Lossy mode resonance-based optical immunosensor towards detecting gliadin in aqueous solutions
| dc.contributor.author | Benítez Pérez, Melanys | |
| dc.contributor.author | Zubiate Orzanco, Pablo | |
| dc.contributor.author | Socorro Leránoz, Abián Bentor | |
| dc.contributor.author | Matías Maestro, Ignacio | |
| dc.contributor.department | Ingeniería Eléctrica, Electrónica y de Comunicación | es_ES |
| dc.contributor.department | Institute of Smart Cities - ISC | en |
| dc.contributor.department | Ingeniaritza Elektrikoa, Elektronikoaren eta Telekomunikazio Ingeniaritzaren | eu |
| dc.contributor.funder | Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa | es |
| dc.date.accessioned | 2023-02-27T12:24:20Z | |
| dc.date.available | 2023-02-27T12:24:20Z | |
| dc.date.issued | 2023 | |
| dc.date.updated | 2023-02-27T12:13:47Z | |
| dc.description.abstract | The development of accurate, intuitive, and easy-to-handle devices to detect different types of allergens is on the rise, as these are useful tools to guarantee consumer safety, which should be a priority for any food industry. Gliadin, one of the main proteins present in gluten, is the one responsible for triggering the immune system to produce autoantibodies in celiac disease, the most dangerous pathology related to gluten. Lossy Mode Resonance (LMR) based biosensors are lately known as a promising sensing technology and its implementation on planar waveguides has been shown to result in manageable, sustainable and robust structures. In this work, an LMR based microfluidic biosensor for gliadin detection is proposed, by coating a coverslip with Titanium Dioxide (TiO2) by Atomic Layer Deposition (ALD) to generate the resonance phenomena and functionalizing the sensor surface with anti-gliadin antibody (AGA) through covalent bond. The sensor was exposed to different gliadin concentrations in ultrapure water, in the range of 0.1–100 ppm with an accuracy of ±0.14 ppm, for a sensitivity of 1.35 ppm/ml. The calibration curve was obtained from the experimental data corresponding to three repetitions of the assay and a limit of detection (LOD) of 0.05 ppm was achieved. Moreover, the sensor was exposed to commercial flour samples, some of them labeled as gluten free (GF) and the response agreed with the expected results according to product label. Biosensor specificity to gliadin was demonstrated by injecting chicken egg white albumin without obtaining any significant response. | en |
| dc.description.sponsorship | Authors would like to thank the partial funding by the Spanish Agencia Estatal de Investigacion (AEI) with references PID2019-106070RB-I00 and also by the Public University of Navarra, with the project BIOFOCEL (ref. PJUPNA2033) and a pre-doctoral research grant. | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Benítez, M., Zubiate, P., Socorro-Leránoz, A. B., & Matias, I. R. (2023). Lossy mode resonance-based optical immunosensor towards detecting gliadin in aqueous solutions. Food Control, 147, 109624. https://doi.org/10.1016/j.foodcont.2023.109624 | en |
| dc.identifier.doi | 10.1016/j.foodcont.2023.109624 | |
| dc.identifier.issn | 0956-7135 | |
| dc.identifier.uri | https://academica-e.unavarra.es/handle/2454/44827 | |
| dc.language.iso | eng | en |
| dc.publisher | Elsevier | en |
| dc.relation.ispartof | Food control 147 (2023) 109624 | en |
| dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106070RB-I00/ES/ | |
| dc.relation.publisherversion | https://doi.org/10.1016/j.foodcont.2023.109624 | |
| dc.rights | © 2023 The Authors. This is an open access article under the CC BY-NC-ND license | en |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Gluten | en |
| dc.subject | Gliadin | en |
| dc.subject | Food safety | en |
| dc.subject | Lossy mode resonance | en |
| dc.subject | Biosensor | en |
| dc.subject | Planar waveguide | en |
| dc.title | Lossy mode resonance-based optical immunosensor towards detecting gliadin in aqueous solutions | en |
| dc.type | info:eu-repo/semantics/article | |
| dc.type.version | info:eu-repo/semantics/publishedVersion | |
| dspace.entity.type | Publication | |
| relation.isAuthorOfPublication | 5362455c-45c7-4da4-9627-218e3bc1b1f1 | |
| relation.isAuthorOfPublication | 88f69525-b7db-404e-97ad-b18e8cf0afc9 | |
| relation.isAuthorOfPublication | bbb769e0-e56c-4b53-8e0b-cf33da20a35d | |
| relation.isAuthorOfPublication.latestForDiscovery | 5362455c-45c7-4da4-9627-218e3bc1b1f1 |