Development of an aptamer based luminescent optical fiber sensor for the continuous monitoring of Hg2+ in aqueous media

Acha Morrás, Nerea de; Elosúa Aguado, César; Arregui San Martín, Francisco Javier

Development of an aptamer based luminescent optical fiber sensor for the continuous monitoring of Hg2+ in aqueous media

dc.contributor.author	Acha Morrás, Nerea de
dc.contributor.author	Elosúa Aguado, César
dc.contributor.author	Arregui San Martín, Francisco Javier
dc.contributor.department	Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren	eu
dc.contributor.department	Institute of Smart Cities - ISC	en
dc.contributor.department	Ingeniería Eléctrica, Electrónica y de Comunicación	es_ES
dc.date.accessioned	2020-07-06T08:41:23Z
dc.date.available	2020-07-06T08:41:23Z
dc.date.issued	2020
dc.description.abstract	A fluorescent optical fiber sensor for the detection of mercury (Hg2+) ions in aqueous solutions is presented in this work. The sensor was based on a fluorophore-labeled thymine (T)-rich oligodeoxyribonucleotide (ON) sequence that was directly immobilized onto the tip of a tapered optical fiber. In the presence of mercury ions, the formation of T–Hg2+-T mismatches quenches the fluorescence emission by the labeled fluorophore, which enables the measurement of Hg2+ ions in aqueous solutions. Thus, in contrast to commonly designed sensors, neither a fluorescence quencher nor a complementary ON sequence is required. The sensor presented a response time of 24.8 seconds toward 5 × 10−12 M Hg2+. It also showed both good reversibility (higher than the 95.8%) and selectivity: the I0 /I variation was 10 times higher for Hg2+ ions than for Mn2+ ions. Other contaminants examined (Co2+, Ag+, Cd2+, Ni2+, Ca2+, Pb2+, Mn2+, Zn2+, Fe3+, and Cu2+) presented an even lower interference. The limit of detection of the sensor was 4.73 × 10−13 M Hg2+ in buffer solution and 9.03 × 10−13 M Hg2+ in ultrapure water, and was also able to detect 5 × 10−12 M Hg2+ in tap water.	en
dc.description.sponsorship	This work was supported by the Spanish State Research Agency (AEI) and the Spanish Ministry of Economy and Competitiveness through the TEC2016-79367-C2-2-R project.	en
dc.format.extent	13 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.3390/s20082372
dc.identifier.issn	1424-8220
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/37338
dc.language.iso	eng	en
dc.publisher	MDPI	en
dc.relation.ispartof	Sensors, 2020, 20(8), 2372	en
dc.relation.projectID	info:eu-repo/grantAgreement/ES/1PE/TEC2016-79367/
dc.relation.publisherversion	https://doi.org/10.3390/s20082372
dc.rights	© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Optical fiber biosensor	en
dc.subject	Luminescent biosensor	en
dc.subject	Fluorophore-labelled aptamer	en
dc.subject	Mercury detection	en
dc.title	Development of an aptamer based luminescent optical fiber sensor for the continuous monitoring of Hg2+ in aqueous media	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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