Study of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheep

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dc.contributor.authorRamírez Álvarez, Hugo
dc.contributor.authorReina Arias, Ramsés
dc.contributor.authorBertolotti, Luigi
dc.contributor.authorCenoz García, Amaia
dc.contributor.authorHernández, Mirna Margarita
dc.contributor.authorSan Román Aberasturi, Beatriz
dc.contributor.authorGlaría Ezquer, Idoia
dc.contributor.authorAndrés, Ximena de
dc.contributor.authorCrespo Otano, Helena
dc.contributor.authorJauregui, Paula
dc.contributor.authorBenavides, Julio
dc.contributor.authorPolledo, Laura
dc.contributor.authorPérez, Valentín
dc.contributor.authorGarcía Marín, Juan F.
dc.contributor.authorRosati, Sergio
dc.contributor.authorAmorena Zabalza, Beatriz
dc.contributor.authorAndrés Cara, Damián de
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes_ES
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua: IIQ010449.RI1es
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua: IIQ14064.RI1es
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoaes
dc.date.accessioned2014-06-13T11:37:38Z
dc.date.available2014-06-13T11:37:38Z
dc.date.issued2012
dc.description.abstractBackground: A central nervous system (CNS) disease outbreak caused by small ruminant lentiviruses (SRLV) has triggered interest in Spain due to the rapid onset of clinical signs and relevant production losses. In a previous study on this outbreak, the role of LTR in tropism was unclear and env encoded sequences, likely involved in tropism, were not investigated. This study aimed to analyze heterogeneity of SRLV Env regions - TM amino terminal and SU V4, C4 and V5 segments - in order to assess virus compartmentalization in CNS. Results: Eight Visna (neurologically) affected sheep of the outbreak were used. Of the 350 clones obtained after PCR amplification, 142 corresponded to CNS samples (spinal cord and choroid plexus) and the remaining to mammary gland, blood cells, bronchoalveolar lavage cells and/or lung. The diversity of the env sequences from CNS was 11.1-16.1% between animals and 0.35-11.6% within each animal, except in one animal presenting two sequence types (30% diversity) in the CNS (one grouping with those of the outbreak), indicative of CNS virus sequence heterogeneity. Outbreak sequences were of genotype A, clustering per animal and compartmentalizing in the animal tissues. No CNS specific signature patterns were found. Conclusions: Bayesian approach inferences suggested that proviruses from broncoalveolar lavage cells and peripheral blood mononuclear cells represented the common ancestors (infecting viruses) in the animal and that neuroinvasion in the outbreak involved microevolution after initial infection with an A-type strain. This study demonstrates virus compartmentalization in the CNS and other body tissues in sheep presenting the neurological form of SRLV infection.en
dc.description.sponsorshipThis study was supported by grants from Spanish CICYT (AGL2007-66874-C04, AGL2010-22341-C04) and Government of Navarra Department of Agriculture (IIQ010449.RI1 and IIQ14064.RI1). H. Ramirez was supported by PASPA program, UNAM and Public University of Navarra.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doi10.1186/1746-6148-8-8
dc.identifier.issn1746-6148
dc.identifier.other537
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/10864
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.ispartofBCM Veterinary Research, 2012, 8: 8en
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC//AGL2007-66874-C04-01/ES/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//AGL2010-22341-C04-01/ES/
dc.relation.publisherversionhttps://doi.org/10.1186/1746-6148-8-8
dc.rights© 2012 Ramirez et al; BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/
dc.subjectCompartmentalizationen
dc.subjectVisnaen
dc.subjectSmall ruminant lentivirusen
dc.subjectSpinal corden
dc.subjectChoroid plexusen
dc.subjectSheepen
dc.subjectImmunodeficiency virus type 1en
dc.subjectCentral nervous systemen
dc.subjectFemaleen
dc.subjectGenital tracten
dc.subjectMaedi Visnaen
dc.subjectCerebroespinal fluiden
dc.subjectAntiretroviral therapyen
dc.subjectEnvelope glycoproteinen
dc.subjectResistance mutationsen
dc.subjectViral sequencesen
dc.subjectBlooden
dc.titleStudy of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheepen
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dspace.entity.typePublication
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