Soybean inoculated with one bradyrhizobium strain isolated at elevated [CO2] show an impaired C and N metabolism when grown at ambient [CO2]

Soba Hidalgo, David; Aranjuelo Michelena, Iker; Gakière, Bertrand; Gilard, Françoise; Pérez López, Usue; Mena Petite, Amaia; Muñoz Rueda, Alberto; Lacuesta, Maite; Sanz Sáez, Álvaro

Soybean inoculated with one bradyrhizobium strain isolated at elevated [CO2] show an impaired C and N metabolism when grown at ambient [CO2]

dc.contributor.author	Soba Hidalgo, David
dc.contributor.author	Aranjuelo Michelena, Iker
dc.contributor.author	Gakière, Bertrand
dc.contributor.author	Gilard, Françoise
dc.contributor.author	Pérez López, Usue
dc.contributor.author	Mena Petite, Amaia
dc.contributor.author	Muñoz Rueda, Alberto
dc.contributor.author	Lacuesta, Maite
dc.contributor.author	Sanz Sáez, Álvaro
dc.contributor.department	Ciencias	es_ES
dc.contributor.department	Zientziak	eu
dc.date.accessioned	2022-01-18T09:18:38Z
dc.date.available	2022-01-18T09:18:38Z
dc.date.issued	2021
dc.description.abstract	Soybean (Glycine max L.) future response to elevated [CO2] has been shown to differ when inoculated with B. japonicum strains isolated at ambient or elevated [CO2]. Plants, inoculated with three Bradyrhizobium strains isolated at different [CO2], were grown in chambers at current and elevated [CO2] (400 vs. 700 ppm). Together with nodule and leaf metabolomic profile, characterization of nodule N-fixation and exchange between organs were tested through 15N2-labeling analysis. Soybeans inoculated with SFJ14-36 strain (isolated at elevated [CO2]) showed a strong metabolic imbalance, at nodule and leaf levels when grown at ambient [CO2], probably due to an insufficient supply of N by nodules, as shown by 15N2-labeling. In nodules, due to shortage of photoassimilate, C may be diverted to aspartic acid instead of malate in order to improve the efficiency of the C source sustaining N2-fixation. In leaves, photorespiration and respiration were boosted at ambient [CO2] in plants inoculated with this strain. Additionally, free phytol, antioxidants, and fatty acid content could be indicate induced senescence due to oxidative stress and lack of nitrogen. Therefore, plants inoculated with Bradyrhizobium strain isolated at elevated [CO2] may have lost their capacity to form effective symbiosis at ambient [CO2] and that was translated at whole plant level through metabolic impairment.	en
dc.description.sponsorship	This work was financially supported by the following grants: GRUPO Gobierno Vasco IT1022-16 and projects 32-2016-00043, 37-2017-00047, and 000049-IDA2019-38 from the Economic Development and Infrastructures Department of the Basque Country, Spain.	en
dc.format.extent	16 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.3389/fpls.2021.656961
dc.identifier.issn	1664-462X
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/41835
dc.language.iso	eng	en
dc.publisher	Frontiers Media	en
dc.relation.ispartof	Frontiers in Plant Science 12:656961	en
dc.relation.publisherversion	http://doi.org/10.3389/fpls.2021.656961
dc.rights	© 2021 Soba, Aranjuelo, Gakière, Gilard, Pérez-López, Mena-Petite, Muñoz-Rueda, Lacuesta and Sanz-Saez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Bradyrhizobium strains	en
dc.subject	C and N metabolism	en
dc.subject	Elevated [CO2]	en
dc.subject	Metabolomics	en
dc.subject	N-fixation	en
dc.subject	Nodule	en
dc.subject	Soybean	en
dc.title	Soybean inoculated with one bradyrhizobium strain isolated at elevated [CO2] show an impaired C and N metabolism when grown at ambient [CO2]	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
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