Role of bacterial surface structures on the interaction of Klebsiella pneumoniae with phagocytes

March, Catalina; Cano, Victoria; Moranta, David; Llobet, Enrique; Pérez Gutiérrez, Camino; Tomás, Juan M.; Suárez, Teresa; Garmendia García, Juncal; Bengoechea Alonso, José Antonio

Role of bacterial surface structures on the interaction of Klebsiella pneumoniae with phagocytes

dc.contributor.author	March, Catalina
dc.contributor.author	Cano, Victoria
dc.contributor.author	Moranta, David
dc.contributor.author	Llobet, Enrique
dc.contributor.author	Pérez Gutiérrez, Camino
dc.contributor.author	Tomás, Juan M.
dc.contributor.author	Suárez, Teresa
dc.contributor.author	Garmendia García, Juncal
dc.contributor.author	Bengoechea Alonso, José Antonio
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2018-09-06T12:20:24Z
dc.date.available	2018-09-06T12:20:24Z
dc.date.issued	2013
dc.description.abstract	Phagocytosis is a key process of the immune system. The human pathogen Klebsiella pneumoniae is a well known example of a pathogen highly resistant to phagocytosis. A wealth of evidence demonstrates that the capsule polysaccharide (CPS) plays a crucial role in resistance to phagocytosis. The amoeba Dictyostelium discoideum shares with mammalian macrophages the ability to phagocytose and kill bacteria. The fact that K. pneumoniae is ubiquitous in nature and, therefore, should avoid predation by amoebae, poses the question whether K. pneumoniae employs similar means to counteract amoebae and mammalian phagocytes. Here we developed an assay to evaluate K. pneumoniae-D. discoideum interaction. The richness of the growth medium affected the threshold at which the cps mutant was permissive for Dictyostelium and only at lower nutrient concentrations the cps mutant was susceptible to predation by amoebae. Given the critical role of bacterial surface elements on host-pathogen interactions, we explored the possible contribution of the lipopolysaccharide (LPS) and outer membrane proteins (OMPs) to combat phagoyctosis by D. discoideum. We uncover that, in addition to the CPS, the LPS O-polysaccharide and the first core sugar participate in Klebsiella resistance to predation by D. discoideum. K. pneumoniae LPS lipid A decorations are also necessary to avoid predation by amoebae although PagP-dependent palmitoylation plays a more important role than the lipid A modification with aminoarabinose. Mutants lacking OMPs OmpA or OmpK36 were also permissive for D. discoideium growth. Except the LPS O-polysaccharide mutants, all mutants were more susceptible to phagocytosis by mouse alveolar macrophages. Finally, we found a correlation between virulence, using the pneumonia mouse model, and resistance to phagocytosis. Altogether, this work reveals novel K. pneumoniae determinants involved in resistance to phagocytosis and supports the notion that Dictyostelium amoebae might be useful as host model to measure K. pneumoniae virulence and not only phagocytosis.	en
dc.description.sponsorship	Part of this work was supported by Ministerio de Economía y Competitividad (Spain) and from Generalitat de Catalunya (Centre de Referencia en Biotecnologia) grants to J.M.T.; by grants from Biomedicine Program (SAF2009-07885; Ministerio de Economía y Competitividad) and SEPAR (Sociedad Española de Neumología, project reference 068/2011) to J.A.B. The laboratory of Microbial Pathogenesis is supported by a grant to Competitive groups (project reference 46/2011) from Govern Illes Balears (co-funded by European Regional Development Fund).	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.doi	10.1371/journal.pone.0056847
dc.identifier.issn	1932-6203
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/30559
dc.language.iso	eng	en
dc.publisher	Public Library of Science	en
dc.relation.ispartof	Plos One, 8(2): e56847	en
dc.relation.publisherversion	https://doi.org/10.1371/journal.pone.0056847
dc.rights	© 2013 March et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/3.0/
dc.subject	Phagocytosis	en
dc.subject	Klebsiella pneumoniae	en
dc.subject	Dictyostelium discoideum	en
dc.subject	Bacterial surface structures	en
dc.title	Role of bacterial surface structures on the interaction of Klebsiella pneumoniae with phagocytes	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication