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Characterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesis

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dc.contributor.authorBahaji, Abdellatif
dc.contributor.authorBaroja Fernández, Edurne
dc.contributor.authorRicarte Bermejo, Adriana
dc.contributor.authorSánchez López, Ángela Maríaes_ES
dc.contributor.authorMuñoz Pérez, Francisco José
dc.contributor.authorBaslam, Marouane
dc.contributor.authorAlmagro Zabalza, Goizeder
dc.contributor.authorSesma Pascual, María Teresa
dc.contributor.authorPozueta Romero, Javier
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2020-09-16T11:21:47Z
dc.date.available2020-09-16T11:21:47Z
dc.date.issued2015
dc.description.abstractWe characterized multiple knock-out mutants of the four Arabidopsis sucrose phosphate synthase (SPSA1, SPSA2, SPSB and SPSC) isoforms. Despite their reduced SPS activity, spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc mutants displayed wild type (WT) vegetative and reproductive morphology, and showed WT photosynthetic capacity and respiration. In contrast, growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants was reduced compared with WT plants. Furthermore, these plants displayed a high dark respiration phenotype. spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinated and produced aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulated WT levels of nonstructural carbohydrates. spsa1/spsc leaves possessed high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulated high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions. Results presented in this work show that SPS is essential for plant viability, reveal redundant functions of the four SPS isoforms in processes that are important for plant growth and nonstructural carbohydrate metabolism, and strongly indicate that accelerated starch turnover and enhanced respiration can alleviate the blockage of sucrose biosynthesis in spsa1/spsc leaves.en
dc.description.sponsorshipThis work was partially supported by the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) [grant numbers BIO2010-18239, BIO2013-49125-C2-1-P, BIO2008-02292 and BIO2011-28847-C02-02]. A.M.S-L. acknowledges a predoctoral fellowship from the Spanish Ministry of Science and Innovation. M.B. acknowledges a post-doctoral fellowship from the Public University of Navarra.en
dc.format.extent52 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1016/j.plantsci.2015.06.009
dc.identifier.issn0168-9452
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/38129
dc.language.isoengen
dc.publisherElsevieren
dc.relation.ispartofPlant Science, 2015, 238, 135-147en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BIO2013-49125-C2-2-P/ES/en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//BIO2011-28847-C02-02/ES/en
dc.relation.publisherversionhttps://doi.org/10.1016/j.plantsci.2015.06.009
dc.rights© 2015 Elsevier Ireland Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCarbohydrate metabolismen
dc.subjectDevelopmenten
dc.subjectFunctional interactionen
dc.subjectGenetic redundancyen
dc.subjectGrowthen
dc.subjectenSucroseen
dc.titleCharacterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesisen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dc.type.versionVersión aceptada / Onetsi den bertsioaes
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