Viral load, tissue distribution and histopathological lesions in goats naturally and experimentally infected with the Small Ruminant Lentivirus Genotype E (subtype E1 Roccaverano strain)

dc.contributor.authorGrego, E.
dc.contributor.authorReina Arias, Ramsés
dc.contributor.authorLanfredini, S.
dc.contributor.authorTursi, M.
dc.contributor.authorFavole, A.
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2020-11-11T10:25:00Z
dc.date.available2020-11-11T10:25:00Z
dc.date.issued2018
dc.description.abstractSmall Ruminant Lentivirus (SRLV) subtype E1, also known as Roccaverano strain, is considered a low pathogenic virus on the basis of natural genetic deletions, in vitro properties and on-farm observations. In order to gain more knowledge on this atypical lentivirus we investigated the in vivo tropism of Roccaverano strain in both, experimentally and naturally infected goats. Antibody responses were monitored as well as tissue distribution and viral load, evaluated by real time PCR on single spliced (gag/env) and multiple spliced (rev) RNA targets respectively, that were compared to histopathological lesions. Lymph nodes, spleen, alveolar macrophages and mammary gland turned out to be the main tissue reservoirs of genotype E1-provirus. Moreover, mammary gland and/or mammary lymph nodes acted as active replication sites in dairy goats, supporting the lactogenic transmission of this virus. Notably, a direct association between viral load and concomitant infection or inflammatory processes was evident within organs such as spleen, lung and testis. Our results validate the low pathogenicity designation of SRLV genotype E1 in vivo, and confirm the monocyte-macrophage cell lineage as the main virus reservoir of this genotype. Accordingly, SRLV genotype E displays a tropism towards all tissues characterized by an abundant presence of these cells, either for their own anatomical structure or for an occasional infectious/inflammatory status.en
dc.description.sponsorshipThis work was co-funded by the Italian Ministry of Instruction, University and Research PRIN 2008 (no. 20084CSFLT), by Piedmont Region, 'Ricerca Sanitaria Finalizzata' 2008 and 2009, and by University of Turin, 'Fondi ricerca locale (ex-60%)' 2009. R. Reina was supported by Spanish Ministry of Science and Innovation 'Ramón y Cajal' contract (AGL2013-49137-C3-1R).en
dc.format.extent22 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1016/j.rvsc.2018.01.008
dc.identifier.issn0034-5288
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/38640
dc.language.isoengen
dc.publisherElsevieren
dc.relation.ispartofResearch in Veterinary Science, 2018, 118, 107-114en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2013-49137-C3-1-R/ES/
dc.relation.publisherversionhttps://doi.org/10.1016/j.rvsc.2018.01.008
dc.rights© 2018 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectVirusesen
dc.subjectSmall Ruminant Lentivirus Genotype Een
dc.subjectGoatsen
dc.titleViral load, tissue distribution and histopathological lesions in goats naturally and experimentally infected with the Small Ruminant Lentivirus Genotype E (subtype E1 Roccaverano strain)en
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublication
relation.isAuthorOfPublication5eb332ef-80cd-4c2b-942f-e48c9d3725f2
relation.isAuthorOfPublication.latestForDiscovery5eb332ef-80cd-4c2b-942f-e48c9d3725f2

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