Coordinated cyclic-di-GMP repression of salmonella motility through YcgR and cellulose

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dc.contributor.authorZorraquino Salvo, Violeta
dc.contributor.authorGarcía Martínez, Begoña
dc.contributor.authorLatasa Osta, Cristina
dc.contributor.authorEcheverz Sarasúa, Maite
dc.contributor.authorToledo Arana, Alejandro
dc.contributor.authorValle Turrillas, Jaione
dc.contributor.authorLasa Uzcudun, Íñigo
dc.contributor.authorSolano Goñi, Cristina
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua: 1312/2010es
dc.date.accessioned2016-11-04T15:31:44Z
dc.date.available2016-11-04T15:31:44Z
dc.date.issued2013
dc.descriptionIncluye 1 fichero de datoses_ES
dc.description.abstractCyclic di-GMP (c-di-GMP) is a secondary messenger that controls a variety of cellular processes, including the switch between a biofilm and a planktonic bacterial lifestyle. This nucleotide binds to cellular effectors in order to exert its regulatory functions. In Salmonella, two proteins, BcsA and YcgR, both of them containing a c-di-GMP binding PilZ domain, are the only known c-di-GMP receptors. BcsA, upon c-di-GMP binding, synthesizes cellulose, the main exopolysaccharide of the biofilm matrix. YcgR is dedicated to c-di-GMP-dependent inhibition of motility through its interaction with flagellar motor proteins. However, previous evidences indicate that in the absence of YcgR, there is still an additional element that mediates motility impairment under high c-di-GMP levels. Here we have uncovered that cellulose per se is the factor that further promotes inhibition of bacterial motility once high c-di-GMP contents drive the activation of a sessile lifestyle. Inactivation of different genes of the bcsABZC operon, mutation of the conserved residues in the RxxxR motif of the BcsA PilZ domain, or degradation of the cellulose produced by BcsA rescued the motility defect of ΔycgR strains in which high c-di-GMP levels were reached through the overexpression of diguanylate cyclases. High c-di-GMP levels provoked cellulose accumulation around cells that impeded flagellar rotation, probably by means of steric hindrance, without affecting flagellum gene expression, exportation, or assembly. Our results highlight the relevance of cellulose in Salmonella lifestyle switching as an architectural element that is both essential for biofilm development and required, in collaboration with YcgR, for complete motility inhibition.en
dc.description.sponsorshipJ.V. was supported by a Ramón y Cajal contract from the Ministerio de Economía y Competitividad, Spain. A JAE predoctoral research contract for V.Z. from the Consejo Superior de Investigaciones Científicas (CSIC, Spain) is gratefully acknowledged. This research was supported by a grant from the Departamento de Salud (Resolución 1312/2010), Gobierno de Navarra, Spain.en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1128/JB.01789-12
dc.identifier.issn0021-9193 (Print)
dc.identifier.issn1098-5530 (Electronic)
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/22577
dc.language.isoengen
dc.publisherAmerican Society for Microbiologyen
dc.relation.ispartofJournal of Bacteriology, February 2013, vol. 195, no. 3, 417-428en
dc.relation.publisherversionhttps://dx.doi.org/10.1128/JB.01789-12
dc.rights© 2013, American Society for Microbiology. All Rights Reserved.en
dc.rights© del material complementario: los autoreses_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectCyclic di-GMPen
dc.subjectSalmonellaen
dc.subjectCelluloseen
dc.subjectYcgRen
dc.subjectMotilityen
dc.subjectBiofilmsen
dc.titleCoordinated cyclic-di-GMP repression of salmonella motility through YcgR and celluloseen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery7f89244d-67ab-4c4d-b868-ad7ae9112055

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