Ectopic expression of the AtCDF1 transcription factor in potato enhances tuber starch and amino acid contents and yield under open field conditions

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dc.contributor.authorCarrillo, Laura
dc.contributor.authorBaroja Fernández, Edurne
dc.contributor.authorRenau Morata, Begoña
dc.contributor.authorMuñoz Pérez, Francisco José
dc.contributor.authorCanales, Javier
dc.contributor.authorCiordia, Sergio
dc.contributor.authorYang, Lu
dc.contributor.authorSánchez López, Ángela María
dc.contributor.authorNebauer, Sergio G.
dc.contributor.authorCeballos, Mar G.
dc.contributor.authorVicente-Carbajosa, Jesús
dc.contributor.authorMolina, Rosa V.
dc.contributor.authorPozueta Romero, Javier
dc.contributor.authorMedina, Joaquín
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2023-08-24T11:10:05Z
dc.date.available2023-08-24T11:10:05Z
dc.date.issued2023
dc.date.updated2023-08-24T10:49:46Z
dc.description.abstractIntroduction: cycling Dof transcription factors (CDFs) have been involved in different aspects of plant growth and development. In Arabidopsis and tomato, one member of this family (CDF1) has recently been associated with the regulation of primary metabolism and abiotic stress responses, but their roles in crop production under open field conditions remain unknown. Methods: in this study, we compared the growth, and tuber yield and composition of plants ectopically expressing the CDF1 gene from Arabidopsis under the control of the 35S promoter with wild-type (WT) potato plants cultured in growth chamber and open field conditions. Results: in growth chambers, the 35S::AtCDF1 plants showed a greater tuber yield than the WT by increasing the biomass partition for tuber development. Under field conditions, the ectopic expression of CDF1 also promoted the sink strength of the tubers, since 35S::AtCDF1 plants exhibited significant increases in tuber size and weight resulting in higher tuber yield. A metabolomic analysis revealed that tubers of 35S::AtCDF1 plants cultured under open field conditions accumulated higher levels of glucose, starch and amino acids than WT tubers. A comparative proteomic analysis of tubers of 35S::AtCDF1 and WT plants cultured under open field conditions revealed that these changes can be accounted for changes in the expression of proteins involved in energy production and different aspects of C and N metabolism. Discussion: The results from this study advance our collective understanding of the role of CDFs and are of great interest for the purposes of improving the yield and breeding of crop plants.en
dc.description.sponsorshipThis study was supported by the Ministerio de Ciencia e Innovación (MCIN) and the Agencia Estatal de Investigación (AEI) / 10.13039/501100011033/ (grants RTA2015-00014-c02-00 to JM and SGN; PID2020-114165RR-C21 to JM), (BIO2017-82873-R to JVC) (PID2019-104685GB-100 to JP-R, EB-F and FJM), the Gobierno de Navarra (PC036-037 BIOMEF to EB-F and FJM) and the Vicerrectorado de Investigación de la Universitat Politècnica de València (PAID-11-21 to SGN), J.C is funded by the National Agency for Research and Development (ANID) Chile with Program FONDECYT Regular 1190812 and ANID-Millennium Science Initiative Program-ICN17_022" and The GEP Chinese pre-doctoral fellowship to LY. We also want to acknowledge the "Severo Ochoa Program for Centres of Excellence in R&D" (CEX2020-000999-S) supported by MCIN/AEI/10.13039/501100011033 for supporting the scientific services used in this study.en
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dc.identifier.citationCarrillo, L., Baroja-Fernández, E., Renau-Morata, B., Muñoz, F. J., Canales, J., Ciordia, S., Yang, L., Sánchez-López, Á. M., Nebauer, S. G., Ceballos, M. G., Vicente-Carbajosa, J., Molina, R. V., Pozueta-Romero, J., & Medina, J. (2023). Ectopic expression of the AtCDF1 transcription factor in potato enhances tuber starch and amino acid contents and yield under open field conditions. Frontiers in Plant Science, 14, 1010669. https://doi.org/10.3389/fpls.2023.1010669en
dc.identifier.doi10.3389/fpls.2023.1010669
dc.identifier.issn1664-462X
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/46003
dc.language.isoengen
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Plant Science, 14, 1010669en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114165RR-C21/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-82873-R/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104685GB-I00/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/Gobierno de Navarra//PC036-037/
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2023.1010669
dc.rights© 2023 Carrillo, Baroja-Fernández, Renau-Morata, Muñoz, Canales, Ciordia, Yang, Sánchez-López, Nebauer, Ceballos, Vicente-Carbajosa, Molina, Pozueta-Romero and Medina. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAmino aciden
dc.subjectC/N metabolismen
dc.subjectCDFen
dc.subjectCrop yielden
dc.subjectPhotoassimilate partitionen
dc.subjectPotatoen
dc.subjectStarchen
dc.titleEctopic expression of the AtCDF1 transcription factor in potato enhances tuber starch and amino acid contents and yield under open field conditionsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication0a533d7e-81fb-4439-ad05-2927e96fa923
relation.isAuthorOfPublicationfe94cbef-f6fb-4757-93d4-7ec184ca08ef
relation.isAuthorOfPublication80c8bd69-4127-4474-a679-3b10747cae42
relation.isAuthorOfPublication.latestForDiscovery0a533d7e-81fb-4439-ad05-2927e96fa923

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