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Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories

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dc.contributor.authorDučić, Tanjaes_ES
dc.contributor.authorSánchez-Mata, Aliciaes_ES
dc.contributor.authorCastillo-Sánchez, Jesúses_ES
dc.contributor.authorAlgarra González, Manueles_ES
dc.contributor.authorGonzález-Muñoz, Elenaes_ES
dc.contributor.departmentInstitute for Advanced Materials and Mathematics - INAMAT2en
dc.date.accessioned2023-05-10T16:02:05Z
dc.date.available2023-05-10T16:02:05Z
dc.date.issued2023
dc.date.updated2023-05-10T15:50:24Z
dc.description.abstractThe reprogramming of human somatic cells to induced pluripotent cells (iPSCs) has become a milestone and a paradigm shift in the field of regenerative medicine and human disease modeling including drug testing and genome editing. However, the molecular processes occurring during reprogramming and affecting the pluripotent state acquired remain largely unknown. Of interest, different pluripotent states have been described depending on the reprogramming factors used and the oocyte has emerged as a valuable source of information for candidate factors. The present study investigates the molecular changes occurring in somatic cells during reprogramming with either canonical (OSK) or oocyte-based (AOX15) combinations using synchrotron-radiation Fourier transform infrared (SR FTIR) spectroscopy. The data acquired by SR FTIR indicates different representation and conformation of biological relevant macromolecules (lipids, nucleic acids, carbohydrates and proteins) depending on the reprogramming combination used and at different stages during the reprogramming process. Association analysis based on cells spectra suggest that pluripotency acquisition trajectories converge at late intermediate stages while they diverge at early stages. Our results suggest that OSK and AOX15 reprogramming operates through differential mechanisms affecting nucleic acids reorganization and day 10 comes out as a candidate hinge point to further study the molecular pathways involved in the reprogramming process. This study indicates that SR FTIR approach contribute unpaired information to distinguish pluripotent states and to decipher pluripotency acquisition roadmaps and landmarks that will enable advanced biomedical applications of iPSCs.en
dc.description.sponsorshipThe authors thank ALBA Synchrotron facility for beamtime allocation and financial support from the Proposal No. 2021085254 and excellent working conditions. E.G-M acknowledge financial support from Ministerio de Ciencia e Innovación del Gobierno de Espana ˜ (grant number PID2021-124033OB-I00) and from Consejería Economía y Conocimiento Junta de Andalucía-FEDER (grant number UMA18-FEDERJA-107). Funding for open access charge was provided by Universidad de Málaga / CBUA.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDučić, T., Sanchez-Mata, A., Castillo-Sanchez, J., Algarra, M., & Gonzalez-Munoz, E. (2023). Monitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectories. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 297, 122713. https://doi.org/10.1016/j.saa.2023.122713en
dc.identifier.doi10.1016/j.saa.2023.122713
dc.identifier.issn1386-1425
dc.identifier.urihttps://academica-e.unavarra.es/handle/2454/45260
dc.language.isoengen
dc.publisherElsevieren
dc.relation.ispartofSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 297 (2023) 122713en
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI//PID2021-124033OB-I00en
dc.relation.publisherversionhttps://doi.org/10.1016/j.saa.2023.122713
dc.rights© 2023 The Author(s). This is an open access article under the CC BY-NC-ND license.en
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectInduced pluripotent stem cells (iPSCs)en
dc.subjectOocyteen
dc.subjectReprogrammingen
dc.subjectFTIRen
dc.subjectSynchrotron spectroscopyen
dc.titleMonitoring oocyte-based human pluripotency acquisition using synchrotron-based FTIR microspectroscopy reveals specific biomolecular trajectoriesen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dspace.entity.typePublication

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