Methods for analysis of specific DNA methylation status

Pajares Villandiego, María Josefa; Palanca Ballester, Cora; Urtasun Alonso, Raquel; Alemany Cosme, Ester; Lahoz, Agustín; Sandoval, Juan

Publication:
Methods for analysis of specific DNA methylation status

dc.contributor.author	Pajares Villandiego, María Josefa
dc.contributor.author	Palanca Ballester, Cora
dc.contributor.author	Urtasun Alonso, Raquel
dc.contributor.author	Alemany Cosme, Ester
dc.contributor.author	Lahoz, Agustín
dc.contributor.author	Sandoval, Juan
dc.contributor.department	Ciencias de la Salud	es_ES
dc.contributor.department	Osasun Zientziak	eu
dc.date.accessioned	2021-04-23T07:56:46Z
dc.date.available	2022-03-01T00:00:15Z
dc.date.issued	2021
dc.description.abstract	Methylation of CpG dinucleotides plays a crucial role in the regulation of gene expression and therefore in the development of different pathologies. Aberrant methylation has been associated to the majority of the diseases, including cancer, neurodegenerative, cardiovascular and autoimmune disorders. Analysis of DNA methylation patterns is crucial to understand the underlying molecular mechanism of these diseases. Moreover, DNA methylation patterns could be used as biomarker for clinical management, such as diagnosis, prognosis and treatment response. Nowadays, a variety of high throughput methods for DNA methylation have been developed to analyze the methylation status of a high number of CpGs at once or even the whole genome. However, identification of specific methylation patterns at specific loci is essential for validation and also as a tool for diagnosis. In this review, we describe the most commonly used approaches to evaluate specific DNA methylation. There are three main groups of techniques that allow the identification of specific regions that are differentially methylated: bisulfite conversion-based methods, restriction enzyme-based approaches, and affinity enrichment-based assays. In the first group, specific restriction enzymes recognize and cleave unmethylated DNA, leaving methylated sequences intact. Bisulfite conversion methods are the most popular approach to distinguish methylated and unmethylated DNA. Unmethylated cytosines are deaminated to uracil by sodium bisulfite treatment, while the methyl cytosines remain unconverted. In the last group, proteins with methylation binding domains or antibodies against methyl cytosines are used to recognize methylated DNA. In this review, we provide the theoretical basis and the framework of each technique as well as the analysis of their strength and the weaknesses.	en
dc.embargo.lift	2022-03-01
dc.embargo.terms	2022-03-01
dc.format.extent	31 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1016/j.ymeth.2020.06.021
dc.identifier.issn	1046-2023
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/39567
dc.language.iso	eng	en
dc.publisher	Elevier	en
dc.relation.ispartof	Methods 187 (2021) 3-12	en
dc.relation.publisherversion	https://doi.org/10.1016/j.ymeth.2020.06.021
dc.rights	© 2020 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Epigenetics	en
dc.subject	DNA methylation	en
dc.subject	Target region	en
dc.subject	Locus specific analyses	en
dc.subject	Bisulfite conversion	en
dc.subject	Digital PCR	en
dc.subject	CpG islands	en
dc.title	Methods for analysis of specific DNA methylation status	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dspace.entity.type	Publication
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relation.isAuthorOfPublication.latestForDiscovery	601df8fd-4b6f-47c4-be5d-4b81d2c1cf64