Calcium inhibits bap-dependent multicellular behavior in Staphylococcus aureus
| dc.contributor.author | Arrizubieta Balerdi, María Jesús | |
| dc.contributor.author | Toledo Arana, Alejandro | |
| dc.contributor.author | Amorena Zabalza, Beatriz | |
| dc.contributor.author | Penadés, José R. | |
| dc.contributor.author | Lasa Uzcudun, Íñigo | |
| dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es_ES |
| dc.contributor.funder | Gobierno de Navarra / Nafarroako Gobernua | es |
| dc.date.accessioned | 2019-01-23T11:16:25Z | |
| dc.date.available | 2019-01-23T11:16:25Z | |
| dc.date.issued | 2004 | |
| dc.description.abstract | Bap (biofilm-associated protein) is a 254-kDa staphylococcal surface protein implicated in formation of biofilms by staphylococci isolated from chronic mastitis infections. The presence of potential EF-hand motifs in the amino acid sequence of Bap prompted us to investigate the effect of calcium on the multicellular behavior of Bap-expressing staphylococci. We found that addition of millimolar amounts of calcium to the growth media inhibited intercellular adhesion of and biofilm formation by Bap-positive strain V329. Addition of manganese, but not addition of magnesium, also inhibited biofilm formation, whereas bacterial aggregation in liquid media was greatly enhanced by metal-chelating agents. In contrast, calcium or chelating agents had virtually no effect on the aggregation of Bap-deficient strain M556. The biofilm elicited by insertion of bap into the chromosome of a biofilm-negative strain exhibited a similar dependence on the calcium concentration, indicating that the observed calcium inhibition was an inherent property of the Bap-mediated biofilms. Site-directed mutagenesis of two of the putative EF-hand domains resulted in a mutant strain that was capable of forming a biofilm but whose biofilm was not inhibited by calcium. Our results indicate that Bap binds Ca2+ with low affinity and that Ca2+ binding renders the protein noncompetent for biofilm formation and for intercellular adhesion. The fact that calcium inhibition of Bap-mediated multicellular behavior takes place in vitro at concentrations similar to those found in milk serum supports the possibility that this inhibition is relevant to the pathogenesis and/or epidemiology of the bacteria in the mastitis process. | en |
| dc.description.sponsorship | This work was supported by the Comisión Interministerial de Ciencia y Tecnología of Spain through grant BIO2002-01841 to M.J.A. and grant BIO2002-04542-C02 to I.L. and J.P. and by the Beca Ortiz de Landazuri award from the Departamento de Salud del Gobierno de Navarra. A.T.-A. is a predoctoral fellow of the Ministerio de Educación, Cultura y Deporte (FPU), Spain. | en |
| dc.format.extent | 9 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1128/jb.186.22.7490-7498.2004 | |
| dc.identifier.issn | 0021-9193(Print) | |
| dc.identifier.issn | 1098-5530 (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/32093 | |
| dc.language.iso | eng | en |
| dc.publisher | American Society for Microbiology | en |
| dc.relation.ispartof | Journal of Bacteriology, vol. 186, nº 22, nov. 2004, p. 7490–7498 | en |
| dc.relation.publisherversion | https://doi.org/10.1128/jb.186.22.7490-7498.2004 | |
| dc.rights | © 2004, American Society for Microbiology. All Rights Reserved. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.subject | Bap (biofilm associated protein) | en |
| dc.subject | Staphylococcus aureus | en |
| dc.subject | Formation of biofilms | en |
| dc.title | Calcium inhibits bap-dependent multicellular behavior in Staphylococcus aureus | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
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