Femtomolar detection by nanocoated fiber label-free biosensors

Chiavaioli, Francesco; Zubiate Orzanco, Pablo; Del Villar, Ignacio; Ruiz Zamarreño, Carlos; Giannetti, Ambra; Tombelli, Sara; Trono, Cosimo; Arregui San Martín, Francisco Javier; Matías Maestro, Ignacio; Baldini, Francesco

Publication:
Femtomolar detection by nanocoated fiber label-free biosensors

dc.contributor.author	Chiavaioli, Francesco
dc.contributor.author	Zubiate Orzanco, Pablo
dc.contributor.author	Del Villar, Ignacio
dc.contributor.author	Ruiz Zamarreño, Carlos
dc.contributor.author	Giannetti, Ambra
dc.contributor.author	Tombelli, Sara
dc.contributor.author	Trono, Cosimo
dc.contributor.author	Arregui San Martín, Francisco Javier
dc.contributor.author	Matías Maestro, Ignacio
dc.contributor.author	Baldini, Francesco
dc.contributor.department	Institute of Smart Cities - ISC	en
dc.contributor.department	Ingeniería Eléctrica y Electrónica	es_ES
dc.contributor.department	Ingeniaritza Elektrikoa eta Elektronikoa	eu
dc.contributor.funder	Gobierno de Navarra / Nafarroako Gobernua	es
dc.date.accessioned	2024-06-23T18:52:16Z
dc.date.available	2024-01-12T13:15:08Z
dc.date.issued	2018
dc.date.updated	2024-01-12T13:03:07Z
dc.description.abstract	The advent of optical fibre-based biosensors combined with that of nanotechnologies has provided an oppor-tunity for developing in situ, portable, lightweight, versatile and high-performance optical sensing platforms. We report on the generation of lossy mode resonances by the deposition of nm-thick metal oxide films on optical fibres, which makes it possible to measure precisely and accurately the changes in optical properties of the fibre-surrounding medium with very high sensitivity compared to other technology platforms, such as long period gratings or surface plasmon resonances, the gold standard in label-free and real-time biomolecular interaction analysis. This property, combined with the application of specialty structures such as D-shaped fibres, permits enhancing the light-matter interaction. SEM and TEM imaging together with X-EDS tool have been utilised to characterise the two films used, i.e. indium tin oxide and tin dioxide. More-over, the experimental transmission spectra obtained after the deposition of the nanocoatings have been numerically cor-roborated by means of wave propagation methods. With the use of a conventional wavelength interrogation system and ad-hoc developed microfluidics, the shift of the lossy mode resonance can be reliably recorded in response to very low analyte concentrations. Repeated experiments confirm a big leap in performance thanks to the capability to detect femtomolar concentrations in human serum, improving the detection limit by three orders of magnitude when compared with other fibre-based configurations. The biosensor has been regenerated several times by injecting sodium dodecyl sul-phate, which proves the capability of sensor to be reused.	en
dc.description.sponsorship	This work was supported by the European Community for theproject Hemospec (FP7-611682), by the National ResearchCouncil of Italy (CNR) for the Short Term Mobility program2017, by the Government of Navarra (project no. 72/2015)and by the Spanish Agencia Estatal de Investigación (AEI) andEuropean Regional Development Fund (FEDER) (TEC2016-78047-R, TEC2016-79367-C2-2-R).	en
dc.format.mimetype	application/pdf	en
dc.identifier.citation	Chiavaioli, F., Zubiate, P., Del Villar, I., Zamarreño, C. R., Giannetti, A., Tombelli, S., Trono, C., Arregui, F. J., Matias, I. R., & Baldini, F. (2018). Femtomolar detection by nanocoated fiber label-free biosensors. ACS Sensors, 3(5), 936-943. https://doi.org/10.1021/acssensors.7b00918	en
dc.identifier.doi	10.1021/acssensors.7b00918
dc.identifier.issn	2379-3694
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/47017
dc.language.iso	eng	en
dc.publisher	ACS	en
dc.relation.ispartof	ACS Sensors, 2018, 3, 936-943	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI//TEC2016-78047-R	en
dc.relation.projectID	info:eu-repo/grantAgreement/AEI//TEC2016-79367-C2-2-R	en
dc.relation.projectID	info:eu-repo/grantAgreement/Gobierno de Navarra//72%2F2015	en
dc.relation.publisherversion	https://doi.org/10.1021/acssensors.7b00918
dc.rights	© 2018 American Chemical Society.	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.subject	Optical biosensor	en
dc.subject	Lossy mode resonance	en
dc.subject	Optical fiber sensor	en
dc.subject	Nanometric metal oxide film	en
dc.subject	Label-free biomolecular interaction	en
dc.subject	Femtomolar detection limit	en
dc.subject	Regeneration	en
dc.title	Femtomolar detection by nanocoated fiber label-free biosensors	en
dc.type	Artículo / Artikulua	es
dc.type	info:eu-repo/semantics/article	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dspace.entity.type	Publication
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