Expression analysis of lung miRNAs responding to ovine VM virus infection by RNA-seq

Bilbao Arribas, Martin; Abendaño, Naiara; Varela Martínez, Endika; Reina Arias, Ramsés; Andrés Cara, Damián de; Jugo, Begoña M.

Expression analysis of lung miRNAs responding to ovine VM virus infection by RNA-seq

dc.contributor.author	Bilbao Arribas, Martin
dc.contributor.author	Abendaño, Naiara
dc.contributor.author	Varela Martínez, Endika
dc.contributor.author	Reina Arias, Ramsés
dc.contributor.author	Andrés Cara, Damián de
dc.contributor.author	Jugo, Begoña M.
dc.contributor.department	IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua	es_ES
dc.date.accessioned	2019-07-17T12:13:13Z
dc.date.available	2019-07-17T12:13:13Z
dc.date.issued	2019
dc.description.abstract	Background: MicroRNAs (miRNAs) are short endogenous, single-stranded, noncoding small RNA molecules of approximately 22 nucleotides in length. They regulate gene expression posttranscriptionally by silencing mRNA expression, thus orchestrating many physiological processes. The Small Ruminant Lentiviruses (SRLV) group includes the Visna Maedi Virus (VMV) and Caprine Arthritis Encephalitis (CAEV) viruses, which cause a disease in sheep and goats characterized by pneumonia, mastitis, arthritis and encephalitis. Their main target cells are from the monocyte/macrophage lineage. To date, there are no studies on the role of miRNAs in this viral disease. Results: Using RNA-seq technology and bioinformatics analysis, the expression levels of miRNAs during different clinical stages of infection were studied. A total of 212 miRNAs were identified, of which 46 were conserved sequences in other species but found for the first time in sheep, and 12 were completely novel. Differential expression analysis comparing the uninfected and seropositive groups showed changes in several miRNAs; however, no significant differences were detected between seropositive asymptomatic and diseased sheep. The robust increase in the expression level of oar-miR-21 is consistent with its increased expression in other viral diseases. Furthermore, the target prediction of the dysregulated miRNAs revealed that they control genes involved in proliferation-related signalling pathways, such as the PI3K-Akt, AMPK and ErbB pathways. Conclusions: To the best of our knowledge, this is the first study reporting miRNA profiling in sheep in response to SRLV infection. The known functions of oar-miR-21 as a regulator of inflammation and proliferation appear to be a possible cause of the lesions caused in the sheep's lungs. This miRNA could be an indicator for the severity of the lung lesions, or a putative target for therapeutic intervention.	en
dc.description.sponsorship	This work was supported by a UPV/EHU grant (GIU14/23) provided to B.M. Jugo, two predoctoral fellowships from the UPV/EHU to M. Bilbao-Arribas (PIF17/306) and E. Varela-Martínez (PIF15/361) and a postdoctoral fellowship from the UPV/EHU to Dr. N. Abendaño (ESPDOC16/43).	en
dc.format.extent	13 p.
dc.format.mimetype	application/pdf	en
dc.identifier.doi	10.1186/s12864-018-5416-0
dc.identifier.issn	1471-2164
dc.identifier.uri	https://academica-e.unavarra.es/handle/2454/33616
dc.language.iso	eng	en
dc.publisher	BioMed Central	en
dc.relation.ispartof	BMC Genomics, (2019) 20:62	en
dc.relation.publisherversion	https://doi.org/10.1186/s12864-018-5416-0
dc.rights	© The Author(s). 2019 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.	en
dc.rights.accessRights	info:eu-repo/semantics/openAccess
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	Differential expression	en
dc.subject	Host-virus interaction	en
dc.subject	miRNAs	en
dc.subject	RNA-seq	en
dc.subject	Visna-Maedi	en
dc.title	Expression analysis of lung miRNAs responding to ovine VM virus infection by RNA-seq	en
dc.type	info:eu-repo/semantics/article
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication
relation.isAuthorOfPublication	5eb332ef-80cd-4c2b-942f-e48c9d3725f2
relation.isAuthorOfPublication	50c83dcd-b739-4348-b0f0-3a230e16c398
relation.isAuthorOfPublication.latestForDiscovery	5eb332ef-80cd-4c2b-942f-e48c9d3725f2