Coping with environmental eukaryotes; identification of Pseudomonas syringae genes during the interaction with alternative hosts or predators

Dorati, Federico; Barrett, Glyn A.; Sánchez Contreras, María; Murillo Martínez, Jesús; Caballero Murillo, Primitivo

Publication:
Coping with environmental eukaryotes; identification of Pseudomonas syringae genes during the interaction with alternative hosts or predators

dc.contributor.author	Dorati, Federico
dc.contributor.author	Barrett, Glyn A.
dc.contributor.author	Sánchez Contreras, María
dc.contributor.author	Murillo Martínez, Jesús
dc.contributor.author	Caballero Murillo, Primitivo
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2020-12-02T10:17:53Z
dc.date.available	2020-12-02T10:17:53Z
dc.date.issued	2018
dc.description.abstract	Understanding the molecular mechanisms underpinning the ecological success of plant pathogens is critical to develop strategies for controlling diseases and protecting crops. Recent observations have shown that plant pathogenic bacteria, particularly Pseudomonas, exist in a range of natural environments away from their natural plant host e.g., water courses, soil, non-host plants. This exposes them to a variety of eukaryotic predators such as nematodes, insects and amoebae present in the environment. Nematodes and amoeba in particular are bacterial predators while insect herbivores may act as indirect predators, ingesting bacteria on plant tissue. We therefore postulated that bacteria are probably under selective pressure to avoid or survive predation and have therefore developed appropriate coping mechanisms. We tested the hypothesis that plant pathogenic Pseudomonas syringae are able to cope with predation pressure and found that three pathovars show weak, but significant resistance or toxicity. To identify the gene systems that contribute to resistance or toxicity we applied a heterologous screening technique, called Rapid Virulence Annotation (RVA), for anti-predation and toxicity mechanisms. Three cosmid libraries for P. syringae pv. aesculi, pv. tomato and pv. phaseolicola, of approximately 2000 cosmids each, were screened in the susceptible/non-toxic bacterium Escherichia coli against nematode, amoebae and an insect. A number of potential conserved and unique genes were identified which included genes encoding haemolysins, biofilm formation, motility and adhesion. These data provide the first multi-pathovar comparative insight to how plant pathogens cope with different predation pressures and infection of an insect gut and provide a foundation for further study into the function of selected genes and their role in ecological success.	en
dc.description.sponsorship	All work was funded by the University of Reading.	en
dc.format.extent	23 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.3390/microorganisms6020032
dc.identifier.issn	2076-2607
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/38818
dc.language.iso	eng	en
dc.publisher	MDPI	en
dc.relation.ispartof	Microorganisms, 2018, 6(2): 32	en
dc.relation.publisherversion	https://doi.org/10.3390/microorganisms6020032
dc.rights	© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access_x000D_ article distributed under the terms and conditions of the Creative Commons Attribution_x000D_ (CC BY) license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Pseudomonas syringae	en
dc.subject	Rapid virulence annotation	en
dc.subject	RVA	en
dc.subject	Pathogen	en
dc.subject	Anti-predation	en
dc.subject	Caenorhabditis elegans	en
dc.subject	Acanthamoeba polyphaga	en
dc.subject	Galleria mellonella	en
dc.title	Coping with environmental eukaryotes; identification of Pseudomonas syringae genes during the interaction with alternative hosts or predators	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dspace.entity.type	Publication
relation.isAuthorOfPublication	3f350c70-2e68-4b9b-95d6-26e9cc1540a0
relation.isAuthorOfPublication	ecde8e03-14c2-46a3-9e89-ae3b2c668297
relation.isAuthorOfPublication.latestForDiscovery	3f350c70-2e68-4b9b-95d6-26e9cc1540a0