Plastidic phosphoglucose isomerase is an important determinant of starch accumulation in mesophyll cells, growth, photosynthetic capacity, and biosynthesis of plastidic cytokinins in Arabidopsis

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dc.contributor.authorBahaji, Abdellatif
dc.contributor.authorSánchez López, Ángela María
dc.contributor.authorDiego, Nuria de
dc.contributor.authorMuñoz Pérez, Francisco José
dc.contributor.authorBaroja Fernández, Edurne
dc.contributor.authorLi, Jun
dc.contributor.authorRicarte Bermejo, Adriana
dc.contributor.authorBaslam, Marouane
dc.contributor.authorAranjuelo Michelena, Iker
dc.contributor.authorAlmagro Zabalza, Goizeder
dc.contributor.authorHumplik, Jan F.
dc.contributor.authorNovák, Ondrej
dc.contributor.authorSpíchal, Lukás
dc.contributor.authorDolezal, Karel
dc.contributor.authorPozueta Romero, Javier
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua, IIM010491.RI2es
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2018-09-06T12:20:18Z
dc.date.available2018-09-06T12:20:18Z
dc.date.issued2015
dc.description.abstractPhosphoglucose isomerase (PGI) catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. It is involved in glycolysis and in the regeneration of glucose-6-P molecules in the oxidative pentose phosphate pathway (OPPP). In chloroplasts of illuminated mesophyll cells PGI also connects the Calvin-Benson cycle with the starch biosynthetic pathway. In this work we isolated pgi1-3, a mutant totally lacking pPGI activity as a consequence of aberrant intron splicing of the pPGI encoding gene, PGI1. Starch content in pgi1-3 source leaves was ca. 10-15% of that of wild type (WT) leaves, which was similar to that of leaves of pgi1-2, a T-DNA insertion pPGI null mutant. Starch deficiency of pgi1 leaves could be reverted by the introduction of a sex1 null mutation impeding β-amylolytic starch breakdown. Although previous studies showed that starch granules of pgi1-2 leaves are restricted to both bundle sheath cells adjacent to the mesophyll and stomata guard cells, microscopy analyses carried out in this work revealed the presence of starch granules in the chloroplasts of pgi1-2 and pgi1-3 mesophyll cells. RT-PCR analyses showed high expression levels of plastidic and extra-plastidic β-amylase encoding genes in pgi1 leaves, which was accompanied by increased β-amylase activity. Both pgi1-2 and pgi1-3 mutants displayed slow growth and reduced photosynthetic capacity phenotypes even under continuous light conditions. Metabolic analyses revealed that the adenylate energy charge and the NAD(P)H/NAD(P) ratios in pgi1 leaves were lower than those of WT leaves. These analyses also revealed that the content of plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP)-pathway derived cytokinins (CKs) in pgi1 leaves were exceedingly lower than in WT leaves. Noteworthy, exogenous application of CKs largely reverted the low starch content phenotype of pgi1 leaves. The overall data show that pPGI is an important determinant of photosynthesis, energy status, growth and starch accumulation in mesophyll cells likely as a consequence of its involvement in the production of OPPP/glycolysis intermediates necessary for the synthesis of plastidic MEP-pathway derived hormones such as CKs.en
dc.description.sponsorshipThis work was partially supported by the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) [grant numbers BIO2010-18239 and BIO2013-C2-1-P] and by the Government of Navarra [grant number IIM010491.RI1], the Ministry of Education, Youth and Sports of the Czech Republich [Grant L01204 from the National Program of Sustainability] and the European Social Fund and the state budget of the Czech Republic [project POST-UP, reg. No. CZ.1.07/2.3.00/30.0004]. AMS-L acknowledges a predoctoral fellowship from the Spanish Ministry of Science and Innovation. MB acknowledges a post-doctoral fellowship from the Public University of Navarra.en
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dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1371/journal.pone.0119641
dc.identifier.issn1932-6203
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/30544
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPlos One, 10(3): e0119641en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BIO2013-49125-C2-2-P/ES/
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0119641
dc.rights© 2015 Bahaji et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectPlastidic phosphoglucose isomeraseen
dc.subjectStarchen
dc.subjectMesophyll cellsen
dc.subjectPlastidic cytokininsen
dc.subjectArabidopsisen
dc.titlePlastidic phosphoglucose isomerase is an important determinant of starch accumulation in mesophyll cells, growth, photosynthetic capacity, and biosynthesis of plastidic cytokinins in Arabidopsisen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
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