Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA

Sanjosé, Leticia; Crespo, Helena; Glaría Ezquer, Idoia; Andrés Cara, Damián de; Reina Arias, Ramsés

Publication:
Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA

dc.contributor.author	Sanjosé, Leticia	es_ES
dc.contributor.author	Crespo, Helena
dc.contributor.author	Glaría Ezquer, Idoia
dc.contributor.author	Andrés Cara, Damián de
dc.contributor.author	Reina Arias, Ramsés
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2020-09-16T11:21:48Z
dc.date.available	2020-09-16T11:21:48Z
dc.date.issued	2015
dc.description.abstract	The major challenges in diagnosing small ruminant lentivirus (SRLV) infection include early detection and genotyping of strains of epidemiological interest. A longitudinal study was carried out in Rasa Aragonesa sheep experimentally infected with viral strains of genotypes A or B from Spanish neurological and arthritic SRLV outbreaks, respectively. Sera were tested with two commercial ELISAs, three based on specific peptides and a novel combined peptide ELISA. Three different PCR assays were used to further assess infection status. The kinetics of anti-viral antibody responses were variable, with early diagnosis dependent on the type of ELISA used. Peptide epitopes of SRLV genotypes A and B combined in the same ELISA well enhanced the overall detection rate, whereas single peptides were useful for genotyping the infecting strain (A vs. B). The results of the study suggest that a combined peptide ELISA can be used for serological diagnosis of SRLV infection, with single peptide ELISAs useful for subsequent serotyping.	en
dc.description.sponsorship	Funded by CICYT (AGL2010-22341-C04-01 and AGL2013-49137-C3-1R) and Navarra’s Government (IIQ010449.RI1 and IIQ14064.RI1). L. Sanjosé was a FPI-fellow of the Spanish MINECO and R. Reina had a contract of the Public University of Navarra.	en
dc.format.extent	28 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1016/j.tvjl.2015.01.012
dc.identifier.issn	1090-0233
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/38131
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	The Veterinary Journal, 2015, 204, 88-93	en
dc.relation.projectID	info:eu-repo/grantAgreement/MINECO//AGL2013-49137-C3-1-R/ES/	en
dc.relation.publisherversion	https://doi.org/10.1016/j.tvjl.2015.01.012
dc.rights	© 2015 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Small ruminant lentivirus	en
dc.subject	Genotype	en
dc.subject	Serology	en
dc.subject	Peptide ELISA	en
dc.title	Diagnosing infection with small ruminant lentiviruses of genotypes A and B by combining synthetic peptides in ELISA	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dspace.entity.type	Publication
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