Here, we used two-dimensional nano-liquid chromatography and mass spectrometry to investigate the composition of a proteinaceous biofilm matrix and identified protein A (spa) as an essential component of the biofilm; protein A induced bacterial aggregation in liquid medium and biofilm formation under standing and flow conditions. Exogenous addition of synthetic protein A or supernatants containing secreted protein A to growth media induced biofilm development, indicating that protein A can promote biofilm development without being covalently anchored to the cell wall. Protein A-mediated biofilm formation was completely inhibited in a dose-dependent manner by addition of serum, purified immunoglobulin G, or anti-protein A-specific antibodies. A murine model of subcutaneous catheter infection unveiled a significant role for protein A in the development of biofilm-associated infections, as the amount of protein A-deficient bacteria recovered from the catheter was significantly lower than that of wild-type bacteria when both strains were used to coinfect the implanted medical device. Our results suggest a novel role for protein A complementary to its known capacity to interact with multiple immunologically important eukaryotic receptors. | en |
| dc.description.sponsorship | This work was supported by the BIO2005-08399 and ERA-Net pathogenomics GEN2006-27792-C2-1-E/PAT grants from the Spanish Ministerio de Educación y Ciencia and grant LSHM-CT-2006-019064 from the European Union. | en |
| dc.format.extent | 12 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1128/jb.01222-08 | |
| dc.identifier.issn | 0021-9193(Print) | |
| dc.identifier.issn | 1098-5530 (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/32086 | |
| dc.language.iso | eng | en |
| dc.publisher | American Society for Microbiology | en |
| dc.relation.ispartof | Journal of Bacteriology, vol. 191, nº 3, feb. 2009, p. 832–843 | en |
| dc.relation.publisherversion | https://doi.org/10.1128/jb.01222-08 | |
| dc.rights | © 2009, American Society for Microbiology. All Rights Reserved. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.subject | Staphylococcus aureus | en |
| dc.subject | Staphylococcal protein | en |
| dc.subject | Multicellular behavior | en |
| dc.subject | Biofilms | en |
| dc.title | Protein A-mediated multicellular behavior in Staphylococcus aureus | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
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