Binding of Bacillus thuringiensis subsp. israelensis Cry4Ba to Cyt1Aa has an important role in synergism

Cantón, Pablo Emiliano; Zanicthe Reyes, Esmeralda; Ruiz de Escudero Fuentemilla, Íñigo; Bravo, Alejandra; Soberón, Mario

Publication:
Binding of Bacillus thuringiensis subsp. israelensis Cry4Ba to Cyt1Aa has an important role in synergism

dc.contributor.author	Cantón, Pablo Emiliano
dc.contributor.author	Zanicthe Reyes, Esmeralda
dc.contributor.author	Ruiz de Escudero Fuentemilla, Íñigo
dc.contributor.author	Bravo, Alejandra
dc.contributor.author	Soberón, Mario
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.contributor.funder	Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa	es
dc.date.accessioned	2019-04-03T07:22:27Z
dc.date.available	2019-04-03T07:22:27Z
dc.date.issued	2011
dc.description.abstract	Bacillus thuringiensis subsp. israelensis (Bti) produces at least four different crystal proteins that are specifically toxic to different mosquito species and that belong to two non-related family of toxins, Cry and Cyt named Cry4Aa, Cry4Ba, Cry11Aa and Cyt1Aa. Cyt1Aa enhances the activity of Cry4Aa, Cry4Ba or Cry11Aa and overcomes resistance of Culex quinquefasciatus populations resistant to Cry11Aa, Cry4Aa or Cry4Ba. Cyt1Aa synergized Cry11Aa by their specific interaction since single point mutants on both Cyt1Aa and Cry11Aa that affected their binding interaction affected their synergistic insecticidal activity. In this work we show that Cyt1Aa loop 6–E K198A, E204A and 7 K225A mutants affected binding and synergism with Cry4Ba. In addition, site directed mutagenesis showed that Cry4Ba domain II loop -8 is involved in binding and in synergism with Cyt1Aa since Cry4Ba SI303-304AA double mutant showed decreased binding and synergism with Cyt1Aa. These data suggest that similarly to the synergism between Cry11Aa and Cyt1Aa toxins, the Cyt1Aa also functions as a receptor for Cry4Ba explaining the mechanism of synergism between these two Bti toxins.	en
dc.description.sponsorship	Research was funded in part through grants from the National Institutes of Health, 1R01 AI066014, DGAPA/UNAM IN218608 and IN210208-N, CONACyT U48631-Q. IRdE received a José Castillejo postdoctoral grant (Spanish Ministry of Education and Science), and a grant for mobility for Teaching and Research Staff of Public University of Navarre, Spain (UPNA).	en
dc.format.extent	14 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1016/j.peptides.2010.06.005
dc.identifier.issn	0196-9781 (Print)
dc.identifier.issn	1873-5169 (Electronic)
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/32801
dc.language.iso	eng	en
dc.publisher	Elsevier	en
dc.relation.ispartof	Peptides 32 (2011) 595–600	en
dc.relation.publisherversion	https://doi.org/10.1016/j.peptides.2010.06.005
dc.rights	© 2010 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess	en
dc.rights.accessRights	Acceso abierto / Sarbide irekia	es
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject	Bacillus thuringiensis	en
dc.subject	Cry toxins	en
dc.subject	Cyt toxins	en
dc.subject	Synergism	en
dc.title	Binding of Bacillus thuringiensis subsp. israelensis Cry4Ba to Cyt1Aa has an important role in synergism	en
dc.type	info:eu-repo/semantics/article	en
dc.type	Artículo / Artikulua	es
dc.type.version	info:eu-repo/semantics/acceptedVersion	en
dc.type.version	Versión aceptada / Onetsi den bertsioa	es
dspace.entity.type	Publication
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relation.isAuthorOfPublication.latestForDiscovery	4d769119-c590-42cc-9cfb-b6aaf0d7e170