Publication: Sensitivity of the ISO 6579:2002/Amd 1:2007 standard method for detection of Salmonella spp. on mesenteric lymph nodes from slaughter pigs
| dc.contributor.author | Mainar Jaime, Raúl Carlos | |
| dc.contributor.author | Andrés, S. | |
| dc.contributor.author | Vico, J. P. | |
| dc.contributor.author | San Román Aberasturi, Beatriz | |
| dc.contributor.author | Garrido González, Victoria | |
| dc.contributor.author | Grilló Dolset, María Jesús | |
| dc.contributor.department | IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua | es_ES |
| dc.contributor.funder | Gobierno de Navarra / Nafarroako Gobernua, IIQ14064.RI1 | es |
| dc.contributor.funder | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |
| dc.date.accessioned | 2019-01-21T13:24:46Z | |
| dc.date.available | 2019-01-21T13:24:46Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | The ISO 6579:2002/Amd 1:2007 (ISO) standard has been the bacteriological standard method used in the European Union for the detection of Salmonella spp. in pig mesenteric lymph nodes (MLN), but there are no published estimates of the diagnostic sensitivity (Se) of the method in this matrix. Here, the Se of the ISO (SeISO) was estimated on 675 samples selected from two populations with different Salmonella prevalences (14 farms with a ≥20% prevalence and 13 farms with a<20% prevalence) and through the use of latent-class models in concert with Bayesian inference, assuming 100% ISO specificity, and an invA-based PCR as the second diagnostic method. The SeISO was estimated to be close to 87%, while the sensitivity of the PCR reached up to 83.6% and its specificity was 97.4%. Interestingly, the bacteriological reanalysis of 33 potential false-negative (PCR-positive) samples allowed isolation of 19 (57.5%) new Salmonella strains, improving the overall diagnostic accuracy of the bacteriology. Considering the usual limitations of bacteriology regarding Se, these results support the adequacy of the ISO for the detection of Salmonella spp. from MLN and also that of the PCR-based method as an alternative or complementary (screening) test for the diagnosis of pig salmonellosis, particularly considering the cost and time benefits of the molecular procedure. | en |
| dc.description.sponsorship | This study was funded by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) of Spain (research grants no. RTA2007-65 and FAU2008-16) and the Gobierno de Navarra (project reference IIQ14064.RI1). A fellowship for V.G. was funded by the Public University of Navarre, and B.S.R. received a contract from CSIC (JAEDoc program). | en |
| dc.format.extent | 6 p. | |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | 10.1128/jcm.02099-12 | |
| dc.identifier.issn | 0095-1137 (Print) | |
| dc.identifier.issn | 1098-660X (Electronic) | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/32032 | |
| dc.language.iso | eng | en |
| dc.publisher | American Society for Microbiology | en |
| dc.relation.ispartof | Journal of Clinical Microbiology, January 2013 Volume 51 Number 1, p. 89–94 | en |
| dc.relation.publisherversion | https://doi.org/10.1128/jcm.02099-12 | |
| dc.rights | © 2013, American Society for Microbiology. All Rights Reserved. | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
| dc.rights.accessRights | Acceso abierto / Sarbide irekia | es |
| dc.subject | ISO 6579:2002/Amd 1:2007 | en |
| dc.subject | Salmonella spp. | en |
| dc.subject | Mesenteric lymph nodes | en |
| dc.subject | Pigs | en |
| dc.subject | Salmonellosis | en |
| dc.title | Sensitivity of the ISO 6579:2002/Amd 1:2007 standard method for detection of Salmonella spp. on mesenteric lymph nodes from slaughter pigs | en |
| dc.type | info:eu-repo/semantics/article | en |
| dc.type | Artículo / Artikulua | es |
| dc.type.version | info:eu-repo/semantics/publishedVersion | en |
| dc.type.version | Versión publicada / Argitaratu den bertsioa | es |
| dspace.entity.type | Publication | |
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