Base pairing interaction between 5′- and 3′-UTRs controls icaR mRNA translation in Staphylococcus aureus

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dc.contributor.authorRuiz de los Mozos Aliaga, Igor
dc.contributor.authorVergara Irigaray, Marta
dc.contributor.authorSegura, Víctor
dc.contributor.authorVillanueva San Martín, Maite
dc.contributor.authorBitarte Manzanal, Nerea
dc.contributor.authorSaramago, Margarida
dc.contributor.authorDomingues, Susana
dc.contributor.authorArraiano, Cecilia M.
dc.contributor.authorFechter, Pierre
dc.contributor.authorRomby, Pascale
dc.contributor.authorValle Turrillas, Jaione
dc.contributor.authorSolano Goñi, Cristina
dc.contributor.authorLasa Uzcudun, Íñigo
dc.contributor.authorToledo Arana, Alejandro
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2015-12-22T08:50:33Z
dc.date.available2015-12-22T08:50:33Z
dc.date.issued2013
dc.descriptionUPNa. Instituto de Agrobiotecnología. Laboratorio de Biofilms Microbianos.es_ES
dc.descriptionIncluye 10 ficheros de datoses_ES
dc.description.abstractThe presence of regulatory sequences in the 39 untranslated region (39-UTR) of eukaryotic mRNAs controlling RNA stability and translation efficiency is widely recognized. In contrast, the relevance of 39-UTRs in bacterial mRNA functionality has been disregarded. Here, we report evidences showing that around one-third of the mapped mRNAs of the major human pathogen Staphylococcus aureus carry 39-UTRs longer than 100-nt and thus, potential regulatory functions. We selected the long 39-UTR of icaR, which codes for the repressor of the main exopolysaccharidic compound of the S. aureus biofilm matrix, to evaluate the role that 39-UTRs may play in controlling mRNA expression. We showed that base pairing between the 39- UTR and the Shine-Dalgarno (SD) region of icaR mRNA interferes with the translation initiation complex and generates a double-stranded substrate for RNase III. Deletion or substitution of the motif (UCCCCUG) within icaR 39-UTR was sufficient to abolish this interaction and resulted in the accumulation of IcaR repressor and inhibition of biofilm development. Our findings provide a singular example of a new potential post-transcriptional regulatory mechanism to modulate bacterial gene expression through the interaction of a 39-UTR with the 59-UTR of the same mRNA.en
dc.description.sponsorshipThis work was supported by grants from Spanish Ministry of Economy and Competitiveness (BFU2011-23222, BIO2008-05284-C02-01 and ERA-NET Pathogenomics PIM2010EPA-00606) and from Fundação para a Ciência e Tecnologia - Portugal (ERA-PTG/0002/2010 and PEst-OE/EQB/LA0004/2011). IRdlM and JV were supported by F.P.I. (BES-2009-017410) and Ramón y Cajal (RYC-2009-03948) contracts, respectively, from the Spanish Ministry of Economy and Competitiveness.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1371/journal.pgen.1004001
dc.identifier.issn1553-7390 (Print)
dc.identifier.issn1553-7404 (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/19768
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPLoS Genetics 9(12): e1004001en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/BFU2011-23222/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//BIO2008-05284-C02-01/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//BIO2008-05284-C02-01/ES/
dc.relation.publisherversionhttps://dx.doi.org/10.1371/journal.pgen.1004001
dc.rights© 2013 Ruiz de los Mozos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectBase pairing interactionen
dc.subjectStaphylococcus aureusen
dc.subjectmRNA expressionen
dc.subject5-UTRen
dc.subject3-UTRen
dc.subjecticaRen
dc.titleBase pairing interaction between 5′- and 3′-UTRs controls icaR mRNA translation in Staphylococcus aureusen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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