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Genetic and isotope ratio mass spectrometric evidence for the occurrence of starch degradation and cycling in illuminated Arabidopsis leaves

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dc.contributor.authorBaslam, Marouane
dc.contributor.authorBaroja Fernández, Edurne
dc.contributor.authorRicarte Bermejo, Adriana
dc.contributor.authorSánchez López, Ángela María
dc.contributor.authorAranjuelo Michelena, Iker
dc.contributor.authorBahaji, Abdellatif
dc.contributor.authorMuñoz Pérez, Francisco José
dc.contributor.authorAlmagro Zabalza, Goizeder
dc.contributor.authorPujol, Pablo
dc.contributor.authorGalarza, Regina
dc.contributor.authorTeixidor, Pilar
dc.contributor.authorPozueta Romero, Javier
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2018-09-06T12:20:19Z
dc.date.available2018-09-06T12:20:19Z
dc.date.issued2017
dc.description.abstractAlthough there is a great wealth of data supporting the occurrence of simultaneous synthesis and breakdown of storage carbohydrate in many organisms, previous 13CO2 pulse-chase based studies indicated that starch degradation does not operate in illuminated Arabidopsis leaves. Here we show that leaves of gwd, sex4, bam4, bam1/bam3 and amy3/isa3/lda starch breakdown mutants accumulate higher levels of starch than wild type (WT) leaves when cultured under continuous light (CL) conditions. We also show that leaves of CL grown dpe1 plants impaired in the plastidic disproportionating enzyme accumulate higher levels of maltotriose than WT leaves, the overall data providing evidence for the occurrence of extensive starch degradation in illuminated leaves. Moreover, we show that leaves of CL grown mex1/ pglct plants impaired in the chloroplastic maltose and glucose transporters display a severe dwarf phenotype and accumulate high levels of maltose, strongly indicating that the MEX1 and pGlcT transporters are involved in the export of starch breakdown products to the cytosol to support growth during illumination. To investigate whether starch breakdown products can be recycled back to starch during illumination through a mechanism involving ADP-glucose pyrophosphorylase (AGP) we conducted kinetic analyses of the stable isotope carbon composition (δ13C) in starch of leaves of 13CO2 pulsed-chased WT and AGP lacking aps1 plants. Notably, the rate of increase of δ13C in starch of aps1 leaves during the pulse was exceedingly higher than that of WT leaves. Furthermore, δ13C decline in starch of aps1 leaves during the chase was much faster than that of WT leaves, which provides strong evidence for the occurrence of AGP-mediated cycling of starch breakdown products in illuminated Arabidopsis leaves.en
dc.description.sponsorshipComisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) Grant numbers: BIO2010-18239 and BIO2013-49125-C2-1-P.en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.identifier.doi10.1371/journal.pone.0171245
dc.identifier.issn1932-6203
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/30546
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPlos One, 12(2): e0171245.en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BIO2013-49125-C2-2-P/ES/en
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0171245
dc.rights© 2017 Baslam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectArabidopsis leavesen
dc.subjectStarchen
dc.titleGenetic and isotope ratio mass spectrometric evidence for the occurrence of starch degradation and cycling in illuminated Arabidopsis leavesen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dspace.entity.typePublication
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