Hybrid pine (Pinus attenuata × Pinus radiata) somatic embryogenesis: what do you prefer, mother or nurse?

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dc.contributor.authorMontalbán, Itziar A.
dc.contributor.authorCastander Olarieta, Ander
dc.contributor.authorHargreaves, Cathy L.
dc.contributor.authorGough, Keiko
dc.contributor.authorReeves, Cathie B.
dc.contributor.authorBallekom, Shaf van
dc.contributor.authorGoicoa Mangado, Tomás
dc.contributor.authorUgarte Martínez, María Dolores
dc.contributor.authorMoncaleán, Paloma
dc.contributor.departmentEstatistika, Informatika eta Matematikaeu
dc.contributor.departmentInstitute for Advanced Materials and Mathematics - INAMAT2en
dc.contributor.departmentEstadística, Informática y Matemáticases_ES
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2021-06-23T06:38:11Z
dc.date.available2021-06-23T06:38:11Z
dc.date.issued2021
dc.description.abstractDevelopment of hybrid pines of Pinus radiata D. Don for commercial forestry presents an opportunity to diversify the current resource of plant material. Climate change and different land uses pose challenges, making alternative species necessary to guarantee wood and non-wood products in the future. Pinus radiata var. cedrosensis × Pinus attenuata hybrid possesses different attributes, such as tolerance to drought conditions, better growth and resistance to snow damage at higher altitudes, and more importantly, different wood quality characteristics. Embryogenic cell lines were successfully initiated reciprocal hybrids using as initial explants megagametophytes, excised zygotic embryos and excised zygotic embryos plus nurse culture. However, the questions raised were: does the initiation environment affect the conversion to somatic plantlets months later? Does the mother tree or the cross have an effect on the conversion to somatic plantlets? In the present work we analysed the maturation rate, number of somatic embryos, germination rate, and the ex-vitro growth in cell lines derived from different initiation treatments, mother tree species, and crosses. Differences were not observed for in vitro parameters such as maturation and germination. However, significant differences were observed due to the mother tree species in relation with the ex-vitro growth rates observed, being higher those in which P. radiata acted as a mother. Moreover, embryogenic cell lines from these hybrids were stored at −80◦C and regenerated after one and five years.en
dc.description.sponsorshipThis research was funded by MINECO (Spanish Government) project (AGL2016-76143-C4-3R), CYTED (P117RT0522), DECO (Basque government, 'Ayudas de formación a jóvenes investi-gadores y tecnólogos'). OECD Co-operative Research Programme Fellowship (Biological Resource Management for Sustainable Agricultural Systems, 2013) for supporting the visit of Paloma Moncaleán to Scion and Scion Core Funding for supporting the hybrid pine initiative.
dc.format.extent12 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.3390/f12010045
dc.identifier.issn1999-4907
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/40029
dc.language.isoengen
dc.publisherMDPI
dc.relation.ispartofForests, 2021, 12, 45
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-76143-C4-3R/
dc.relation.publisherversionhttps://doi.org/10.3390/f12010045
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectEmbryogenic cell linesen
dc.subjectEmbryonal massesen
dc.subjectHybrid pineen
dc.subjectPlantletsen
dc.subjectRadiata pineen
dc.subjectSomatic embryogenesisen
dc.subjectSomatic embryosen
dc.titleHybrid pine (Pinus attenuata × Pinus radiata) somatic embryogenesis: what do you prefer, mother or nurse?en
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication77ba75f3-a30d-4f01-8cc5-9de6d3a10d8d
relation.isAuthorOfPublicatione87ff19e-9d36-4286-989b-cafd391dff9d
relation.isAuthorOfPublication.latestForDiscovery77ba75f3-a30d-4f01-8cc5-9de6d3a10d8d

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