Plastidial phosphoglucose isomerase is an important determinant of seed yield through its involvement in gibberellin-mediated reproductive development and storage reserve biosynthesis in arabidopsis

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dc.contributor.authorBahaji, Abdellatif
dc.contributor.authorAlmagro Zabalza, Goizeder
dc.contributor.authorEzquer, Ignacio
dc.contributor.authorGámez Arcas, Samuel
dc.contributor.authorSánchez López, Ángela María
dc.contributor.authorMuñoz Pérez, Francisco José
dc.contributor.authorBarrio, Ramón José
dc.contributor.authorSampedro, M. Carmen
dc.contributor.authorDiego, Nuria de
dc.contributor.authorSpíchal, Lukás
dc.contributor.authorDolezal, Karel
dc.contributor.authorTarkowská, Danuse
dc.contributor.authorCaporali, Elisabetta
dc.contributor.authorMendes, Marta Adelina
dc.contributor.authorBaroja Fernández, Edurne
dc.contributor.authorPozueta Romero, Javier
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua, ref. P1004 PROMEBIOes
dc.date.accessioned2019-08-26T11:13:29Z
dc.date.available2019-08-26T11:13:29Z
dc.date.issued2018
dc.description.abstractThe plastid-localized phosphoglucose isomerase isoform PGI1 is an important determinant of growth in Arabidopsis thaliana, likely due to its involvement in the biosynthesis of plastidial isoprenoid-derived hormones. Here, we investigated whether PGI1 also influences seed yields. PGI1 is strongly expressed in maturing seed embryos and vascular tissues. PGI1-null pgi1-2 plants had ∼60% lower seed yields than wild-type plants, with reduced numbers of inflorescences and thus fewer siliques and seeds per plant. These traits were associated with low bioactive gibberellin (GA) contents. Accordingly, wild-type phe-notypes were restored by exogenous GA application. pgi1-2 seeds were lighter and accumulated ∼50% less fatty acids (FAs) and ∼35% less protein than wild-type seeds. Seeds of cytokinin-deficient plants overexpressing CYTOKININ OXIDASE/DE-HYDROGENASE1 (35S:AtCKX1) and GA-deficient ga20ox1 ga20ox2 mutants did not accumulate low levels of FAs, and exogenous application of the cytokinin 6-benzylaminopurine and GAs did not rescue the reduced weight and FA content of pgi1-2 seeds. Seeds from reciprocal crosses between pgi1-2 and wild-type plants accumulated wild-type levels of FAs and proteins. Therefore, PGI1 is an important determinant of Arabidopsis seed yield due to its involvement in two processes: GA-mediated reproductive development and the metabolic conversion of plastidial glucose-6-phosphate to storage reserves in the embryo.en
dc.description.sponsorshipThis work was partially supported by the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) (grant numbers BIO2013-49125-C2-1-P and BIO2016-78747-P), the Ministry of Education, Youth and Sports of the Czech Republic (grant LO1204 from the National Program of Sustainability I), the Government of Navarra (ref. P1004 PROMEBIO), the Università degli Studi di Milano (UNIMI-RTD-A, Linea2-DBS 2017-2018) and the H2020-MSCA-RISE project (ExpoSeed GA-691109).en
dc.format.extent58 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1105/tpc.18.00312
dc.identifier.issn1040-4651
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/34671
dc.language.isoengen
dc.publisherAmerican Society of Plant Biologistsen
dc.relation.ispartofPlant Cell, 30 (9), 2082-2098en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BIO2013-49125-C2-2-P/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/BIO2016-78747-P/
dc.relation.publisherversionhttps://doi.org/10.1105/tpc.18.00312
dc.rights© 2018 American Society of Plant Biologists. All rights reserved.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectPlastidial phosphoglucose isomeraseen
dc.subjectArabidopsis thalianaen
dc.subjectSeed yieldsen
dc.subjectGibberellinen
dc.subjectFatty acidsen
dc.subjectWild type plantsen
dc.titlePlastidial phosphoglucose isomerase is an important determinant of seed yield through its involvement in gibberellin-mediated reproductive development and storage reserve biosynthesis in arabidopsisen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublication
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relation.isAuthorOfPublication041a1a7f-8b33-491a-8a52-015063f9b1ae
relation.isAuthorOfPublicationfe94cbef-f6fb-4757-93d4-7ec184ca08ef
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relation.isAuthorOfPublication.latestForDiscovery98c9658b-9c03-41bf-9966-776fa23ec157

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