Extraction of phenolic compounds from populus salicaceae bark

Autor, E.; Cornejo Ibergallartu, Alfonso; Bimbela Serrano, Fernando; Maisterra Udi, Maitane; Gandía Pascual, Luis; Martínez-Merino, V.

Publication:
Extraction of phenolic compounds from populus salicaceae bark

dc.contributor.author	Autor, E.	es_ES
dc.contributor.author	Cornejo Ibergallartu, Alfonso
dc.contributor.author	Bimbela Serrano, Fernando
dc.contributor.author	Maisterra Udi, Maitane
dc.contributor.author	Gandía Pascual, Luis
dc.contributor.author	Martínez-Merino, V.	es_ES
dc.contributor.department	Zientziak	eu
dc.contributor.department	Institute for Advanced Materials and Mathematics - INAMAT2	en
dc.contributor.department	Ciencias	es_ES
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate	es
dc.date.accessioned	2022-06-30T11:54:21Z
dc.date.available	2022-06-30T11:54:21Z
dc.date.issued	2022
dc.date.updated	2022-06-30T11:50:43Z
dc.description.abstract	Lignocellulosic residues have the potential for obtaining high value-added products that could be better valorized if biorefinery strategies are adopted. The debarking of short-rotation crops yields important amounts of residues that are currently underexploited as low-grade fuel and could be a renewable source of phenolic compounds and other important phytochemicals. The isolation of these compounds can be carried out by different methods, but for attaining an integral valorization of barks, a preliminary extraction step for phytochemicals should be included. Using optimized extraction methods based on Soxhlet extraction can be effective for the isolation of phenolic compounds with antioxidant properties. In this study, poplar bark (Populus Salicaceae) was used to obtain a series of extracts using five different solvents in a sequential extraction of 24 h each in a Soxhlet extractor. Selected solvents were put in contact with the bark sample raffinate following an increasing order of polarity: n-hexane, dichloromethane, ethyl acetate, methanol, and water. The oily residues of the extracts obtained after each extraction were further subjected to flash chromatography, and the fractions obtained were characterized by gas chromatography coupled with mass spectrometry (GC–MS). The total phenolic content (TPC) was determined using the Folin–Ciocalteu method, and the antioxidant activity (AOA) of the samples was evaluated in their reaction with the free radical 2,2-Diphenyl-picrylhydrazyl (DPPH method). Polar solvents allowed for higher individual extraction yields, with overall extraction yields at around 23% (dry, ash-free basis). Different compounds were identified, including hydrolyzable tannins, phenolic monomers such as catechol and vanillin, pentoses and hexoses, and other organic compounds such as long-chain alkanes, alcohols, and carboxylic acids, among others. An excellent correlation was found between TPC and antioxidant activity for the samples analyzed. The fractions obtained using methanol showed the highest phenolic content (608 g of gallic acid equivalent (GAE)/mg) and the greatest antioxidant activity.	en
dc.description.sponsorship	This research was funded by the Gobierno de Navarra, grant number PC177-178 Reducenano 2.0 and Spanish Ministry of Science (AbFine, PID2020-114936RB-I00). The APC was funded by the Universidad Pública de Navarra (UPNA).	en
dc.format.mimetype	application/pdf	en
dc.format.mimetype	application/zip	en
dc.identifier.citation	Autor, E.; Cornejo-Ibergallartu, A.; Bimbela-Serrano, F.; Maisterra-Udi, M.; Gandía-PascualE, L. M.; Martínez-Merino, V.. (2022). Extraction of phenolic compounds from populus salicaceae bark. Biomolecules. 12,4 .	es
dc.identifier.doi	10.3390/biom12040539
dc.identifier.issn	2218-273X
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/43276
dc.language.iso	eng	en
dc.publisher	MDPI	en
dc.relation.ispartof	Biomolecules, 2022, 12 (4), p. 539	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114936RB-I00/ES/	en
dc.relation.publisherversion	https://doi.org/10.3390/biom12040539
dc.rights	© 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject	Antioxidants	en
dc.subject	Tannins	en
dc.subject	Phenolics	en
dc.subject	Soxhlet	en
dc.subject	Folin-Ciocalteu	en
dc.subject	2,2-Diphenyl-picrylhydrazyl	en
dc.subject	DPPH	en
dc.title	Extraction of phenolic compounds from populus salicaceae bark	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión publicada / Argitaratu den bertsioa	es
dc.type.version	info:eu-repo/semantics/publishedVersion	en
dspace.entity.type	Publication
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