Publication:
Proteomics analysis reveals non-controlled activation of photosynthesis and protein synthesis in a rice npp1 mutant under high temperature and elevated CO2 conditions

Simple item page
dc.contributor.authorInomata, Takuya
dc.contributor.authorBaslam, Marouane
dc.contributor.authorMasui, Takahiro
dc.contributor.authorKoshu, Tsutomu
dc.contributor.authorTakamatsu, Takeshi
dc.contributor.authorKaneko, Kentaro
dc.contributor.authorPozueta Romero, Javier
dc.contributor.authorMitsui, Toshiaki
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.date.accessioned2019-07-17T12:13:12Z
dc.date.available2019-07-17T12:13:12Z
dc.date.issued2018
dc.description.abstractRice nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) catalyzes the hydrolytic breakdown of the pyrophosphate and phosphodiester bonds of a number of nucleotides including ADP-glucose and ATP. Under high temperature and elevated CO2 conditions (HT + ECO2), the npp1 knockout rice mutant displayed rapid growth and high starch content phenotypes, indicating that NPP1 exerts a negative effect on starch accumulation and growth. To gain further insight into the mechanisms involved in the NPP1 downregulation induced starch overaccumulation, in this study we conducted photosynthesis, leaf proteomic, and chloroplast phosphoproteomic analyses of wild-type (WT) and npp1 plants cultured under HT + ECO2. Photosynthesis in npp1 leaves was significantly higher than in WT. Additionally, npp1 leaves accumulated higher levels of sucrose than WT. The proteomic analyses revealed upregulation of proteins related to carbohydrate metabolism and the protein synthesis system in npp1 plants. Further, our data indicate the induction of 14-3-3 proteins in npp1 plants. Our finding demonstrates a higher level of protein phosphorylation in npp1 chloroplasts, which may play an important role in carbohydrate accumulation. Together, these results offer novel targets and provide additional insights into carbohydrate metabolism regulation under ambient and adverse conditions.en
dc.description.sponsorshipThis research was supported by Grants-in-Aid for Scientific Research (A) (15H02486) and Strategic International Collaborative Research Program by the Japan Science and Technology Agency (JST SICORP).en
dc.format.extent18 p.
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.3390/ijms19092655
dc.identifier.issn1422-0067
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/33613
dc.language.isoengen
dc.publisherMDPIen
dc.relation.ispartofInternational Journal of Molecular Sciences, 2018, 19, 2655en
dc.relation.publisherversionhttps://doi.org/10.3390/ijms19092655
dc.rights© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectChloroplasten
dc.subjectElevated CO2en
dc.subjectHeat stressen
dc.subjectNucleotide pyrophosphatase/phosphodiesteraseen
dc.subject(Phospho)Proteomicsen
dc.subjectPhotosynthesisen
dc.subjectProtein phosphorylationen
dc.subject14-3-3 proteinsen
dc.subjectOryza sativa L.en
dc.subjectStarchen
dc.subjectSucroseen
dc.titleProteomics analysis reveals non-controlled activation of photosynthesis and protein synthesis in a rice npp1 mutant under high temperature and elevated CO2 conditionsen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dspace.entity.typePublication
relation.isAuthorOfPublication44f8348d-8a3c-4f89-bbaa-8303920682c3
relation.isAuthorOfPublication80c8bd69-4127-4474-a679-3b10747cae42
relation.isAuthorOfPublication.latestForDiscovery44f8348d-8a3c-4f89-bbaa-8303920682c3

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
125_Inomata_ProteomicsAnalysis.pdf
Size:
3.01 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download

Collections

Artículos de revista IdAB - IdAB Aldizkari artikuluak
Artículos de revista - Aldizkari artikuluak